Enhancing Biopolymer Hydrogel Functionality through Interpenetrating Networks

Dhand, Abhishek; Galarraga, Jonathan H.; Burdick, Jason A.

doi:10.1016/j.tibtech.2020.08.007

Cited by 158 publications

(118 citation statements)

References 142 publications

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“…1 Hydrogels remain one of the most promising matrices to imitate the ECM of soft tissues due to their hydrated macromolecular network structure. 2,3 Already by using synthetically tailorable hydrogels, the effects of stiffness, [4][5][6] stress relaxation, [7][8][9] and 3D shape 10 on cell behavior and tissue formation have been elucidated; via biofabrication technologies, these hydrogels can also enable the creation of anatomically correct structures. 11 While the majority of hydrogels used to study cellmatrix interactions and fabricate 3D tissue constructs consist of single network hydrogels, double network (DN) hydrogels are appealing candidates for recapitulating properties of the native ECM.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic double network hydrogels: Combining dynamic and static crosslinks to enable biofabrication and control cell‐matrix interactions

Aldana

Morgan

Houben

et al. 2021

Journal of Polymer Science

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Hydrogels are promising candidates for recapitulation of the native extracellular matrix (ECM), yet recreating molecular and spatiotemporal complexity within a single network remains a challenge. Double network (DN) hydrogels are a promising step towards recapitulating the multicomponent ECM and have enhanced mechanical properties. Here, we investigate DNs based on dynamic covalent and covalent bonds to mimic the dynamicity of the ECM and enable biofabrication. We also investigate the spatiotemporal molecular attachment of a bioactive adhesive peptide within the networks. Using oxidized alginate (dynamic network, Schiff base) and polyethylene glycol diacrylate (static network, acrylate polymerization) we find an optimized procedure, where the dynamic network is formed first, followed by the static network. This initial dynamically cross-linked hydrogel imparts self-healing, injectability, and 3D printability, while the subsequent DN hydrogel improves the stability of the 3D gels and imparts toughness. Rheology and compression testing show that the toughening is due to the combination of energy dissipation (dynamic network) and elasticity (static network). Furthermore, where we place adhesive sites in the network matters; we find distinct differences when an adhesive peptide, Arg-Gly-Asp (RGD), is attached to the different networks. This DN strategy bring us closer to understanding and recreating the complex multicomponent ECM-pushing us past a materials view of cell adhesionwhile enabling injectabiltiy and printing of tough hydrogels.

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Section: Introductionmentioning

confidence: 99%

Biomimetic double network hydrogels: Combining dynamic and static crosslinks to enable biofabrication and control cell‐matrix interactions

Aldana

Morgan

Houben

et al. 2021

Journal of Polymer Science

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“…Despite these differences, in both cases, it is crucial to possess information on the basic functional features of the hydrogels used and the relationships between them. It should be noted that the properties of these materials can be modified to some extent, predominantly by changing their chemical composition [ 29 , 30 , 31 ]. Due to the use of different proportions of two or more polymeric building blocks, it is possible to influence the sorption properties (e.g., the swelling ability, the water absorption capacity, and hydrolytic degradation) as well as the mechanical resistance or sensitivity to external stimuli of the resulting material.…”

Section: Introductionmentioning

confidence: 99%

Functional Properties of Two-Component Hydrogel Systems Based on Gelatin and Polyvinyl Alcohol—Experimental Studies Supported by Computational Analysis

Labus

Radosiński

Kotowski

2021

IJMS

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The presented research is focused on an investigation of the effect of the addition of polyvinyl alcohol (PVA) to a gelatin-based hydrogel on the functional properties of the resulting material. The main purpose was to experimentally determine and compare the properties of hydrogels differing from the content of PVA in the blend. Subsequently, the utility of these matrices for the production of an immobilized invertase preparation with improved operational stability was examined. We also propose a useful computational tool to predict the properties of the final material depending on the proportions of both components in order to design the feature range of the hydrogel blend desired for a strictly specified immobilization system (of enzyme/carrier type). Based on experimental research, it was found that an increase in the PVA content in gelatin hydrogels contributes to obtaining materials with a visibly higher packaging density, degree of swelling, and water absorption capacity. In the case of hydrolytic degradation and compressive strength, the opposite tendency was observed. The functionality studies of gelatin and gelatin/PVA hydrogels for enzyme immobilization indicate the very promising potential of invertase entrapped in a gelatin/PVA hydrogel matrix as a stable biocatalyst for industrial use. The molecular modeling analysis performed in this work provides qualitative information about the tendencies of the macroscopic parameters observed with the increase in the PVA and insight into the chemical nature of these dependencies.

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“…As expected, after adding fibrin, the matrix formed a crosslinked interpenetrating polymer network (IPN). [41] To ensure the appropriate biocompatibility, 3% GelMA (3G) and 5% GelMA (5G) were selected as the base, and different concentrations of fibrin solutions were introduced into this base solution. The SEM micrographs revealed that the average size of interconnected pores increased with lowering of the GelMA concentration (Figure 3C).…”

Section: Resultsmentioning

confidence: 99%

A novel method for generating 3D constructs with branched vascular networks using multi-materials bioprinting and direct surgical anastomosis

Liu

Wang

Zhang

et al. 2021

Preprint

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Vessels pervade almost all body tissues, and significantly influence the pathophysiology of human body. Previous attempts to establish multi-scale vascular connection and function in 3D model tissues using bioprinting have had limited success due to the incoordination between cell-laden materials and stability of the perfusion channel. Here, we report a methodology to fabricate centimetre-scale vascularized soft tissue with high viability and accuracy using multi-materials bioprinting involving inks with low viscosity and a customized multistage-temperature-control printer. The tissue formed was perfused with branched vasculature with well-formed 3D capillary network and lumen, which would potentially supply the cellular components with sufficient nutrients in the matrix. Furthermore, the same methodology was applied for generating liver-like tissue with the objective to fabricate and mimic a mature and functional liver tissue, with increased functionality in terms of synthesis of liver specific proteins after in vitro perfusion and in vivo subperitoneal transplantation in mice. Moreover, to establish immediate blood perfusion, an elastic layer was printed wrapping sacrificial ink to support the direct surgical anastomosis of the carotid artery to the jugular vein. Our findings highlight the support extended by vasculature network in soft hydrogels which helps to sustain the thick and dense cellularization in engineered tissues.

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Enhancing Biopolymer Hydrogel Functionality through Interpenetrating Networks

Cited by 158 publications

References 142 publications

Biomimetic double network hydrogels: Combining dynamic and static crosslinks to enable biofabrication and control cell‐matrix interactions

Biomimetic double network hydrogels: Combining dynamic and static crosslinks to enable biofabrication and control cell‐matrix interactions

Functional Properties of Two-Component Hydrogel Systems Based on Gelatin and Polyvinyl Alcohol—Experimental Studies Supported by Computational Analysis

A novel method for generating 3D constructs with branched vascular networks using multi-materials bioprinting and direct surgical anastomosis

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