Enhancement of the immunogenicity of an infectious laryngotracheitis virus DNA vaccine by a bicistronic plasmid encoding glycoprotein B and interleukin-18

Chen, Hongying; Zhao, Li; Wei, Zhanyong; Bao-an, Cui; Wang, Zhenya; Li, Xinsheng; Xia, Pingan; Liu, Jinpeng

doi:10.1016/j.antiviral.2010.05.009

Cited by 24 publications

(11 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These findings were consistent with previous reports that IL-18 yielded stronger CD4+ T cell responses in immunized mice [13] and chickens [34] thus enabling DNA vaccines to achieve enhanced immune responses. On the other hand, the reports also showed that the co-administration of the IL-18 plasmid as an encoded adjuvant yielded stronger CD8+ T cells [35]. However, both trials in the present study revealed that pDis/IL-18 was not significant (P > 0.05) in inducing CD8+ T cells in the inoculation group.…”

Section: Discussioncontrasting

confidence: 84%

Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

et al. 2012

View full text Add to dashboard Cite

BackgroundDNA vaccines offer several advantages over conventional vaccines in the development of effective vaccines against avian influenza virus (AIV). However, one of the limitations of the DNA vaccine in poultry is that it induces poor immune responses. In this study, chicken interleukin (IL) -15 and IL-18 were used as genetic adjuvants to improve the immune responses induced from the H5 DNA vaccination in chickens. The immunogenicity of the recombinant plasmid DNA was analyzed based on the antibody production, T cell responses and cytokine production, following inoculation in 1-day-old (Trial 1) and 14-day-old (Trial 2) specific-pathogen-free chickens. Hence, the purpose of the present study was to explore the role of chicken IL-15 and IL-18 as adjuvants following the vaccination of chickens with the H5 DNA vaccine.ResultsThe overall HI antibody titer in chickens immunized with pDis/H5 + pDis/IL-15 was higher compared to chickens immunized with pDis/H5 (p < 0.05). The findings revealed that the inoculation of the 14-day-old chickens exhibited a shorter time to achieve the highest HI titer in comparison to the inoculation of the 1-day-old chickens. The cellular immunity was assessed by the flow cytometry analysis to enumerate CD4+ and CD8 + T cells in the peripheral blood. The chickens inoculated with pDis/H5 + pDis/IL-15 demonstrated the highest increase in CD4+ T cells population relative to the control chickens. However, this study revealed that pDis/H5 + pDis/IL-15 was not significant (P > 0.05) in inducing CD8+ T cells. Meanwhile, with the exception of Trial 1, the flow cytometry results for Trial 2 demonstrated that the pDis/H5 + pDis/IL-18 inoculated group was able to trigger a higher increase in CD4+ T cells than the pDis/H5 group (P < 0.05). On the other hand, the pDis/H5 + pDis/IL-18 group was not significant (P > 0.05) in modulating CD8+ T cells population in both trials. The pDis/H5 + pDis/IL-15 inoculated group showed the highest IL-15 gene expression in both trials compared to other inoculated groups (P < 0.05). Similar results were obtained for the IL-18 expression where the pDis/H5 + pDis/IL-18 groups in both trials (Table 8) were significantly higher compared to the control group (P < 0.05). However, the expressions of other cytokines remained low or undetected by GeXP assay.ConclusionsThis study shows the diverse immunogenicity of pDis/H5 co-administered with chicken IL-15 and IL-18,with pDis/H5 + pDis/IL-15 being a better vaccine candidate compared to other groups.

show abstract

Section: Discussioncontrasting

confidence: 84%

Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

et al. 2012

View full text Add to dashboard Cite

show abstract

“…A AILTV vector with the HPAI H5 gene inserted in the deleted region of the UL50 gene protected chickens against challenges with homologous and heterologous H5N1 and H5N2 viruses, respectively (Pavlova et al, 2009). Another vaccine ILTV was developed using the gB gene combined with chicken IL-18 as a bicistronic vector and induced better protection of chickens challenged with ILTV that those containing only the gB gene as monocistronic vector (Chen et al, 2010).…”

Section: Epidemiology Of Avian Infectious Laryngotracheitis With Specmentioning

confidence: 99%

Epidemiology of Avian Infectious Laryngotracheitis with Special Focus to South America: an update

Parra

Nuñez

Ferreira

2016

Rev. Bras. Cienc. Avic.

View full text Add to dashboard Cite

“…For the Marek disease virus, a DNA vaccine composed of a bacterial artificial chromosome encoding the herpesvirus genome permitted to 55% of the chickens to survive a highly virulent challenge (no survivors in the control group) [50]. Likewise, a DNA vaccine against infectious laryngotracheitis virus encoding the glycoprotein B resulted in the decrease of the mortality from 65-75% to 0% [12]. Finally, a DNA vaccine encoding the major component of the virion envelop gp90 of the reticuloendothelis virus induced the production of neutralizing antibodies and lymphoproliferation resulting in the control of the viremia after challenge [51].…”

Section: Different Poultry Dna Vaccination Modelsmentioning

confidence: 99%

“…Some of these conventional vaccines are not fully efficient due, for example, to the presence of maternal antibodies in young birds [5,6] or to the existence of multiple pathogenic strains and/or the emergence of new strains [7][8][9][10]. Furthermore, there is a risk with some conventional vaccines of reversion to virulence in certain cases [3,11] or of adverse reactions, especially in young birds [2,12,13]. Several vaccines offer no way of differentiating infected from vaccinated animals (DIVA) [13].…”

Section: Introductionmentioning

confidence: 99%

DNA vaccination of poultry: The current status in 2015

Meunier

Chemaly

Dory

2016

Vaccine

View full text Add to dashboard Cite

DNA vaccination is a promising alternative strategy for developing new human and animal vaccines. The massive efforts made these past 25 years to increase the immunizing potential of this kind of vaccine are still ongoing. A relatively small number of studies concerning poultry have been published. Even though there is a need for new poultry vaccines, five parameters must nevertheless be taken into account for their development: the vaccine has to be very effective, safe, inexpensive, suitable for mass vaccination and able to induce immune responses in the presence of maternal antibodies (when appropriate). DNA vaccination should meet these requirements. This review describes studies in this field performed exclusively on birds (chickens, ducks and turkeys). No evaluations of avian DNA vaccine efficacy performed on mice as preliminary tests have been taken into consideration. The review first describes the state of the art for DNA vaccination in poultry: pathogens targeted, plasmids used and different routes of vaccine administration. Second, it presents strategies designed to improve DNA vaccine efficacy: influence of the route of administration, plasmid dose and age of birds on their first inoculation; increasing plasmid uptake by host cells; addition of immunomodulators; optimization of plasmid backbones and codon usage; association of vaccine antigens and finally, heterologous prime-boost regimens. The final part will indicate additional properties of DNA vaccines in poultry: fate of the plasmids upon inoculation, immunological considerations and the use of DNA vaccines for purposes other than preventing infectious diseases.

show abstract

Enhancement of the immunogenicity of an infectious laryngotracheitis virus DNA vaccine by a bicistronic plasmid encoding glycoprotein B and interleukin-18

Cited by 24 publications

References 37 publications

Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

Epidemiology of Avian Infectious Laryngotracheitis with Special Focus to South America: an update

DNA vaccination of poultry: The current status in 2015

Contact Info

Product

Resources

About