Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts

Tamura, Toshiaki; Inoue, Takafumi; Nagata, Kyosuke; Mikoshiba, Katsuhiko

doi:10.1016/s0006-291x(88)80265-1

Cited by 46 publications

(35 citation statements)

References 19 publications

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“…5a, b). We found strong protections in the NFI motif and pseudo-NFI (~-NFI) motif ofpJC1B DNA as previously reported (Tamura et al, 1988). In this study, we found a further protected site on sequences just upstream from the NFI motif.…”

Section: A New Transcription Element In the Jc Virus Enhancersupporting

confidence: 90%

“…37 and 62 of JCV includes essential sequences for brain-specific viral gene expression. Several groups have demonstrated that a nuclear factor I (NFI) motif is present in this region and that the NFI motif actually functions during JCV DNA transcription (Tamura et al, 1988;Amemiya et al, 1989). However, transcription elements and their cognate factors in this region have not yet been thoroughly characterized.…”

Section: A New Transcription Element In the Jc Virus Enhancermentioning

confidence: 99%

“…)/HindlII (268 m.p.) fragment of JCV DNA carrying one enhancer unit was inserted into pBR322 to construct pJC1B, pJC1B was transcribed in mouse brain nuclear extract and specific transcripts from the viral early promoter were analysed by S1 nuclease mapping (Tamura et al, 1988(Tamura et al, , 1989 (Fig. 2).…”

Section: A New Transcription Element In the Jc Virus Enhancermentioning

confidence: 99%

“…2). Previous studies (Tamura et al, 1988) demonstrated that in vitro transcription of the two enhancer unit-carrying DNA was initiated from the wrong position (91 m.p.) for unknown reasons (Fig.…”

Section: A New Transcription Element In the Jc Virus Enhancermentioning

confidence: 99%

“…Similar experiments were carried out using pJC1BN DNA ( In vitro transcription from single enhancer unit-carrying JCV templates and effect of mutation. Each plasmid DNA (600 ng) was transcribed in brain nuclear extract or in HeLa whole cell extract for 45 min at 30 °C (Tamura et al, 1988(Tamura et al, , 1989) and the specific transcription signal was analysed by SI nuclear mapping. Probe DNA for JCV and MLP contained sequences from PvulI to Seal (pBR322) and those from EcoRI to Sall (pBR322), respectively.…”

Section: A New Transcription Element In the Jc Virus Enhancermentioning

confidence: 99%

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A New Transcription Element in the JC Virus Enhancer

Tamura

Aoyama

Inoue

et al. 1990

Journal of General Virology

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Section: A New Transcription Element In the Jc Virus Enhancersupporting

confidence: 90%

Section: A New Transcription Element In the Jc Virus Enhancermentioning

confidence: 99%

Section: A New Transcription Element In the Jc Virus Enhancermentioning

confidence: 99%

Section: A New Transcription Element In the Jc Virus Enhancermentioning

confidence: 99%

Section: A New Transcription Element In the Jc Virus Enhancermentioning

confidence: 99%

See 3 more Smart Citations

A New Transcription Element in the JC Virus Enhancer

Tamura

Aoyama

Inoue

et al. 1990

Journal of General Virology

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Lineage pathway of human brain progenitor cells identified by JC virus susceptability

Messam

Hou

Gronostajski

et al. 2003

Annals of Neurology

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Multipotential human central nervous system progenitor cells, isolated from human fetal brain tissue by selective growth conditions, were cultured as undifferentiated, attached cell layers. Selective differentiation yielded highly purified populations of neurons or astrocytes. This report describes the novel use of this cell culture model to study cell type-specific recognition of a human neurotropic virus, JC virus. Infection by either JC virions or a plasmid encoding the JC genome demonstrated susceptibility in astrocytes and, to a lesser degree, progenitor cells, whereas neurons remained nonpermissive. JC virus susceptibility correlated with significantly higher expression of the NFI-X transcription factor in astrocytes than in neurons. Furthermore, transfection of an NFI-X expression vector into progenitor-derived neuronal cells before infection resulted in viral protein production. These results indicate that susceptibility to JC virus infection occurs at the molecular level and also suggest that differential recognition of the viral promoter sequences can predict lineage pathways of multipotential progenitor cells in the human central nervous system.

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The transcription factor Sp1 binds to the JC virus promoter and is selectively expressed in glial cells in human brain

Henson

Saffer

Furneaux

1992

Annals of Neurology

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JC virus is a human DNA virus that specifically infects oligodendroglial cells, resulting in a demyelinating disease (progressive multifocal leukoencephalopathy) of the central nervous system of immunosuppressed patients. The host-range restriction of JC virus is controlled at the level of viral gene transcription. To analyze further the determinants of glial specificity, we cloned and sequenced the JC viral early promoter elements directly from the infected brain tissue of four patients. The promoter of each isolate contained a novel identical sequence, 5'-AGGGAGGAGC (GA box), located immediately upstream of the TATA box. This GA box is not present in the original isolate of JC virus (Mad-1 strain), which was obtained after numerous passages in tissue culture. The GA box has 80% homology with the consensus binding site for the transcription factor Sp1. Using a gel retardation assay, we found that Sp1 binds to the GA box. Alteration of bases within the sequence abolished binding of Sp1, demonstrating sequence specificity of binding. Immunohistochemical localization of Sp1 expression in human brain reveals that expression is restricted to the nuclei of oligodendroglial cells, cerebellar basket cells, and endothelial cells. The GA box is present in the promoters of the myelin basic protein and proteolipid protein genes. On the basis of these observations, we suggest that this Sp1-like binding site participates in the control of glial-specific gene expression.

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Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts

Cited by 46 publications

References 19 publications

A New Transcription Element in the JC Virus Enhancer

A New Transcription Element in the JC Virus Enhancer

Lineage pathway of human brain progenitor cells identified by JC virus susceptability

The transcription factor Sp1 binds to the JC virus promoter and is selectively expressed in glial cells in human brain

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