Enhancer-Driven Gene Expression (EDGE) enables the generation of cell type specific tools for the analysis of neural circuits

Blankvoort, Stefan; Descamps, Lucie A.L.; Kentros, Clifford

doi:10.1016/j.neures.2020.01.009

Cited by 17 publications

(8 citation statements)

References 73 publications

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“…Here, the combination of optogenetic stimulation or inactivation with fMRI holds great promise due to its wide areal coverage. Finally, optogenetic targeting, including its combination with fMRI, will benefit from the availability of more efficient and more selective neuronal transduction approaches (Bedbrook et al, 2019;Blankvoort et al, 2020;Deverman et al, 2016).…”

Section: Discussion and Outlookmentioning

confidence: 99%

Combining Brain Perturbation and Neuroimaging in Non-human Primates

Klink¹,

jean-francois.aubry@espci.fr²,

Ferrera³

et al. 2020

Preprint

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Brain perturbation studies allow detailed causal inferences of behavioral and neural processes. Because the combination of brain perturbation methods and neural measurement techniques is inherently challenging, research in humans has predominantly focused on non-invasive, indirect brain perturbations, or neurological lesions. Non-human primates have been indispensable as a neurobiological system that is highly similar to humans while simultaneously being more experimentally tractable, allowing visualization of the functional and structural impact of systematic brain perturbation. This review considers the state-of-the-art in non-human primate brain perturbation with a focus on approaches that can be combined with neuroimaging. We consider both non-reversible (lesions) and reversible or temporary perturbations such as electrical, pharmacological, optical, optogenetic, chemogenetic, pathway-selective, and ultrasound based interference methods. Method-specific considerations from the research and development community are offered to facilitate research in this field and support further innovations. We conclude by identifying novel avenues for further research and innovation and by highlighting the clinical translational potential of the methods.

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Section: Discussion and Outlookmentioning

confidence: 99%

Combining Brain Perturbation and Neuroimaging in Non-human Primates

Klink¹,

jean-francois.aubry@espci.fr²,

Ferrera³

et al. 2020

Preprint

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“…These experiments were approved by the Federation of European Laboratory Animal Science Association (FELASA) and local authorities at NTNU. CC-EDGE transgenic mice were provided by Dr. Stefan Blankvoort (79, 80), while TRE-tight-THAG transgenic mice were provided by Dr. Rajeevkumar R. Nair.…”

Section: Methodsmentioning

confidence: 99%

Single neurons and networks in the claustrum integrate input from widespread cortical sources

Shelton

Oliver

Grimstvedt

et al. 2022

Preprint

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The claustrum is highly interconnected with many structures in the brain, but the organizing principles governing its vast connectivity have yet to be fully explored. We investigated the defining characteristics and activity of single claustrum neurons, the nature of their relationship with the cortex, and their connectivity within the claustrum itself to gain a comprehensive view of claustral circuits. We show that the claustrum is composed of excitatory and inhibitory cell types that are connected through intraclaustral excitatory synapses, especially between neurons with disparate projection targets. Investigations of corticoclaustral innervation demonstrated that axons from the cortex localize to distinct dorsoventral modules depending on their region of origin. In vitro dual-color optogenetic mapping experiments revealed that individual claustrum neurons integrated inputs from more than one cortical region in a cell type- and projection target-specific manner. Integration in claustrum neurons was observed to be particularly common between areas of the frontal cortex and less so for sensory, motor, and association cortices. Finally, activity in claustrum axons in midline cortical areas recorded with in vivo two-photon calcium imaging showed responses to multimodal sensory stimuli. Our findings shed light on the organizing principles of claustrum circuits, demonstrating that individual claustrum neurons integrate cortical inputs and redistribute this information back to cortex after performing output target- and cell type-dependent local computations.

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“…For birth-dating of claustrum neurons, C57/Bl6 or MEC-13-53D mice ( Blankvoort et al. 2018 , 2020 ) crossed with homozygous Tg(tetO-GCaMP6s)2Niell (GCaMP6s) mice or Nkx2.1Cre;Z/EG mice crossed with homozygous B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (Ai9) mice were mated and plug-checked daily. Day of plug was designated as embryonic day (E) 0.5.…”

Section: Methodsmentioning

confidence: 99%

Temporal origin of mouse claustrum and development of its cortical projections

et al. 2022

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The claustrum is known for its extensive connectivity with many other forebrain regions, but its elongated shape and deep location have made further study difficult. We have sought to understand when mouse claustrum neurons are born, where they are located in developing brains, and when they develop their widespread connections to the cortex. We established that a well-characterized parvalbumin plexus, which identifies the claustrum in adults, is only present from postnatal day (P) 21. A myeloarchitectonic outline of the claustrum can be derived from a triangular fiber arrangement from P15. A dense patch of Nurr1+ cells is present at its core and is already evident at birth. Bromodeoxyuridine birth dating of forebrain progenitors reveals that the majority of claustrum neurons are born during a narrow time window centered on embryonic day 12.5, which is later than the adjacent subplate and endopiriform nucleus. Retrograde tracing revealed that claustrum projections to anterior cingulate (ACA) and retrosplenial cortex (RSP) follow distinct developmental trajectories. Claustrum–ACA connectivity matures rapidly and reaches adult-like innervation density by P10, whereas claustrum–RSP innervation emerges later over a protracted time window. This work establishes the timeline of claustrum development and provides a framework for understanding how the claustrum is built and develops its unique connectivity.

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Enhancer-Driven Gene Expression (EDGE) enables the generation of cell type specific tools for the analysis of neural circuits

Cited by 17 publications

References 73 publications

Combining Brain Perturbation and Neuroimaging in Non-human Primates

Combining Brain Perturbation and Neuroimaging in Non-human Primates

Single neurons and networks in the claustrum integrate input from widespread cortical sources

Temporal origin of mouse claustrum and development of its cortical projections

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