Enhancement of vitamin E production in sunflower cell cultures

Caretto, Sofia; Speth, Elena Bray; Fachechi, Christian; Gala, Rosa; Zacheo, G.; Giovinazzo, Giovanna

doi:10.1007/s00299-004-0799-6

Cited by 40 publications

(36 citation statements)

References 19 publications

(31 reference statements)

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“…Callus and cell suspension cultures were induced from plantlets where a-tocopherol was always more than 91% of the total tocopherols. It is interesting to note that such a percentage was maintained in cell cultures and the average a-tocopherol content was similar to that measured in the starting tissue (Table 2) [21,22]. Also callus cultures induced from safflower (Carthamus tinctorius) flower buds mainly accumulated a-tocopherol [23].…”

Section: Establishment Of In Vitro Cell Culturesmentioning

confidence: 71%

“…The most efficient medium was found to be MS basal medium containing naphthaleneacetic acid and 6-benzylaminopurine with the addition of casaminoacids and myo-inositol. Furthermore, a 78% increase of a-tocopherol in cell suspensions in the stationary phase was obtained by selecting cells for longer subculture intervals, probably due to the response to a stress condition (Table 2) [22]. The ability of casaminoacids and myo-inositol to enhance tocopherol production had already been observed in safflower cell cultures [23].…”

Section: Establishment Of In Vitro Cell Culturesmentioning

confidence: 94%

“…When the biosynthetic precursor of the polar head, homogentisic acid, was administered to sunflower suspension cultures, a clear stimulatory effect (30% increase) on the production of a-tocopherol was observed, suggesting that this compound can be useful for improving the yield of sunflower cell cultures. On the other hand, the precursor of the hydrophobic tail, phytol, could not increase a-tocopherol production on its own [22].…”

Section: Enhancement Of Tocopherol Productionmentioning

confidence: 99%

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Tocopherol production in plant cell cultures

Caretto

Nisi

Paradiso

et al. 2010

Molecular Nutrition Food Res

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Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, essential dietary components for mammals and exclusively synthesized by photosynthetic organisms. Of the four forms (alpha, beta, gamma and delta), alpha-tocopherol is the major vitamin E form present in green plant tissues, and has the highest vitamin E activity. Synthetic alpha-tocopherol, being a racemic mixture of eight different stereoisomers, always results less effective than the natural form (R,R,R) alpha-tocopherol. This raises interest in obtaining this molecule from natural sources, such as plant cell cultures. Plant cell and tissue cultures are able to produce and accumulate valuable metabolites that can be used as food additives, nutraceuticals and pharmaceuticals. Sunflower cell cultures, growing under heterotrophic conditions, were exploited to establish a suitable in vitro production system of natural alpha-tocopherol. Optimization of culture conditions, precursor feeding and elicitor application were used to improve the tocopherol yields of these cultures. Furthermore, these cell cultures were useful to investigate the relationship between alpha-tocopherol biosynthesis and photomixotrophic culture conditions, revealing the possibility to enhance tocopherol production by favouring sunflower cell photosynthetic properties. The modulation of alpha-tocopherol levels in plant cell cultures can provide useful hints for a regulatory impact on tocopherol metabolism.

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Section: Establishment Of In Vitro Cell Culturesmentioning

confidence: 71%

Section: Establishment Of In Vitro Cell Culturesmentioning

confidence: 94%

Section: Enhancement Of Tocopherol Productionmentioning

confidence: 99%

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Tocopherol production in plant cell cultures

Caretto

Nisi

Paradiso

et al. 2010

Molecular Nutrition Food Res

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“…These data indicate that the availability of both tocopherol precursors restricts vitamin E biosynthesis, with phytol being the most limiting. In contrast, in sunflower suspension cells, HGA feeding stimulated tocopherol accumulation ($30%), while phytol supplementation had no effect [40]. Finally, in soybean suspension cultures, tocopherol content increased twofold in response to either HGA or phytol feeding, and fivefold when both precursors were added simultaneously [15].…”

Section: Tocochromanolmentioning

confidence: 95%

“…Activity (IU/mg) Activity (%) In order to identify the biosynthetic steps limiting tocochromanol accumulation in plants, tocopherol production was investigated in cell cultures fed with metabolic intermediates [15,39,40]. While these studies concluded that both HGA and phytol had the greatest effects on vitamin E biosynthesis, their conclusions differ on which compound is the most limiting.…”

Section: Tocochromanolmentioning

confidence: 99%

Current strategies for vitamin E biofortification of crops

Mène-Saffrané

Pellaud

2017

Current Opinion in Biotechnology

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Vitamin E refers to four tocopherols and four tocotrienols that are exclusively synthesized by photosynthetic organisms. While a-tocopherol is the most potent vitamin E compound, it is not the main form consumed since the composition of most major crops is dominated by g-tocopherol. Nutritional studies show that populations of developed countries do not consume enough vitamin E and that a large proportion of individuals exhibit plasma a-tocopherol deficiency. Following the identification of vitamin E biosynthetic genes, several strategies including metabolic engineering, classic breeding and mutation breeding, have been undertaken to improve the vitamin E content of crops. In addition to providing crops in which vitamin E content is enhanced, these studies are revealing the bottlenecks limiting its biosynthesis.

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Cyclodextrins increase phytosterol and tocopherol levels in suspension cultured cells obtained from mung beans and safflower

Almagro

Tudela

Sabater-Jara

et al. 2017

Biotechnology Progress

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In this work, suspension-cultured cells of mung beans and safflower were used in order to analyze the effect of methyl jasmonate and/or cyclodextrins, on bioactive compound production such as phytosterols and tocopherols. The results indicated that mung bean suspension-cultured cells produced higher amount of total phytosterols and tocopherols. In particular, mung bean suspension-cultured cells produced almost 220-fold higher levels of tocopherols than safflower suspension-cultured cells in the best conditions. However, while cyclodextrins were able to enhance extracellular production of phytosterols, in the case of tocopherols, they only increased their intracellular accumulation. Our results showed that mung bean cells could be used as a highly efficient system for the production of phytosterols and tocopherols which have a wide range of biological activities. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1662-1665, 2017.

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Enhancement of vitamin E production in sunflower cell cultures

Cited by 40 publications

References 19 publications

Tocopherol production in plant cell cultures

Tocopherol production in plant cell cultures

Current strategies for vitamin E biofortification of crops

Cyclodextrins increase phytosterol and tocopherol levels in suspension cultured cells obtained from mung beans and safflower

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