Enhancing cell therapies from the outside in: Cell surface engineering using synthetic nanomaterials

Stephan, Matthias T.; Irvine, Darrell J.

doi:10.1016/j.nantod.2011.04.001

Cited by 226 publications

(206 citation statements)

References 181 publications

(203 reference statements)

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“…In therapy using MSCs, it is sometimes necessary for the cells to adhere to a culture dish for cell processing, such as during proliferation or the induction of differentiation. 7,26) In such cases, there are 2 possible points where cell-surface modification can be applied: when cultured cells are on the dish or when dispersed cells are in the medium after detachment from the dish. Therefore, we compared the modification efficiency between these 2 conditions.…”

Section: Discussionmentioning

confidence: 99%

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Factors Influencing the Surface Modification of Mesenchymal Stem Cells with Fluorescein-Pegylated Lipids

Takafuji

Higuchi

Muro

et al. 2013

Biological & Pharmaceutical Bulletin

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Artificial introduction of functional molecules on the cell surface may be a promising way to improve the therapeutic effects of cell therapy. Pegylated lipids are conventionally used in drug carriers. The lipid part of pegylated lipids noncovalently interacts with the cell surface. However, little information is available regarding conditions for cell-surface modification by using pegylated lipids. In this study, we synthesized fluorescein-labeled pegylated lipids and evaluated the factors that affect modification efficiency by using human mesenchymal stem cells (hMSCs). As the concentration of the pegylated lipid as well as the exposure time increased, the modification efficiency increased. The modification efficiency at 37°C was 20-and 3-fold higher than that at 4°C and 25°C, respectively. In addition, with an increase in the molecular weight of polyethylene glycol (PEG), more pegylated lipids were extracellularly distributed than those intracellularly distributed. At the optimal condition, pegylated lipids were observed mainly on the cell membrane by confocal microscopy. In contrast, the cell condition (adherent or nonadherent) had little or no effect on the cell-surface modification efficiency. The results of this study will be useful for constructing an optimal modification method for introducing functional molecules on the cell surface.

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Section: Discussionmentioning

confidence: 99%

“…The dynamics of transplanted cells, involving adhesion, migration, and cell-cell interactions, are important factors that affect treatment outcomes of cell therapy. 7) Therefore, the artificial introduction of functional molecules on the cell surface to control cell dynamics is a promising way to improve the therapeutic effects of cell therapy.…”

mentioning

confidence: 99%

Factors Influencing the Surface Modification of Mesenchymal Stem Cells with Fluorescein-Pegylated Lipids

Takafuji

Higuchi

Muro

et al. 2013

Biological & Pharmaceutical Bulletin

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“…Previous investigations have modified the cell surface in an effort to control these interactions with biomolecules and to examine their potential in biomedical applications, such as nucleic acid or protein delivery to cells [1,2], cell delivery to a particular organ [3], and tissue engineering to promote the regeneration of tissues [4]. The methods to modify the cell surface can be grouped into three categories: covalent conjugation; electrostatic interaction; and hydrophobic interaction between amphiphilic molecules with the lipid bilayer of the cell membrane [5,6]. Each of these methods has merits and demerits.…”

Section: Introductionmentioning

confidence: 99%

Interaction between cells and poly(ethylene glycol)-lipid conjugates

Itagaki

Arima

Kuwabara

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15 min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1 × 10(-3) to 1 × 10(-2)molecules/nm(2), depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.

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“…From the fundamental standpoint, the newly emerging concept of functionalizing living cells with synthetic elements [49,50] is a promising new direction for polyelectrolyte multilayer research. For example, by combining the native functions of living immune system cells, such as their ability to target disease sites, with ondemand drug-releasing multilayers, it may be possible to enhance significantly the bodys ability to overcome specific diseases.…”

Section: Multilayer Cellular Backpacksmentioning

confidence: 99%