Enhancement of Taxane Production by the Combination of <i>in situ</i> Extraction with Lauryl Alcohol and Elicitation by Methyl Jasmonate in a Suspension Callus Culture

Abstract

We report here on taxane production by a combination of in situ extraction with lauryl alcohol (LA) and elicitation by methyl jasmonate (MJ) in a suspension callus of Taxus cuspidata. LA is an effective organic solvent for the in situ extraction of paclitaxel which gives a decrease in the paclitaxel concentration in the medium thus reducing its feed-back inhibition against callus growth. Improved callus growth and increased production amounts of the taxanes were observed in the two phase systems including MJ, compared with the control culture where LA and MJ were not added. In particular significant amounts of 10-deacetyl baccatin III and baccatin III, which are precursors of paclitaxel, were produced, indicating that the rate-limiting steps in the production of paclitaxel might be the enzymatic activities of converting baccatin III to paclitaxel and/or the genes expression of the related enzymes.

5‐Aminolevulinic acid promotes callus growth and paclitaxel production in light‐grown Taxus cuspidata suspension cultures

“…Both these systems increased paclitaxel productivity [20][21][22][23][24]. Enhancements of callus growth and paclitaxel productivity in a lauryl alcohol (LA) medium two-phase culture system were achieved in previous studies [11,19,25,26]. In these culture systems, addition of MJ enhanced paclitaxel production but resulted in little callus growth [6].…”

“…For effective paclitaxel production, the cultures must be able to sustain growth while producing large amounts of paclitaxel simultaneously. To reduce inhibition caused by paclitaxel accumulation, two-phase culture systems using waterimmiscible organic solvents or solid absorbents have been used to recover paclitaxel from the culture medium in situ [17,[19][20][21][22][23][24][25][26][27]. Both these systems increased paclitaxel productivity [20][21][22][23][24].…”

Transcriptomics and Physiological Analyses Reveal Changes in Paclitaxel Production and Physiological Properties in Taxus cuspidata Suspension Cells in Response to Elicitors

“…These phenomena were such because three elicitors generate different defense response signaling, but they are cross-linked in the signaling pathway and showed synergistic effects in increasing paclitaxel production. Other scholars have treated sequoia healing tissues, suspension cells, or hazelnut cells with CD + methyl jasmonate [57], SA + glucose [30], lauryl alcohol + methyl jasmonate [15], and ultrasound + SA [58], respectively; on this, they found that the paclitaxel yields were significantly higher than those obtained following single treatments. In particular, the paclitaxel yield of the Taxus Chinese suspension cells with SA and fungal elicitors was 1.5 times, 2.3 times, and 7.5 times higher than that of the fungal elicitor treatment group, SA treatment group, and the Control group, respectively [40].…”

“…Paclitaxel is a hydrophobic substance with cytotoxic properties [14]. Large accumulations in suspension cells inhibit cell growth and affect paclitaxel production [15].…”

“…Paclitaxel is a hydrophobic substance with cytotoxic properties [14]. Large accumulations in suspension cells inhibit cell growth and affect paclitaxel production [15]. Therefore, there is an urgent need to induce paclitaxel exocytosis without decreasing cellular activity and paclitaxel synthesis capacity.…”