Enhancing bacterial fitness and recombinant enzyme yield by engineering the quality control protease HtrA of
Bacillus subtilis
Ayşegül Öktem,
David Núñez-Nepomuceno,
Borja Ferrero-Bordera
et al.
Abstract:Bacillus subtilis
is a Gram-positive bacterium that is well-known for its high secretory capacity. Although secretion of homologous proteins is extremely efficient, secretion of heterologous proteins imposes what is known as “secretion stress” on the cells. This is not desirable in an industrial setting, because the bacterial secretion stress responses can adversely affect product yields. So far, the main approach to reduce such counterproductive secretion stress responses has been to remove compon… Show more
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