Enhancement the biocontrol efficacy of Sporidiobolus pararoseus Y16 against apple blue mold decay by glycine betaine and its mechanism

Abdelhai, Mandour H.; Awad, Faisal Nureldin; Yang, Qiya; Mahunu, Gustav Komla; Godana, Esa Abiso; Zhang, Hongyin

doi:10.1016/j.biocontrol.2019.104079

Cited by 29 publications

(14 citation statements)

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“…For example, Mahunu et al (2016) reported that Pichia caribbica with phytic acid can induce the PAL, POD, and CAT in apples which help the fruit to combat the blue mold disease. Other researchers also confirmed the use of yeast alone or in combination can significantly induce the major disease defense-related enzymes and phenolic compounds in post-harvest fruits and vegetables (Li and Tian, 2006;Al-Qurashi and Awad, 2015;Qin et al, 2015;Zhao et al, 2018;Abdelhai et al, 2019;Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 82%

Pichia anomala Induced With Chitosan Triggers Defense Response of Table Grapes Against Post-harvest Blue Mold Disease

et al. 2021

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To study the mechanism by which Pichia anomala induced with chitosan (1% w/v) controls blue mold disease in table grapes caused by Penicillium expansum, this study evaluated alterations in three yeast enzymatic activities. The changes in the five primary disease defense-related enzymes and two non-enzyme activities of table grapes were assayed. The results of the study showed that chitosan (1% w/v) significantly increased the yeast β-1,3-glucanase, catalase (CAT), and malondialdehyde (MDA) activities. Furthermore, P. anomala alone or induced with chitosan (1% w/v) significantly increased the table grapes enzymatic activities of Polyphenol oxidase (PPO), phenylalanine (PAL), peroxidase (POD), and catalase (CAT) compared to the control. The RT-qPCR results also confirmed that the genes of these major disease defense enzymes were up-regulated when the table grapes were treated with P. anomala. The highest results were recorded when the fruit was treated by yeast induced with chitosan (1% w/v). The phenolic compounds, in addition to their nutritional value, can also increase the antimicrobial properties of table grapes. The current experiment determined that the total phenol and flavonoid contents of table grapes showed the highest results for fruits treated by P. anomala induced with chitosan compared with the control. Generally, the increment of these fruit enzymatic and non-enzymatic activities shows improved table grape defense against the pathogenic fungus. The induction of the yeast with chitosan also increases its bio-control efficacy against the pathogen. This study will enable future detailed investigation in the yeast pathogen control mechanisms and the use of yeasts as bio-pesticides.

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Section: Introductionmentioning

confidence: 82%

Pichia anomala Induced With Chitosan Triggers Defense Response of Table Grapes Against Post-harvest Blue Mold Disease

et al. 2021

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“…After 6 days of incubation, disease incidence and lesion diameter were measured. Each treatment contained three replicates of 20 berries, and the experiment was carried out thrice 22 …”

Section: Methodsmentioning

confidence: 99%

“…Then, the culture was incubated in a rotary shaker for 20 h, at 180 rpm and 28 °C. After incubation, using a hemocytometer, the S. pararoseus Y16 concentration was determined and expressed as log10 cells mL −1 22 . There were three replications for each concentration of trehalose, and the whole experiment was conducted thrice.…”

Section: Methodsmentioning

confidence: 99%

“…Also, S. pararoseus had shown efficient biocontrol activity against a range of fungal pathogens, like Aspergillus niger of table grapes 21 Penicillium expansum on apples 9 . Recently, the biocontrol activity of S. pararoseus Y16 was enhanced against apple blue mold decay by culturing in glycine betaine supplemented media 22 . Glycine betaine‐induced S. pararoseus Y16 showed better biocontrol activity, induced fungal inhibitory activity without causing any adverse effects to fruit quality.…”

Section: Introductionmentioning

confidence: 99%

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Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Dhanasekaran

Yang

Godana

et al. 2021

Pest Management Science

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BACKGROUND In the process of biological control, the antagonistic yeasts contend with various stresses that negatively influence yeasts' biocontrol efficiency. In the current study, we investigated the effect of trehalose supplementation on the biocontrol efficiency and oxidative stress tolerance of Sporidiobolus pararoseus Y16. RESULTS S. pararoseus Y16, an antagonistic yeast cultured in trehalose supplemented medium, exhibited better biocontrol efficiency against Penicillium expansum and Aspergillus tubingensis in table grapes. Trehalose‐treated S. pararoseus Y16 cells showed good proliferation efficiency and oxidative stress tolerance than untreated cells. Increased β‐1,3‐glucanase, catalase, superoxide dismutase activity, and low protein carbonylation were observed in trehalose‐amended S. pararoseus Y16 upon H2O2 exposure. The RNA sequencing results indicated that trehalose significantly altered the transcriptome of S. pararoseus Y16. The GO, KEGG, and COG annotations revealed that the differentially regulated genes corresponded to the various biological process of the yeast. CONCLUSION Our findings suggested that trehalose use could enhance the biocontrol efficiency and oxidative stress tolerance of S. pararoseus Y16. Trehalose supplementation altered the transcriptome of S. pararoseus Y16, particularly the genes that correspond to amino acid metabolism, nucleotide metabolism, and protein modification. Thereby the oxidative stress tolerance and biological control efficiency of S. pararoseus Y16 was enhanced by trehalose. © 2021 Society of Chemical Industry.

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“…Sporidiobolus pararoseus Y16 and Baobab also substantially decreased the incidence of blue mold disease and lesion diameter (in vivo), and colony diameter (in vitro). Sporidiobolus pararoseus Y16 either in combination with Baobab or not, did not affect the quality parameters when stored with fruits (Abdelhai et al., 2019). The extract of Lentinula edodes , an edible mushroom, was also reported to enhance C. laurentii against P. expansum (Tolaini et al., 2010).…”

Section: Current Application Of Biotechnology In the Face Of The Foodmentioning

confidence: 99%

Recent trends in detecting, controlling, and detoxifying of patulin mycotoxin using biotechnology methods

Ngea

Yang

Castoria

et al. 2020

Comp Rev Food Sci Food Safe

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Patulin (PAT) is a mycotoxin that can contaminate many foods and especially fruits and fruit‐based products. Therefore, accurate and effective testing is necessary to enable producers to comply with regulations and promote food safety. Traditional approaches involving the use of chemical compounds or physical treatments in food have provided practical methods that have been used to date. However, growing concerns about environmental and health problems associated with these approaches call for new alternatives. In contrast, recent advances in biotechnology have revolutionized the understanding of living organisms and brought more effective biological tools. This review, therefore, focuses on the study of biotechnology approaches for the detection, control, and mitigation of PAT in food. Future aspects of biotechnology development to overcome the food safety problem posed by PAT were also examined. We find that biotechnology advances offer novel, more effective, and environmental friendly approaches for the control and elimination of PAT in food compared to traditional methods. Biosensors represent the future of PAT detection and use biological tools such as aptamer, enzyme, and antibody. PAT prevention strategies include microbial biocontrol, the use of antifungal biomolecules, and the use of microorganisms in combination with antifungal molecules. PAT detoxification aims at the breakdown and removal of PAT in food by using enzymes, microorganisms, and various adsorbent biopolymers. Finally, biotechnology advances will be dependent on the understanding of fundamental biology of living organisms regarding PAT synthesis and resistance mechanisms.

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Enhancement the biocontrol efficacy of Sporidiobolus pararoseus Y16 against apple blue mold decay by glycine betaine and its mechanism

Cited by 29 publications

References 29 publications

Pichia anomala Induced With Chitosan Triggers Defense Response of Table Grapes Against Post-harvest Blue Mold Disease

Pichia anomala Induced With Chitosan Triggers Defense Response of Table Grapes Against Post-harvest Blue Mold Disease

Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Recent trends in detecting, controlling, and detoxifying of patulin mycotoxin using biotechnology methods

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