Enhancement of β-Galactosidase Gene Expression in Rat Pheochromocytoma Cells by Exposure to Extremely Low Frequency Magnetic Fields

Ohtsu, Shuji; Miyakoshi, Junji; Tsukada, Toshihiko; Hiraoka, Masahiro; Abe, M.; Takebe, Hiraku

doi:10.1006/bbrc.1995.1942

Cited by 24 publications

(3 citation statements)

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“…Very similar findings using a 50 Hz 0.1 mT magnetic field and 2 Gy Gamma rays have been reported at the protein level (c-Jun, c-Fos) in epithelial cells [Lagroye and Poncy, 1998]. It has been reported that exposure to a pulsed magnetic field activated opioid gene expression in myocytes [Ventura et al, 2000] and enhanced b-galactosidase gene expression in rat pheochromocytoma cells [Ohtsu et al, 1995]. The present study demonstrates that GAP-43 is another gene that is responsive to a magnetic field.…”

Section: Discussionsupporting

confidence: 51%

Exposure to power frequency magnetic fields and X‐Rays induces GAP‐43 gene expression in human glioma MO54 cells

Ding

Nakahara

Miyakoshi

2002

Bioelectromagnetics

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We investigated the distribution and expression of growth associated protein-43 (GAP-43) in human glioma cells (MO54) after exposure to a magnetic field (60 Hz, 5 mT), with or without initial X-ionizing radiation (2 Gy), by using immunocytochemistry and the reverse transcription polymerase chain reaction (RT-PCR). GAP-43 was present in the cytoplasm, accumulating in the perinuclear area. An increase in GAP-43 expression was observed with a peak at 10 h at the mRNA level and at 12 h at the protein level, after exposure to the magnetic field. The increased level of GAP-43 protein returned to a normal level within 24 h of exposure to a 5 mT magnetic field. The kinetic pattern of GAP-43 expression induced by X-ionizing radiation was very similar to that induced by the magnetic field. These results suggest that the stimulation of GAP-43 expression could occur by a similar mechanism following exposure to X-rays or magnetic fields. We have provided the first evidence that exposure to a 5 mT magnetic field can induce GAP-43 gene expression in human glioma MO54 cells.

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Section: Discussionsupporting

confidence: 51%

Exposure to power frequency magnetic fields and X‐Rays induces GAP‐43 gene expression in human glioma MO54 cells

Ding

Nakahara

Miyakoshi

2002

Bioelectromagnetics

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“…For example, in experiments using rats, it was reported that exposure to a 0.1 mT magnetic field promoted the onset of mammary gland tumors and ornithine decarboxylase activation [19]. Moreover, it is reported that when a 200 to 400 mT magnetic field was exposed to cells originating from rat pheochromocytoma (PC12-VG), change was observed in intracellular signaling, such as an influx of ions via the cell membrane [20]. Meanwhile, Bassett et al [21] reported that a 0.2 mT magnetic field was effective for healing bone fractures in human and Oshibuchi [22] reported that a 10 mT magnetic field was effective for healing wounds in mice.…”

Section: Discussionmentioning

confidence: 99%

Effect of a Magnetic Field on Drosophila under Supercooled Conditions

et al. 2012

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Under subzero degree conditions, free water contained in biological cells tends to freeze and then most living things die due to low temperatures. We examined the effect of a variable magnetic field on Drosophila under supercooled conditions (a state in which freezing is not caused even below the freezing point). Under such supercooled conditions with the magnetic field at 0°C for 72 hours, −4°C for 24 hours and −8°C for 1 hour, the Drosophila all survived, while all conversely died under the supercooled conditions without the magnetic field. This result indicates a possibility that the magnetic field can reduce cell damage caused due to low temperatures in living things.

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“…Such a negative message diverged first of all from our own data regarding the MF-changes found in cell-cycle kinetics (Eremenko et al, 1997), membrane fluidity (Volpe et al, 1998), macrophage apoptosis (Fanelli et al, 1999) and macrophage defence (Volpe, 2003). The MF-independence of Hb expression, established herewith, diverged moreover from a number of other observations: the action of AC MFs was suggested for the production of ras p21 in CCRF-CEM (Phillips, 1993;Phillips et al, 1993) and β-galactosidase in rat pheochromocytoma (Ohtsu et al, 1995) cells; the Figure 7. CGC-dependent biosynthetic rate of total protein in FL cells maintained in the absence of MF in a MSR.…”

Section: Discussionmentioning

confidence: 50%

Gene Expression in a Space-Simulating Magnetically Shielded Environment

Volpe

Eremenko²

2005

Environmentalist

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A magnetic-field nullification achieved in a magnetically shielded room or a magnetic field of 70 µT generated in a solenoid with a sinusoidal wave of 50 Hz plus 45 µT DC of Earth, although able to change the cell proliferation and the physico-chemical properties of the cell membrane, did not seem to significantly influence gene expression in Friend erythroleukemia cells. In fact, when the culture growth cycle of these cells underwent magnetic-field deprivation or irradiation, the transcriptional and translational rates, in both undifferentiating and dimethylsulfoxide-differentiating cultures, turned out to be irrelevant. This result was supported by the magneticfield independent expression of the genes for α and β hemoglobin chains.

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Enhancement of β-Galactosidase Gene Expression in Rat Pheochromocytoma Cells by Exposure to Extremely Low Frequency Magnetic Fields

Cited by 24 publications

References 0 publications

Exposure to power frequency magnetic fields and X‐Rays induces GAP‐43 gene expression in human glioma MO54 cells

Exposure to power frequency magnetic fields and X‐Rays induces GAP‐43 gene expression in human glioma MO54 cells

Effect of a Magnetic Field on Drosophila under Supercooled Conditions

Gene Expression in a Space-Simulating Magnetically Shielded Environment

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