Enhancer of splitD, a dominant mutation of Drosophila, and its use in the study of functional domains of a helix-loop-helix protein.

Tietze, Kyria; Oellers, Nadja; Knust, Elisabeth

doi:10.1073/pnas.89.13.6152

Cited by 115 publications

(146 citation statements)

References 39 publications

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“…S1 A). This site is an 8 bp palindrome, including an inverted 6 bp repeat of the class C, E-box binding site 5Ј-CACGCG-3Ј (30,31). Disruption of the proximal 5Ј-CACGCG-3Ј resulted in ubiquitous reporter expression ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Gene regulatory network subcircuit controlling a dynamic spatial pattern of signaling in the sea urchin embryo

Smith

Davidson

2008

Proc. Natl. Acad. Sci. U.S.A.

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We dissect the transcriptional regulatory relationships coordinating the dynamic expression patterns of two signaling genes, wnt8 and delta, which are central to specification of the sea urchin embryo endomesoderm. cis-Regulatory analysis shows that transcription of the gene encoding the Notch ligand Delta is activated by the widely expressed Runx transcription factor, but spatially restricted by HesC-mediated repression through a site in the delta 5 UTR. Spatial transcription of the hesC gene, however, is controlled by Blimp1 repression. Blimp1 thus represses the repressor of delta, thereby permitting its transcription. The blimp1 gene is itself linked into a feedback circuit that includes the wnt8 signaling ligand gene, and we showed earlier that this circuit generates an expanding torus of blimp1 and wnt8 expression. The finding that delta expression is also controlled at the cis-regulatory level by the blimp1-wnt8 torus-generating subcircuit now explains the progression of Notch signaling from the mesoderm to the endoderm of the developing embryo. Thus the specific cis-regulatory linkages of the gene regulatory network encode the coordinated spatial expression of Wnt and Notch signaling as they sweep outward across the vegetal plate of the embryo.Blimp1 ͉ Delta ͉ HesC ͉ Notch ͉ Wnt

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Section: Resultsmentioning

confidence: 99%

Gene regulatory network subcircuit controlling a dynamic spatial pattern of signaling in the sea urchin embryo

Smith

Davidson

2008

Proc. Natl. Acad. Sci. U.S.A.

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“…The E(spl) locus encodes 7 small basic-helix-loop-helix (bHLH) transcriptional repressor proteins and Groucho (Delidakis et al, 199 1;Delidakis et al, 1992;Knust et al, 1992). E(Spl)bHLHE(spl)Groucho complexes act as transcriptional repressors (Tietze et al, 1992). A putative binding site for Su(H) has been found in regulatory sequences of the E(sp1) genes, and the transcription of this locus has been found to require the Su(H) protein (Tun et al, 1994;Henkel et al, 1994;Grossrnan et al, 1994;Zimmer-Strobl et al, 1994).…”

Section: The Notch Signal Transduction Pathwavmentioning

confidence: 99%

Expression of notch receptors, notch ligands, and fringe genes in hematopoiesis

Singh¹,

Phillips

Iscove

et al. 2000

Experimental Hematology

101

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“…Recently, Dpn also has been found to bind DNA (K. Wallace and H. Vaessin, in prep.). Likewise, E(spl)m8, another protein with a bHLH region related to Hairy, binds DNA and this activity is required to mediate the E(spl)" phenotype (Tietze et al 1992). Sna has been shown to function as a repressor over short distances (< 100-150 bp; Ip et al 1992;Gray et al 1994).…”

Section: The Role Of Known Pan-neural Genes In Neurogenesismentioning

confidence: 99%

scratch, a pan-neural gene encoding a zinc finger protein related to snail, promotes neuronal development.

Roark

Sturtevant²,

Emery³

et al. 1995

Genes Dev.

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The Drosophila scratch {scrt) gene is expressed in most or all neuronal precursor cells and encodes a predicted zinc finger transcription factor closely related to the product of the mesoderm determination gene snail {sna). Adult flies homozygous for scrt null alleles have a reduced number of photoreceptors in the eye, and embryos lacking the function of both scrt and the pan-neural gene deadpan [dpn], which encodes a basic helix-loop-helix (bHLH) protein, exhibit a significant loss of neurons. Conversely, ectopic expression of a scrt transgene during embryonic and adult development leads to the production of supernumerary neurons. Consistent with scrt functioning as a transcription factor, various genes are more broadly expressed than normal in scrt null mutants. Reciprocally, these same genes are expressed at reduced levels in response to ectopic scrt expression. We propose that scrt promotes neuronal cell fates by suppressing expression of genes promoting non-neuronal cell fates. We discuss the similarities between the roles of the ancestrally related scrt, sna, and escargot [esc] genes in regulating cell fate choices.

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Enhancer of splitD, a dominant mutation of Drosophila, and its use in the study of functional domains of a helix-loop-helix protein.

Cited by 115 publications

References 39 publications

Gene regulatory network subcircuit controlling a dynamic spatial pattern of signaling in the sea urchin embryo

Gene regulatory network subcircuit controlling a dynamic spatial pattern of signaling in the sea urchin embryo

Expression of notch receptors, notch ligands, and fringe genes in hematopoiesis

scratch, a pan-neural gene encoding a zinc finger protein related to snail, promotes neuronal development.

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