Enhancing Biomass and Lutein Production From Scenedesmus almeriensis: Effect of Carbon Dioxide Concentration and Culture Medium Reuse

Molino, Antonio; Mehariya, Sanjeet; Iovine, Angela; Casella, Patrizia; Marino, Tiziana; Karatza, Despina; Chianese, Simeone; Musmarra, Dino

doi:10.3389/fpls.2020.00415

Cited by 55 publications

(19 citation statements)

References 54 publications

(72 reference statements)

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“…S. almeriensis is a good candidate for commercial applications since it exhibits significant protein content (50-55% of dry weight) with satisfactory growth rates in a broad range of environmental conditions [46] and is already studied in medium scale production [47]. This microalga has been mainly studied for lutein production and extraction [48,49]. Bauer et al performed lipid extraction from S. almeriensis among other microalgae with liquefied dimethyl ether although the authors utilized freeze-dried biomass in their study.…”

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confidence: 99%

Analysis of the lipid extraction performance in a cascade process for Scenedesmus almeriensis biorefinery

Papachristou

Akaberi

Silve

et al. 2021

Biotechnol Biofuels

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Background Microalgae have attracted considerable interest due to their ability to produce a wide range of valuable compounds. Pulsed Electric Fields (PEF) has been demonstrated to effectively disrupt the microalgae cells and facilitate intracellular extraction. To increase the commercial viability of microalgae, the entire biomass should be exploited with different products extracted and valorized according to the biorefinery scheme. However, demonstrations of multiple component extraction in series are very limited in literature. This study aimed to develop an effective lipid extraction protocol from wet Scenedesmus almeriensis after PEF-treatment with 1.5 MJ·kgDW−1. A cascade process, i.e., the valorization of several products in row, was tested with firstly the collection of the released carbohydrates in the water fraction, then protein enzymatic hydrolysis and finally lipid extraction. Biomass processed with high pressure homogenization (HPH) on parallel, served as benchmark. Results Lipid extraction with ethanol:hexane (1:0.41 vol/vol) offered the highest yields from the different protocols tested. PEF-treatment promoted extraction with almost 70% of total lipids extracted against 43% from untreated biomass. An incubation step after PEF-treatment, further improved the yields, up to 83% of total lipids. Increasing the solvent volume by factor 2 offered no improvement. In comparison, extraction with two other systems utilizing only ethanol at room temperature or elevated at 60 °C were ineffective with less than 30% of total lipids extracted. Regarding cascade extraction, carbohydrate release after PEF was detected albeit in low concentrations. PEF-treated samples displayed slightly better kinetics during the enzymatic protein hydrolysis compared to untreated or HPH-treated biomass. The yields from a subsequent lipid extraction were not affected after PEF but were significantly increased for untreated samples (66% of total lipids), while HPH displayed the lowest yields (~ 49% of total lipids). Conclusions PEF-treatment successfully promoted lipid extraction from S. almeriensis but only in combination with a polar:neutral co-solvent (ethanol:hexane). After enzymatic protein hydrolysis in cascade processing; however, untreated biomass displayed equal lipid yields due to the disruptive effect of the proteolytic enzymes. Therefore, the positive impact of PEF in this scheme is limited on the improved reaction kinetics exhibited during the enzymatic hydrolysis step.

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confidence: 99%

Analysis of the lipid extraction performance in a cascade process for Scenedesmus almeriensis biorefinery

Papachristou

Akaberi

Silve

et al. 2021

Biotechnol Biofuels

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“…The diameter of the lighting system for PBR with V/S of 56.5 L/m 2 was equal to 350 mm, while the diameter of the lighting system for PBR with V/S of 11.5 L/m 2 was 160 mm and lighting systems are controlled and regulated by SCADA (Supervisory Control and Data Acquisition). Temperature and pH were monitored in real time by SCADA in a user interface consisting of a custom software and PC with touchscreen [ 48 , 49 ].…”

Section: Methodsmentioning

confidence: 99%

An Integrated Strategy for Nutraceuticals from Haematoccus pluvialis: From Cultivation to Extraction

et al. 2020

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The aim of this study was to develop an effective integrated cultivation system for Haematococcus pluvialis as a source of bioactive compounds such as astaxanthin, lutein, proteins, and fatty acids (FAs). The Chlorophyta H. pluvialis was cultivated in a vertical bubble column photobioreactor (VBC-PBR) under batch mode, allowing switching from green to red phase for astaxanthin induction. The combined effect of light intensity and nutrients on bioactive compound formation was investigated. Results showed that growth under lower nutrients availability and light intensity led to a higher concentration of biomass. Growth under high light intensity with an appropriate concentration of nitrate, sulfate, phosphate and magnesium led to ~85% and ~58% higher production of total carotenoids and fatty acids, respectively. Under high stress conditions, ~90% nitrate and phosphate consumption were observed.

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“…Higher DOC (0.28–1.94 mg/L) with subsequent number of recycle was believed to promote the mixotrophic growth (Schädler et al, 2020). Scenedesmus almeriensis growth was inhibited in recycled water at low CO 2 supply and limited availability of nutrients (Molino et al, 2020). Recycling of water after harvesting by water‐soluble flocculants depends on the residual concentration of flocculants in recycled water.…”

Section: Cultivation Of Microalgae In Recycled Watermentioning

confidence: 99%

Sustainable production of microalgae biomass for biofuel and chemicals through recycling of water and nutrient within the biorefinery context: A review

Farooq

2021

GCB Bioenergy

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Commercial‐scale production of microalgae biomass for biofuel and biochemicals requires a substantial amount of water and nutrients. Many studies are conducted to explore the potential of various aqueous streams originating from harvesting stage and different energy recovery steps as an alternative for water and nutrient supply. Presence of toxic organic compounds, unassimilated ions, particulate matter, and high alkalinity in post‐harvest water limit its recyclability. Nutrient recovery from biomass via anaerobic digestion (AD) and various hydrothermal processes is being explored. So, there is a need to understand the impact of harvesting methods, nature and impact of organic compounds, buildup of algogenic organic matter (OM), amount of unused nutrients, and salinity on water recycling. Optimum conditions for maximum nutrient recovery from AD and hydrothermal processes are discussed for effective nutrient recycling. This review is an attempt to understand the challenges associated with the recycling of aqueous streams for water and nutrient requirement for sustainable microalgae cultivation. The effectiveness of a recycling stream is defined here as “biomass ratio.” Possible growth inhibiting factors are identified, and their solutions are suggested along with potential directions for future research. Large‐scale sustainable cultivation of microalgae through recycling of different streams depends on better understanding of biological activity of algal OM through detailed characterization and in‐depth understanding of physiochemical properties.

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Enhancing Biomass and Lutein Production From Scenedesmus almeriensis: Effect of Carbon Dioxide Concentration and Culture Medium Reuse

Cited by 55 publications

References 54 publications

Analysis of the lipid extraction performance in a cascade process for Scenedesmus almeriensis biorefinery

Analysis of the lipid extraction performance in a cascade process for Scenedesmus almeriensis biorefinery

An Integrated Strategy for Nutraceuticals from Haematoccus pluvialis: From Cultivation to Extraction

Sustainable production of microalgae biomass for biofuel and chemicals through recycling of water and nutrient within the biorefinery context: A review

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