Enhancing Catalytic Activity of Bioanode for Glucose Biofuel Cell by Compressing Enzyme, Mediator and Carbon Support through Centrifugation

Tan, Desmond C. L.; Sato, Hirotaka

doi:10.1002/chem.201702100

Cited by 8 publications

(7 citation statements)

References 25 publications

(22 reference statements)

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“…The centrifugation step concentrates the CoPc-P4VP system near the GP and likely facilitates physisorption to the particles, which in turn promotes efficient charge transport to the CoPc. 33,57,58 When no GP is present in the film (0 mg cm -2 GP loading), the measured CO2RR activity is relatively constant with increasing CoPc loading (Figure 3a) and there is a corresponding decrease in the normalized activity per CoPc with increasing CoPc loading (Figure 3b). We interpret this result to mean that only a small, constant amount of CoPc near the electrode surface is active as the film loading increases due to inefficient charge transfer to CoPc far from the surface in the film.…”

Section: Resultsmentioning

confidence: 99%

Enhancing Electrochemical Carbon Dioxide Reduction by Polymer-Encapsulated Cobalt Phthalocyanine through Incorporation of Graphite Powder

Soucy

Liu

Eisenberg

et al. 2021

Preprint

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Cobalt phthalocyanine (CoPc) has been extensively studied as a catalyst for the electrochemical reduction of CO2 to value-added products. Previous studies have shown that CoPc is a competent and efficient catalyst when immobilized onto carbon-based electrodes using a polymer binder, especially when immobilized with a graphitic carbon powder support to increase charge transport. In this study, we systematically explore the influence of incorporating graphite powder (GP) into a polymer-encapsulated CoPc on the system’s activity for the electrochemical reduction of CO2. We report a protocol for incorporating GP into CoPc/polymer/GP catalyst films that facilitates physisorption of CoPc to GP, leading to increased activity for CO2 reduction. We show that the activity for CO2 reduction increases with GP loading at low GP loadings, but at sufficiently high GP loadings the activity plateaus as charge transfer is sufficiently fast to no longer be rate limiting. We also demonstrate that axial coordination is still important even in the presence of GP, suggesting that CoPc does not fully coordinate to heteroatoms on the GP surface. We develop a set of optimized conditions under which the CoPc/polymer/GP catalyst systems reduce CO2 with higher activity and similar selectivity to previously reported CoPc/polymer films on edge-plane graphite electrodes. The procedures outlined in this study will be used in future studies to optimize catalyst, polymer, and carbon support loadings for other polymer-catalyst composite systems for electrocatalytic transformations.

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Section: Resultsmentioning

confidence: 99%

Enhancing Electrochemical Carbon Dioxide Reduction by Polymer-Encapsulated Cobalt Phthalocyanine through Incorporation of Graphite Powder

Soucy

Liu

Eisenberg

et al. 2021

Preprint

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“…The biofuel cell with the cross-linking method exhibited a maximum power density of 50 μW cm −2 (Figure 4d) and 30 μW cm −2 in the presence of 50 and 5 mM glucose, respectively, which are comparably high compared to the reported value in the literature that also used platinum wire as the cathode. 54,55 The entrapment one showed a lower power output, which was in agreement with the results of polarization curves of the bioelectrodes.…”

Section: Resultsmentioning

confidence: 99%

A Modifiable, Spontaneously Formed Polymer Gel with Zwitterionic and N-Hydroxysuccinimide Moieties for an Enzymatic Biofuel Cell

Huang

Masuda

Takai

2020

ACS Appl. Polym. Mater.

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A modifiable, spontaneously formed and biocompatible polymer gel, composed of phosphorylcholine and N-hydroxysuccinimide ester, is reported, which can be subjected to postmodification by amine-containing mediators to obtain a redox zwitterionic gel. Owing to attractive interactions between the polymer chains, the network structure of the polymer gel is formed spontaneously in ethanol or water. In addition, the designed hydrogel can serve as an enzyme-immobilizing matrix for enzymatic bioelectrodes. We illustrate the potential applications of the hydrogel with a biofuel cell that demonstrated superior operational stability over 10 days and the unaffected current of the bioelectrode in concentrated protein solution (45 mg mL–1). Compared with a typical redox hydrogel, the zwitterionic moieties with high hydration endow the redox zwitterionic hydrogel with (i) better environment for preserving enzyme’s activity and (ii) resistance to biofouling caused by protein adsorption. These properties contribute to overcoming the most challenging issues in the field of implantable bioelectronic applications, as insufficient operational stability and lack of biocompatibility severely limit their feasibility for practical applications.

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“…Enzymes, as biological macromolecules, are mainly composed of proteins, which can efficiently and selectively catalyze a diverse biochemical reactions [1,2]. They play a notable function in various fields, such as energy production processes, biosensing, the food industry, and biofuels [3][4][5][6]. However, they have some drawbacks, such as product complexity, harsh catalytic conditions and low operational stability because of digestion and denaturation.…”

Section: Introductionmentioning

confidence: 99%

Multienzymes activity of metals and metal oxide nanomaterials: applications from biotechnology to medicine and environmental engineering

Alizadeh

Salimi

2021

J Nanobiotechnol

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With the rapid advancement and progress of nanotechnology, nanomaterials with enzyme-like catalytic activity have fascinated the remarkable attention of researchers, due to their low cost, high operational stability, adjustable catalytic activity, and ease of recycling and reuse. Nanozymes can catalyze the same reactions as performed by enzymes in nature. In contrast the intrinsic shortcomings of natural enzymes such as high manufacturing cost, low operational stability, production complexity, harsh catalytic conditions and difficulties of recycling, did not limit their wide applications. The broad interest in enzymatic nanomaterial relies on their outstanding properties such as stability, high activity, and rigidity to harsh environments, long-term storage and easy preparation, which make them a convenient substitute instead of the native enzyme. These abilities make the nanozymes suitable for multiple applications in sensing and imaging, tissue engineering, environmental protection, satisfactory tumor diagnostic and therapeutic, because of distinguished properties compared with other artificial enzymes such as high biocompatibility, low toxicity, size dependent catalytic activities, large surface area for further bioconjugation or modification and also smart response to external stimuli. This review summarizes and highlights latest progress in applications of metal and metal oxide nanomaterials with enzyme/multienzyme mimicking activities. We cover the applications of sensing, cancer therapy, water treatment and anti-bacterial efficacy. We also put forward the current challenges and prospects in this research area, hoping to extension of this emerging field. In addition to therapeutic potential of nanozymes for disease prevention, their practical effects in diagnostics, to monitor the presence of SARS-CoV-2 and related biomarkers for future pandemics will be predicted.

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Enhancing Catalytic Activity of Bioanode for Glucose Biofuel Cell by Compressing Enzyme, Mediator and Carbon Support through Centrifugation

Cited by 8 publications

References 25 publications

Enhancing Electrochemical Carbon Dioxide Reduction by Polymer-Encapsulated Cobalt Phthalocyanine through Incorporation of Graphite Powder

Enhancing Electrochemical Carbon Dioxide Reduction by Polymer-Encapsulated Cobalt Phthalocyanine through Incorporation of Graphite Powder

A Modifiable, Spontaneously Formed Polymer Gel with Zwitterionic and N-Hydroxysuccinimide Moieties for an Enzymatic Biofuel Cell

Multienzymes activity of metals and metal oxide nanomaterials: applications from biotechnology to medicine and environmental engineering

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