Enhancing Agrobacterium-mediated plant transformation efficiency through improved ternary vector systems and auxotrophic strains
Ephraim Aliu,
Qing Ji,
Anna Wlazlo
et al.
Abstract:Agrobacterium-mediated transformation is an essential tool for functional genomics studies and crop improvements. Recently developed ternary vector systems, which consist of a T-DNA vector and a compatible virulence (vir) gene helper plasmid (ternary helper), demonstrated that including an additional vir gene helper plasmid into disarmed Agrobacterium strains significantly improves T-DNA delivery efficiency, enhancing plant transformation. Here, we report the development of a new ternary helper and thymidine a… Show more
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