Enhancement the Biodegradation of Sodium Dodecyl Sulfate by Pseudomonas aeruginosa and Pseudomonas otitidis Isolated from Waste Water in Saudi Arabia

Metallo-β-lactamases (MBLs) encoding carbapenem resistance in wastewater are a well-known serious threat to human health. Twelve Pseudomonas otitidis isolates obtained from a municipal wastewater treatment plant (WWTP) in Hawaii were found to possess a subclass B3 MBL – POM ( P. otitidis βMBL), with a minimum inhibition concentration (MIC) range of 8–16 mg/L. The unrooted neighbor-joining phylogenetic tree showed that these blaPOM genes isolated in wastewater samples (n = 12) were distinctly different from other reference genes isolated from clinical, freshwater, animal, and soil samples except for isolates MR7, MR8, and MR11. MR7, MR8, and MR11 were found to have 4, 3, and 3 amino acid substitutions when compared to the type strain MC10330T and were closely clustered to the clinical reference genes. The meropenem hydrolysis experiment showed that isolates with multiple amino acid substitutions completely hydrolyzed 64 mg/L of meropenem in 7 h. The emergence of the opportunistic pathogen P. otitidis chromosomally encoding blaPOM in the treated municipal wastewater is an alarming call for the spread of this MBL in the environment. Further studies are required to understand the mechanism and regulation of this carbapenem-resistant β-lactamase in order to fill in the knowledge gap.

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Enhancement the Biodegradation of Sodium Dodecyl Sulfate by Pseudomonas aeruginosa and Pseudomonas otitidis Isolated from Waste Water in Saudi Arabia

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Biodegradation potential of sodium dodecyl sulphate (SDS) by mixed cells in domestic and non-domestic actual wastewaters: Experimental and kinetic studies

Biodegradation potential of sodium dodecyl sulphate (SDS) by mixed cells in domestic and non-domestic actual wastewaters: Experimental and kinetic studies

Diversity of blaPOM in carbapenem-resistant opportunistic pathogenic Pseudomonas otitidis in municipal wastewater

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