Enhancement of sucrose metabolism in Clostridium saccharoperbutylacetonicum N1-4 through metabolic engineering for improved acetone–butanol–ethanol (ABE) fermentation

A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains

“…Renewable sugar, cellulose, and starch biomass are important substrates in industrial ABE fermentation. Sucrose is a cheap and ideal feedstock for ABE fermentation [15]. In C. beijerinckii 8052 and C. acetobutylicum DSM 792, 14 C-sucrose isotope labelling was employed to demonstrate that sucrose transport occurs via a PEP-PTS mechanism [23,25,26,27].…”

“…Finding new metabolic pathways to increase sucrose consumption by Clostridium is one way to use this inexpensive and desirable substrate for ABE fermentation. Recently, Zhang et al enhanced endogenous and introduced heterologous sucrose utilization pathways into C. saccharoperbutylacetonicum [15]. In their work, overexpression of the endogenous sucrose pathway increased sucrose consumption and ABE production.…”

“…Butanol is a renewable biofuel that can be produced by fermentation in Clostridium [21,22], but the ability of Clostridium to use sucrose is low [23,24]. Recombinant engineering of Clostridium to achieve high levels of sucrose transport would aid sucrose consumption in Clostridium to produce butanol [15]. In this work, we show that heterologously expressed StSUT1 was functional in Clostridium.…”

“…Introduction of a sucrose permease from Escherichia coli and a sucrose phosphorylase from Streptococcus mutans into Bacillus subtilis resulted in a recombinant strain that showed remarkable enhancement of 2,3-butanediol production compared with the control strain [14]. Overexpression of an endogenous sucrose utilization system, the phosphoenolpyruvate-phosphotransferase system (PEP-PTS) transport protein for sucrose and the related sucrose hydrolase enzymes elevated sucrose consumption and enhanced acetone-butanol-ethanol (ABE) production in Clostridium saccharoperbutylacetonicum N1-4 [15].…”

Development of Clostridium saccharoperbutylacetonicum as a Whole Cell Biocatalyst for Production of Chirally Pure (R)-1,3-Butanediol

“…Although several native Clostridium and synthetic promoters have been used for enzyme expression in C. saccharoperbutylacetonicum ( Nakayama et al, 2008 ; Zhang et al, 2018 ) no systematic analysis of promoter strength has been published in this organism so far. A gusA (β-glucuronidase) reporter gene was employed to assess the relative strength of various clostridial promoters, including a library derived from the fdx and thl genes of several different clostridial species ( Willson, 2014 ) as well as a synthetic promoter (iGEM part BBa_J23119).…”