Enhancing blast disease resistance by overexpression of the calcium‐dependent protein kinase <i>Os<scp>CPK</scp>4</i> in rice

Bundó, Mireia; Coca, María

doi:10.1111/pbi.12500

Cited by 76 publications

(44 citation statements)

References 82 publications

(103 reference statements)

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“…The OsCPK10 (Fu et al, 2013) and OsCPK18 (Xie et al, 2014) were described as positive and negative regulators of M. oryzae resistance, respectively. Overexpression of OsCPK4 gene enhances resistance to rice blast disease and the constitutive accumulation of OsCPK4 protein prepares rice plants for a rapid and potentiated defense response (Bundó and Coca, 2016). The CPK and cyclic-nucleotide gated channels genes (LOC_Os10g28240.1, LOC_Os02g50174.1, LOC_Os11g44680.1, and LOC_Os11g44680.1) were up-regulated as early as 0 hpi until later stages in the Pi21 -RNAi line.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Highlights Defense and Signaling Pathways Mediated by Rice pi21 Gene with Partial Resistance to Magnaporthe oryzae

Zhao

Yuan

et al. 2016

Front. Plant Sci.

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Rice blast disease is one of the most destructive rice diseases worldwide. The pi21 gene confers partial and durable resistance to Magnaporthe oryzae. However, little is known regarding the molecular mechanisms of resistance mediated by the loss-of-function of Pi21. In this study, comparative transcriptome profiling of the Pi21-RNAi transgenic rice line and Nipponbare with M. oryzae infection at different time points (0, 12, 24, 48, and 72 hpi) were investigated using RNA sequencing. The results generated 43,222 unique genes mapped to the rice genome. In total, 1109 differentially expressed genes (DEGs) were identified between the Pi21-RNAi line and Nipponbare with M. oryzae infection, with 103, 281, 209, 69, and 678 DEGs at 0, 12, 24, 48, and 72 hpi, respectively. Functional analysis showed that most of the DEGs were involved in metabolism, transport, signaling, and defense. Among the genes assigned to plant—pathogen interaction, we identified 43 receptor kinase genes associated with pathogen-associated molecular pattern recognition and calcium ion influx. The expression levels of brassinolide-insensitive 1, flagellin sensitive 2, and elongation factor Tu receptor, ethylene (ET) biosynthesis and signaling genes, were higher in the Pi21-RNAi line than Nipponbare. This suggested that there was a more robust PTI response in Pi21-RNAi plants and that ET signaling was important to rice blast resistance. We also identified 53 transcription factor genes, including WRKY, NAC, DOF, and ERF families that show differential expression between the two genotypes. This study highlights possible candidate genes that may serve a function in the partial rice blast resistance mediated by the loss-of-function of Pi21 and increase our understanding of the molecular mechanisms involved in partial resistance against M. oryzae.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Highlights Defense and Signaling Pathways Mediated by Rice pi21 Gene with Partial Resistance to Magnaporthe oryzae

Zhao

Yuan

et al. 2016

Front. Plant Sci.

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“…In fact, SAG in rice can induce resistance as SA does (Bundó and Coca, ). In rice, SA‐mediated resistance is activated not only by SA but also by SAG, and SAG is differentially induced, suggesting that SAG is a key factor for SA‐mediated rice resistance rather than SA per se (Bundó and Coca, ). We therefore presume that the role of SAG in rice is unique compared to other plants.…”

Section: Discussionmentioning

confidence: 99%

“…Umemura et al () previously documented that RNAi suppression of OsSGT1 , a probenazole‐responsive UDP‐glucose:SA glucosyltransferase of rice, impaired probenazole‐dependent disease resistance against blast disease in rice plants, indicating that the role of the ratio of SA/SAG in rice seems to be different from that in other plants. In fact, SAG in rice can induce resistance as SA does (Bundó and Coca, ). In rice, SA‐mediated resistance is activated not only by SA but also by SAG, and SAG is differentially induced, suggesting that SAG is a key factor for SA‐mediated rice resistance rather than SA per se (Bundó and Coca, ).…”

Section: Discussionmentioning

confidence: 99%

Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness

Kobayashi

Fukuzawa

Hyodo

et al. 2020

Molecular Plant Pathology

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Salicylic acid (SA), an essential secondary messenger for plant defence responses, plays a role in maintaining a balance (trade‐off) between plant growth and resistance induction, but the detailed mechanism has not been explored. Because the SA mimic benzothiadiazole (BTH) is a more stable inducer of plant defence than SA after exogenous application, we analysed expression profiles of defence genes after BTH treatment to better understand SA‐mediated immune induction. Transcript levels of the salicylic acid glucosyltransferase (SAGT) gene were significantly lower in BTH‐treated Nicotiana tabacum (Nt) plants than in SA‐treated Nt control plants, suggesting that SAGT may play an important role in SA‐related host defence responses. Treatment with BTH followed by SA suppressed SAGT transcription, indicating that the inhibitory effect of BTH is not reversible. In addition, in BTH‐treated Nt and Nicotiana benthamiana (Nb) plants, an early high accumulation of SA and SA 2‐O‐β‐d‐glucoside was only transient compared to the control. This observation agreed well with the finding that SAGT‐overexpressing (OE) Nb lines contained less SA and jasmonic acid (JA) than in the Nb plants. When inoculated with a virus, the OE Nb plants showed more severe symptoms and accumulated higher levels of virus, while resistance increased in SAGT‐silenced (IR) Nb plants. In addition, the IR plants restricted bacterial spread to the inoculated leaves. After the BTH treatment, OE Nb plants were slightly larger than the Nb plants. These results together indicate that SAGT has a pivotal role in the balance between plant growth and SA/JA‐mediated defence for optimum plant fitness.

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“…Such defenses include generation of reactive oxygen species, callose deposition, synthesis of pathogenesis-related (PR) proteins, and increased activation of systemic acquired resistance (SAR) [23,61]. As with the previously described strategies, this strategy takes advantage of the plant's own natural immune system and does not introduce new metabolic pathways.…”

Section: Upregulating Defense Pathwaysmentioning

confidence: 99%

“…This approach has been successful against bacterial pathogens attacking several host species [62][63][64], and it offers promising results for enhancing resistance to citrus greening [23], a disease of urgency for the citrus industry. Upregulation of defense pathways was also successful against destructive fungal pathogens, including Rhizoctonia solani (the cause of many diseases) and Magnaporthe oryzae (the cause of rice blast) [61,65]. In both cases, resistance was achieved by expressing a native rice gene under the control of a constitutive promotor from maize, introducing neither a novel pathway nor a non-crop gene.…”

Section: Upregulating Defense Pathwaysmentioning

confidence: 99%

Genetic Engineering and Sustainable Crop Disease Management: Opportunities for Case-by-Case Decision-Making

Vincelli

2016

Sustainability

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Genetic engineering (GE) offers an expanding array of strategies for enhancing disease resistance of crop plants in sustainable ways, including the potential for reduced pesticide usage. Certain GE applications involve transgenesis, in some cases creating a metabolic pathway novel to the GE crop. In other cases, only cisgenessis is employed. In yet other cases, engineered genetic changes can be so minimal as to be indistinguishable from natural mutations. Thus, GE crops vary substantially and should be evaluated for risks, benefits, and social considerations on a case-by-case basis. Deployment of GE traits should be with an eye towards long-term sustainability; several options are discussed. Selected risks and concerns of GE are also considered, along with genome editing, a technology that greatly expands the capacity of molecular biologists to make more precise and targeted genetic edits. While GE is merely a suite of tools to supplement other breeding techniques, if wisely used, certain GE tools and applications can contribute to sustainability goals.

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Enhancing blast disease resistance by overexpression of the calcium‐dependent protein kinase OsCPK4 in rice

Cited by 76 publications

References 82 publications

Transcriptome Analysis Highlights Defense and Signaling Pathways Mediated by Rice pi21 Gene with Partial Resistance to Magnaporthe oryzae

Transcriptome Analysis Highlights Defense and Signaling Pathways Mediated by Rice pi21 Gene with Partial Resistance to Magnaporthe oryzae

Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness

Genetic Engineering and Sustainable Crop Disease Management: Opportunities for Case-by-Case Decision-Making

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