Enhancement of the transfection efficiency of DNA into Crocus sativus L. cells via PEI nanoparticles

Firoozi, Behnam; Zare, Nasser; Sofalian, Omid; Sheikhzade-Mosadegh, Parisa

doi:10.1016/s2095-3119(18)61985-9

Cited by 10 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PEI has many potential applications due to its polycationic character and water solubility [33]. In the biomedical field, it can be used as an antiviral agent in DNA transfection because it is able to interact with negatively charged DNA and RNA [33][34][35][36]. It is used for separation and purification of biomacromolecules, enzyme immobilization, biosensor construction, and drug delivery [33,[37][38][39][40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles of Quaternary Ammonium Polyethylenimine Derivatives for Application in Dental Materials

Chrószcz-Porębska

Barszczewska-Rybarek

2020

Polymers

View full text Add to dashboard Cite

Various quaternary ammonium polyethylenimine (QA-PEI) derivatives have been synthesized in order to obtain nanoparticles. Due to their antibacterial activity and non-toxicity towards mammalian cells, the QA-PEI nanoparticles have been tested extensively regarding potential applications as biocidal additives in various dental composite materials. Their impact has been examined mostly for dimethacrylate-based restorative materials; however, dental cements, root canal pastes, and orthodontic adhesives have also been tested. Results of those studies showed that the addition of small quantities of QA-PEI nanoparticles, from 0.5 to 2 wt.%, led to efficient and long-lasting antibacterial effects. However, it was also discovered that the intensity of the biocidal activity strongly depended on several chemical factors, including the degree of crosslinking, length of alkyl telomeric chains, degree of N-alkylation, degree of N-methylation, counterion type, and pH. Importantly, the presence of QA-PEI nanoparticles in the studied dental composites did not negatively impact the degree of conversion in the composite matrix, nor its mechanical properties. In this review, we summarized these features and functions in order to present QA-PEI nanoparticles as modern and promising additives for dental materials that can impart unique antibacterial characteristics without deteriorating the products’ structures or mechanical properties.

show abstract

Section: Introductionmentioning

confidence: 99%

Nanoparticles of Quaternary Ammonium Polyethylenimine Derivatives for Application in Dental Materials

Chrószcz-Porębska

Barszczewska-Rybarek

2020

Polymers

View full text Add to dashboard Cite

show abstract

“…Its main application is as a nonviral vector for DNA transfer in gene therapy, including regenerative medicine [78], AIDS, cancer, and hereditary disorder treatments [79]. It has also been used for transfection and editing of genomes in plants [84,85], as well as for the gene silencing systems mentioned above aimed at disease and pest control [22,47,52,86,87].…”

Section: Polyplex Nanoparticlesmentioning

confidence: 99%

RNAi Crop Protection Advances

Hernández-Soto

Chacón-Cerdas

2021

IJMS

View full text Add to dashboard Cite

RNAi technology is a versatile, effective, safe, and eco-friendly alternative for crop protection. There is plenty of evidence of its use through host-induced gene silencing (HIGS) and emerging evidence that spray-induced gene silencing (SIGS) techniques can work as well to control viruses, bacteria, fungi, insects, and nematodes. For SIGS, its most significant challenge is achieving stability and avoiding premature degradation of RNAi in the environment or during its absorption by the target organism. One alternative is encapsulation in liposomes, virus-like particles, polyplex nanoparticles, and bioclay, which can be obtained through the recombinant production of RNAi in vectors, transgenesis, and micro/nanoencapsulation. The materials must be safe, biodegradable, and stable in multiple chemical environments, favoring the controlled release of RNAi. Most of the current research on encapsulated RNAi focuses primarily on oral delivery to control insects by silencing essential genes. The regulation of RNAi technology focuses on risk assessment using different approaches; however, this technology has positive economic, environmental, and human health implications for its use in agriculture. The emergence of alternatives combining RNAi gene silencing with the induction of resistance in crops by elicitation and metabolic control is expected, as well as multiple silencing and biotechnological optimization of its large-scale production.

show abstract

“…Its main application is as a non-viral vector for DNA transfer in gene therapy including regenerative medicine 70 , AIDS, cancer, and hereditary disorders treatment 71 . It has also increased its use for transfection and editing of genomes in plants 76,77 , as well as for gene silencing systems mentioned above aimed to disease and pest control 23,38,44,78,79 .…”

Section: Polyplex Nanoparticlesmentioning

confidence: 99%

RNAi Crop Protection Advances

Hernández-Soto¹,

Chacón-Cerdas²

2021

Preprint

View full text Add to dashboard Cite

RNAi technology is a versatile, effective, safe, and eco-friendly alternative for crop protection. There is plenty of evidence of its use through Host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS) techniques to control viruses, bacteria, fungi, insects, and nematodes. As for SIGS, its most significant challenge is achieving stability and avoiding premature degradation of RNAi in the environment or during its absorption in the target organism. One alternative is the encapsulation in liposomes, virus-like particles, polyplex nanoparticles, and bio-clay, which can be obtained through the recombinant production of RNAi in vectors, transgenesis, and micro/nanoencapsulation. The materials must be safe, biodegradable, and stable in multiple chemical environments favoring the controlled release of RNAi. Most of the current research of encapsulated RNAi focuses primarily on oral delivery to control insects by silencing essential genes. The regulation of RNAi technology focuses on risk assessment from different approaches; however, this technology has positive characteristics for its use in agriculture from the economic, environmental, and human health implications. The emergence of alternatives combining RNAi gene silencing with the induction of resistance in crops by elicitation and metabolic control is expected, as well as multiple silencing and biotechnological optimization of its large-scale production.

show abstract

Enhancement of the transfection efficiency of DNA into Crocus sativus L. cells via PEI nanoparticles

Cited by 10 publications

References 31 publications

Nanoparticles of Quaternary Ammonium Polyethylenimine Derivatives for Application in Dental Materials

Nanoparticles of Quaternary Ammonium Polyethylenimine Derivatives for Application in Dental Materials

RNAi Crop Protection Advances

RNAi Crop Protection Advances

Contact Info

Product

Resources

About