Enhancement of X-Ray-Induced Breaks in DNA Bound to Molecules Containing Platinum: A Possible Application to Hadrontherapy

Kobayashi, Kazuo; Fröhlich, H.; Usami, Noriko; Takakura, Kaoru; Sech, C. Le

doi:10.1667/0033-7587(2002)157[0032:eoxrib]2.0.co;2

Cited by 57 publications

(37 citation statements)

References 17 publications

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“…Subsequent work explored analogous cleavage reactions for DNA in solution [61] and showed that, as with the dry film studies, platinated DNA was cleaved approximately twice as much as unplatinated DNA. However, the effect of DMSO as a solution radical scavenger and studies of variable wavelength excitation led researchers to conclude that hydroxyl radical formation is the major cleavage mechanism.…”

Section: Nucleic Acid Cleavagementioning

confidence: 91%

Platinum complexes of terpyridine: Interaction and reactivity with biomolecules

Cummings

2009

Coordination Chemistry Reviews

122

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Section: Nucleic Acid Cleavagementioning

confidence: 91%

Platinum complexes of terpyridine: Interaction and reactivity with biomolecules

Cummings

2009

Coordination Chemistry Reviews

122

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“…[2][3][4] In addition, NPs composed of high-Z elements such as noble metals or lanthanides amplify the effect of radiation treatment. 5 This phenomenon, which was first evidenced for platinum complexes combined with highenergy photons 6,7 and fast ions, 8 was later demonstrated for gold NPs (AuNPs) 9 and platinum NPs (PtNPs) 10 in combination with photons or fast ions. Subsequent simulation studies have led to first insights into this amplification effect.…”

Section: Introductionmentioning

confidence: 94%

Improving proton therapy by metal-containing nanoparticles: nanoscale insights

Schlathölter¹,

Eustache²,

Porcel³

et al. 2016

IJN

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The use of nanoparticles to enhance the effect of radiation-based cancer treatments is a growing field of study and recently, even nanoparticle-induced improvement of proton therapy performance has been investigated. Aiming at a clinical implementation of this approach, it is essential to characterize the mechanisms underlying the synergistic effects of nanoparticles combined with proton irradiation. In this study, we investigated the effect of platinum- and gadolinium-based nanoparticles on the nanoscale damage induced by a proton beam of therapeutically relevant energy (150 MeV) using plasmid DNA molecular probe. Two conditions of irradiation (0.44 and 3.6 keV/μm) were considered to mimic the beam properties at the entrance and at the end of the proton track. We demonstrate that the two metal-containing nanoparticles amplify, in particular, the induction of nanosize damages (>2 nm) which are most lethal for cells. More importantly, this effect is even more pronounced at the end of the proton track. This work gives a new insight into the underlying mechanisms on the nanoscale and indicates that the addition of metal-based nanoparticles is a promising strategy not only to increase the cell killing action of fast protons, but also to improve tumor targeting.

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“…For detailed analysis of x-ray absorption by heavy atoms, absorption of a mono-energetic photon should be examined using a tunable energy source such as synchrotron facilities. Use of a mono-energetic microbeam in radiobiology is already in progress elsewhere [18].…”

Section: Resultsmentioning

confidence: 99%

Analysis of Potential Radiosensitizing Materials for X-Ray-Induced Photodynamic Therapy

Takahashi

Misawa

2007

Nanobiotechnol

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For a development of deep tumor treatment in photodynamic therapy, a feasibility of novel radiosensitizers induced by x-ray was investigated. The sensitizers are designed to generate reactive oxygen species (ROS) inside or outside the cell, possibly leading to damage exclusively on tumor cells and reservation of normal cells along the x-ray path. Taking note of the similarity in energy transfer mechanism in photocatalysts, scintillators, and particulate semiconductors, we chose TiO 2 , ZnS:Ag, CeF 3 , and quantum dots (CdTe and CdSe) in particulate form, which contain heavy atoms for efficient absorption of x-rays. A parameter study for x-ray operating conditions showed that in a typical scenario, photons with 20 to 170 keV energy are attenuated by 90% through the region of particle dispersed aqueous solution at varying concentration between 0.01 and 100 wt%. The amount of ROS generation under the exposure of polychromatic x-ray was measured using dihydroethidium reagent which detects an integrated amount of several species. Proportional increase in ROS generation to x-ray dose was observed for varying concentrations of TiO 2 , ZnS:Ag, CeF 3 , and CdSe quantum dot dispersions. Then, HeLa cells were mixed with aqueous solutions dispersed with sensitizing materials at a concentration of 3.0 mg/ml and were exposed to x-ray. Their survival fraction obtained by a cell proliferation reagent WST-1 immediately after the irradiation showed insignificant effects of sensitizing materials except at large doses. To enhance the sensitization effect, bio-conjugated CdSe quantum dots were internalized in the cytoplasm up to a concentration of 1.0 ng/ml. The cells were irradiated by x-ray up to 5 Gy, and their survival fraction was measured by the colony forming ability 9 days after irradiation. Survival fraction of the cells treated with quantum dots were less than those without quantum dots for all doses, suggesting that the colony forming ability is impaired by the internalized quantum dots.

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Enhancement of X-Ray-Induced Breaks in DNA Bound to Molecules Containing Platinum: A Possible Application to Hadrontherapy

Cited by 57 publications

References 17 publications

Platinum complexes of terpyridine: Interaction and reactivity with biomolecules

Platinum complexes of terpyridine: Interaction and reactivity with biomolecules

Improving proton therapy by metal-containing nanoparticles: nanoscale insights

Analysis of Potential Radiosensitizing Materials for X-Ray-Induced Photodynamic Therapy

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