Enhancement of testosterone secretion by normal adult human Leydig cells by co‐culture with enriched preparations of normal adult human Sertoli cells

Lejeune, Hervé; Skalli, Mourad; Sanchez, Pierre; Avallet, O.; Saez, J.M.

doi:10.1111/j.1365-2605.1993.tb01149.x

Cited by 32 publications

(24 citation statements)

References 23 publications

(44 reference statements)

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“…Here we choose sertoli cells as the starting cells, which could also be isolated from adult human and nonhuman primate and cultured at high purity in vitro [24][25][26][27][28], and show that mesoderm-derived terminally differentiated sertoli cells can be directly converted to a multipotent state of ectodermal neural lineage by a combination of nine transcription factors. The global gene expression of iNSCs has been broadly reprogrammed from the original sertoli cell patterns to a profile similar but not identical to that of normal neural stem cells.…”

Section: Discussionmentioning

confidence: 99%

Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors

et al. 2011

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Multipotent neural stem/progenitor cells hold great promise for cell therapy. The reprogramming of fibroblasts to induced pluripotent stem cells as well as mature neurons suggests a possibility to convert a terminally differentiated somatic cell into a multipotent state without first establishing pluripotency. Here, we demonstrate that sertoli cells derived from mesoderm can be directly converted into a multipotent state that possesses neural stem/progenitor cell properties. The induced neural stem/progenitor cells (iNSCs) express multiple NSC-specific markers, exhibit a global gene-expression profile similar to normal NSCs, and are capable of self-renewal and differentiating into glia and electrophysiologically functional neurons. iNSC-derived neurons stain positive for tyrosine hydroxylase (TH), γ-aminobutyric acid, and choline acetyltransferase. In addition, iNSCs can survive and generate synapses following transplantation into the dentate gyrus. Generation of iNSCs may have important implications for disease modeling and regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors

et al. 2011

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“…Rat and mouse Sertoli and peritubular cells were prepared from 20-day-old and 15-dayold animals, respectively, as described by Toebosch et al [26]. Human Sertoli cells were prepared as described by Lejeune et al [27]. These cell types were then cultured in appropriate media at 32ЊC in a humidified atmosphere (5% CO 2 , 95% air).…”

Section: Cells and Culturesmentioning

confidence: 99%

“…The cells were then used for RNA and protein extraction and uptake studies. Human Leydig cells were prepared and cultured as described elsewhere [27] before being used for RNA extraction.…”

Section: Cells and Culturesmentioning

confidence: 99%

Multidrug Resistance Genes and P-Glycoprotein in the Testis of the Rat, Mouse, Guinea Pig, and Human1

Melaine

Liénard

Dorval

et al. 2002

Biology of Reproduction

113

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Study of the multidrug resistance phenomenon in tumor cell lines has led to the discovery of the product of the multidrug resistance (MDR) type 1 genes, the plasma membrane P-glycoprotein (P-gp) that functions as an energy-dependent pump for the efflux of diverse anticancer drugs. P-gp was also recently identified in normal epithelial cells with secretory/excretory functions and in the endothelial cells of the capillary blood vessels in the brain and the testis. These endothelial cells are key elements of the blood-brain and blood-testis barriers, respectively. The aim of this study, in the rat, mouse, guinea pig, and human, was to determine whether testicular cells other than the capillary endothelial cells could express MDR type I genes. Immunohistochemistry on testicular sections revealed that P-gp is present in interstitial cells in the mouse, rat, and human testes, in early and late spermatids in guinea pig testis, and in late spermatids in the rat, mouse, and human. Reverse transcription-polymerase chain reaction analysis on isolated mouse, rat, and human cells showed that all somatic testicular cells (Leydig cells, macrophages, peritubular cells, and Sertoli cells) and the cytoplasmic lobes from rat late spermatids expressed MDR type I mRNAs, whereas spermatogonia, pachytene spermatocytes, and early spermatids did not. An ontogenesis study in the mouse reveals that type I MDR gene expression begins at 13.5 days postcoitum at the time when the seminiferous cords and the blood vessels appear and are maintained thereafter. Finally, two functional tests on isolated rat cells, the doxorubicin and rhodamine uptake assays, demonstrated that rat testicular macrophages, Leydig cells, peritubular cells, and Sertoli cells displayed a multidrug-resistance activity, whereas spermatogonia, pachytene spermatocytes, and early spermatids did not. Western blot experiments have revealed that a P-gp of 175 kDa is present in the human testis as well as in the rat Leydig cells, testicular macrophages, peritubular cells, and Sertoli cells, but is absent in spermatogonia, spermatocytes, and early spermatids. We conclude that P-gp is involved in the self-protection of the somatic cells and is most probably one of the molecules that confers its functionality to the blood-testis barrier. The absence of expression of MDR type I genes in mitotic and meiotic germ cells probably explains their particular vulnerability to various anticancer drugs. In contrast, expression of the P-gp in the haploid cells most likely reflects the ability of spermatozoa to assume their own antidrug defense.

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“…This mechanism might be operative in vivo, in normal children. There are in vitro evidences (22) (26).…”

Section: Discussionmentioning

confidence: 99%

Basal Testosterone Secretion and Response to Human Luteinizing, Follicle-Stimulating, and Growth Hormones in Culture of Cells Isolated from Testes of Infants and Children

et al. 1995

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Little is known on the hormonal regulation of the early postnatal phase of Leydig cell activation in the human. Testosterone secretion by prepubertal testicular cells in culture was studied in two different age groups, 0-7-mo-old (group 1) and 16-36-mo-old (group 2) boys. A mixed cell preparation was isolated from testes collected at necropsy and maintained in culture for 6 d. Cells were cultured in serum-free medium in basal conditions and under the stimulation of human (h)LH, hFSH, or recombinant hGH, and the secretion of testosterone was determined on d 6 by RIA. In basal conditions, cells of group 1 secreted more testosterone (median 5.83 pmol/106 cellsd, n =

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Enhancement of testosterone secretion by normal adult human Leydig cells by co‐culture with enriched preparations of normal adult human Sertoli cells

Cited by 32 publications

References 23 publications

Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors

Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors

Multidrug Resistance Genes and P-Glycoprotein in the Testis of the Rat, Mouse, Guinea Pig, and Human1

Basal Testosterone Secretion and Response to Human Luteinizing, Follicle-Stimulating, and Growth Hormones in Culture of Cells Isolated from Testes of Infants and Children

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