Enhancing boll protein synthesis and carbohydrate conversion by the application of exogenous amino acids at the peak flowering stage increased the boll Bt toxin concentration and lint yield in cotton

LIU, Zhen-yu; LI, Yi-yang; Tambel, Leila I.M.; LIU, Yu-ting; Dai, Yuyang; Xu, Ze; LENG, Xin-hua; Zhang, Xiang; CHEN, De-hua; Yuan, Chen

doi:10.1016/j.jia.2022.10.003

Cited by 6 publications

(3 citation statements)

References 36 publications

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“…Chen et al [14,32,[37][38][39][40][41] have previously reported on the strong link between the expression of insecticidal proteins and nitrogen metabolism in cotton. This study aims to investigate the effect of low-temperature stress on various physiological parameters of the boll shell in Bt cotton.…”

Section: The Influence Of Low Temperature On Nitrogen Metabolism Resu...mentioning

confidence: 99%

“…Due to the unstable expression of Bt cotton insect resistance, the insecticidal effect is only about 40%, so related pests such as bollworm are still the main pests affecting cotton production [11,12]. The main reason for this is that the expression of Bt insecticidal protein (Bt protein) is characterized by spatiotemporal distribution [11,13,14]. In terms of time, the amount of Bt protein in the seedling bud stage is high, and it weakens after flowering.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism

Liu

et al. 2023

Plants

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Low temperature is the main factor for global natural disasters affecting the growth and distribution of plants, and cotton may be affected by low temperature and cold damage at all growth stages. In addition, the insecticidal resistance of cultivars has been reported to perform poorly or unstably due to adverse environments. The present study aimed to investigate the impact of low temperature on the levels of insecticidal protein in Bacillus thuringiensis (Bt) transgenic cotton plants during the peak boll stage. To achieve this, two Bt cotton cultivars, Sikang1 (SK1) and Sikang3 (SK3), were subjected to different temperature regimes and durations. The findings of the study demonstrated that the expression of insecticidal protein in the boll shell of Bt transgenic cotton plants was significantly inhibited under low-temperature stress. Specifically, in 2020, compared to the CK (27 °C), the insecticidal protein content in the boll shell of SK3 decreased by 28.19% after a 48 h of a 16 °C temperature. These results suggest that low-temperature stress can negatively impact the expression of insecticidal protein in Bt transgenic cotton, highlighting the need for appropriate measures to minimize its adverse effects on cotton production. In addition, the threshold temperature that leads to a significant decrease in the content of insecticidal proteins symbolizes an upward trend as the duration of stress prolongs. Decreased Bt protein content at low temperatures is associated with changes in the N metabolism. The present study revealed a significant positive correlation between the levels of glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities, as well as in the soluble protein levels in the boll shell and the content of the Bt protein. On the other hand, a significant negative correlation was observed between the levels of free amino acids, peptidase, and protease activities, as well as of Bt protein content. These findings suggest that, in Bt cotton production, it is crucial to remain vigilant of prolonged low-temperature disasters, which last for over 12 h and drop below 17–20 °C during the peak boll stage. Such conditions may reduce insecticidal resistance, leading to substantial economic losses.

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Section: The Influence Of Low Temperature On Nitrogen Metabolism Resu...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism

Liu

et al. 2023

Plants

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“…Despite the widespread cultivation of Bt cotton in China, the instability in the expression of Bt cotton's insecticidal protein has emerged as a pressing concern in the current landscape of transgenic cotton in China [9,10]. Cry1Ac endotoxin content gradually decreases throughout the growing season, reaching its lowest concentration during the boll-setting stage [11][12][13]. Furthermore, Cry1Ac endotoxin content varies across different cotton organs, with higher concentrations in the leaves and lower levels in reproductive organs like squares, owers, and bolls [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

Chen,

Dong,

Dai

et al. 2024

Preprint

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Background Sudden temperature drops, resulting from extreme weather events, often occur during the boll-setting period of cotton in Xinjiang, China, causing decreased expression of Bacillus thuringiensis (Bt) insecticidal proteins in cotton bolls. The precise threshold temperatures and durations that lead to significant changes in Cry1Ac endotoxin levels under low temperatures remain unclear. To address this, we investigated the effects of different temperatures and stress durations on Cry1Ac endotoxin levels in cotton bolls. In 2020–2021, two Bt transgenic cotton cultivars, conventional Sikang1 and hybrid Sikang3, were selected as experimental materials. Various low temperatures (ranging from 16 to 20°C) with different durations (12h, 24h and 48h) were applied during the peak boll-setting period. Results As the temperature decreased, the Cry1Ac endotoxin content in the boll shell, fiber, and seed exhibited a declining trend. Moreover, the temperature causing a significant change in Cry1Ac endotoxin content increased with the prolonged duration of low-temperature stress. Among the components of cotton bolls, seeds were most affected by low-temperature stress, with the threshold temperature for a significant reduction in Cry1Ac endotoxin content ranging from 17°C to 19°C. Correlation analysis indicated that low temperatures led to a decrease in protein synthesis capacity and an increase in degradation ability, resulting in reduced Cry1Ac endotoxin content. Pathway analysis revealed that both amino acid and peptidase had significant negative effects on Cry1Ac endotoxin content. Conclusion In summary, when the daily average temperature was ≤ 19°C, implementing cultural practices to reduce free amino acid content and peptidase activity could serve as effective cold defense strategies for Bt cotton production.

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Inhibition of protein degradation increases the Bt protein concentration in Bt cotton

LIU

Yuan

et al. 2024

Journal of Integrative Agriculture

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Enhancing boll protein synthesis and carbohydrate conversion by the application of exogenous amino acids at the peak flowering stage increased the boll Bt toxin concentration and lint yield in cotton

Cited by 6 publications

References 36 publications

Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism

Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

Inhibition of protein degradation increases the Bt protein concentration in Bt cotton

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