Enhancing cartilage repair with optimized supramolecular hydrogel-based scaffold and pulsed electromagnetic field

Li, Yucong; Li, Linlong; Li, Ye; Feng, Lu; Wang, Bin; Wang, Ming; Wang, Haixing; Zhu, Mingzhao; Yang, Yongkang; Waldorff, Erik I.; Zhang, Nianli; Viohl, Ingmar; Lin, Sien; Bian, Liming; Lee, Wayne; Li, Gang

doi:10.1016/j.bioactmat.2022.10.010

Cited by 18 publications

(15 citation statements)

References 57 publications

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“…In addition, implanted hydrogel in the cartilage defect will swell when it absorbs tissue fluid in the joint cavity, and low‐swelling properties can prevent swelling and displacement of implanted hydrogel during joint motion. [ 34 ] Therefore, low‐swelling hydrogel is critical for PTCD repair to avoid interfering with normal motor function and impairing surrounding tissues and cells. In order to evaluate the swelling properties of the GM/AN 0 , GM/AN 6 , and GM/AN 6 /KGN 50 hydrogels, hydrogel sheets of each group were immersed in phosphate‐buffered saline (PBS) solution.…”

Section: Resultsmentioning

confidence: 99%

Injectable Bioadhesive Photocrosslinkable Hydrogels with Sustained Release of Kartogenin to Promote Chondrogenic Differentiation and Partial‐Thickness Cartilage Defects Repair

Han,

He,

Bai

et al. 2024

Adv Healthcare Materials

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Partial‐thickness cartilage defect (PTCD) is a common and formidable clinical challenge without effective therapeutic approaches. The inherent anti‐adhesive characteristics of the extracellular matrix within cartilage pose a significant impediment to the integration of cells or biomaterials with the native cartilage during cartilage repair. Here, an injectable photocrosslinked bioadhesive hydrogel, consisting of gelatin methacryloy (GM), acryloyl‐6‐aminocaproic acid‐g‐N‐hydroxysuccinimide (AN), and poly (lactic‐co‐glycolic acid) (PLGA) microspheres loaded with kartogenin (KGN) (abbreviated as GM/AN/KGN hydrogel) is designed to enhance interfacial integration and repair of PTCD. After injected in situ at the irregular defect, a stable and robust hydrogel network is rapidly formed by ultraviolet irradiation and it can be quickly and tightly adhered to native cartilage through amide bonds. The hydrogel exhibites good adhesion strength up to 27.25 ± 1.22 kPa by lap shear strength experiments. The GM/AN/KGN hydrogel demonstrates good adhesion, low‐swelling, resistance to fatigue, biocompatibility, and chondrogenesis properties in vitro. A rat model with PTCD exhibits restoration of a smoother surface, stable seamless integration, and abundant aggrecan and type II collagen production. The injectable stable adhesive hydrogel with long‐term chondrogenic differentiation capacity shows great potential to facilitate repair of PTCD.This article is protected by copyright. All rights reserved

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Section: Resultsmentioning

confidence: 99%

Injectable Bioadhesive Photocrosslinkable Hydrogels with Sustained Release of Kartogenin to Promote Chondrogenic Differentiation and Partial‐Thickness Cartilage Defects Repair

Han,

He,

Bai

et al. 2024

Adv Healthcare Materials

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“…PEMF stimulation was applied during the chondrogenic induction of hBMSCs aggregates into micromasses. Our previous study has demonstrated that PEMF stimulation with an FDA approved signal could promote BMSCs chondrogenic differentiation and mitigate cell hypertrophy process [ 27 ]. In this study, we also confirmed that daily PEMF stimulation could effectively promote the chondrogenic differentiation of hBMSCs during micromass culture ( Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…During chondrogenic induction of hBMSCs aggregates, a pulsed electromagnetic field stimulation was utilized to enhance the quality of secreted matrix and secretome by hBMSCs according to our previous publication [ 27 ]. Our previous study has demonstrated that PEMF stimulation with an FDA approved signal (Physio Signal, 10T/s, 15 Hz, Orthofix Medical Inc, USA) could promote BMSCs chondrogenic differentiation and mitigate cell hypertrophy process.…”

Section: Experimental Section/methodsmentioning

confidence: 99%

“…In comparison to biochemical stimuli, biophysical cues such as PEMF could facilitate wider and more systemic effects on stem cells that universally enhance the quality of cells and cell products [ [24] , [25] , [26] ]. In our previous study, we have demonstrated that PEMF could promote the synthesize of chondrogenic ECM and prevent degenerative matrix production [ 27 ]. Therefore, Biophysio-stimulation with PEMF was utilized to enhance the quality and bioactivity of MESS.…”

Section: Introductionmentioning

confidence: 99%

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O-alg-THAM/gel hydrogels functionalized with engineered microspheres based on mesenchymal stem cell secretion recruit endogenous stem cells for cartilage repair

Wang

et al. 2023

Bioactive Materials

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“…According to Li Y et al (51) , PEMF treatment was proven to enhance the quality of engineered chondrogenic constructs in vitro and facilitate chondrogenesis and cartilage repair in vivo. Liu J et al (52) demonstrated that PEMF alleviated the degree of inflammation and degeneration of cartilage in rats with OA, based on the histopathological changes and decline of the expression of IL-1β and MMP-13.…”

Section: Pulsed Electromagnetic Field (Pemf) and Cartilagementioning

confidence: 99%

Applications of Pulsed Electromagnetic Field Therapy in Skeletal-Muscle System: An Integrative Review

et al. 2023

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Background: The Pulsed Electromagnetic Field (PEMF) technology has attracted increasing interest since consistent evidence of its therapeutic properties has been demonstrated to treat musculoskeletal conditions. However, this technology is not new, and has already been used in different experimental models and clinical studies for the treatment of tendinopathies, osteoarthritis, increased cell proliferation, bone consolidation, among others. Methods: In this work, we carried out an integrative review of clinical and experimental studies published in the last twenty years, on the available scientific evidence that demonstrate the effects of PEMF in different applications for health treatments. Five databases including Medline, Pubmed Central, Scopus, Lilacs and PEDro were searched for studies from 2001 to September 2022. The results were analyzed by the team of researchers and clinical professionals to assure methodological quality of the studies for the elaboration of the theoretical review on the effects of electromagnetic field stimulation on skeletal muscles, tendon or bones. Results: Sixty-two studies were included in this review, presenting evidence of the biological effects of PEMF that can suggest it´s possible use to treat different disorders. Conclusions: Pulsed electromagnetic fields (PEMF) present relevant clinical and experimental evidence of beneficial effects in the treatment of several musculoskeletal inflammatory disorders, such as tendinopathies and osteoarthritis, in addition to the treatment of urinary incontinence and abdominal diastasis. PEMF can be considered as the evolution of electrical currents for the treatment of musculoskeletal disorders, mainly due to its better tolerance by patients.

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Enhancing cartilage repair with optimized supramolecular hydrogel-based scaffold and pulsed electromagnetic field

Cited by 18 publications

References 57 publications

Injectable Bioadhesive Photocrosslinkable Hydrogels with Sustained Release of Kartogenin to Promote Chondrogenic Differentiation and Partial‐Thickness Cartilage Defects Repair

Injectable Bioadhesive Photocrosslinkable Hydrogels with Sustained Release of Kartogenin to Promote Chondrogenic Differentiation and Partial‐Thickness Cartilage Defects Repair

O-alg-THAM/gel hydrogels functionalized with engineered microspheres based on mesenchymal stem cell secretion recruit endogenous stem cells for cartilage repair

Applications of Pulsed Electromagnetic Field Therapy in Skeletal-Muscle System: An Integrative Review

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