Enhancing Astaxanthin Biosynthesis by Rhodosporidium toruloides Mutants and Optimization of Medium Compositions Using Response Surface Methodology

Abstract

Astaxanthin is a valuable carotenoid, which has been approved as a food coloring by the US Food and Drug Administration and is considered as a food dye by the European Union (European Commission). This work aimed to attain Rhodosporidium toruloides mutants for enhanced astaxanthin accumulation using ultraviolet (UV) and gamma irradiation mutagenesis. Gamma irradiation was shown to be more efficient than UV for producing astaxanthin-overproducer. Among the screened mutants, G17, a gamma-induced mutant, exhibited the highest astaxanthin production, which was significantly higher than that of the wild strain. Response surface methodology was then applied to optimize the medium compositions for maximizing astaxanthin production by the mutant G17. The optimal medium compositions for the cultivation of G17 were determined as a peptone concentration of 19.75 g/L, malt extract concentration of 13.56 g/L, and glucose concentration of 19.92 g/L, with the maximum astaxanthin yield of 3021.34 µg/L ± 16.49 µg/L. This study suggests that the R. toruloides mutant (G17) is a potential candidate for astaxanthin production.

Emerging Technologies for Biorefining, Food and Environmental Applications

“…The contributors of this Special Issue are also aware of the production of new materials and feedstock for promising applications. This is the case of the report provided by Tran et al [10], who enhanced the astaxanthin biosynthesis by means of Rhodosporidium toruloides mutants. As it is well known, astaxanthin is a high-added-value compound that is potentially suggested in new food formulations as a natural colorant.…”

Generative Artificial Intelligence GPT-4 Accelerates Knowledge Mining and Machine Learning for Synthetic Biology

“…For instance, reports on astaxanthin production in R. toruloides mainly focused on its native pathways. − Therefore, knowledge transferring from Y. lipolytica reports is necessary to predict how genetic engineering affects astaxanthin production in R.…”

Mutagenesis of Rhodosporidium toruloides using ethyl methanesulfonate for enhancing astaxanthin production and evaluation scavenging activity against 2,2-diphenyl-1-picrylhydrazyl of astaxanthin

“…Tỉ lệ tế bào nấm men R. toruloides sống sót được tính theo công thức (1): Tỉ lệ sống sót = (Cs/Cc) x100 (1) Trong đó Cs và Cc tương ứng là tổng số tế bào đếm được sau khi xử lý với tác nhân gây đột biến và mẫu đối chứng (mẫu không xử lý với tác nhân gây đột biến) 13…”