Enhancement of stability and catalytic activity of immobilized lipase on silica-coated modified magnetite nanoparticles

Ranjbakhsh, Elnaz; Bordbar, Abdol‐Khalegh; Abbasi, M.; Khosropour, Ahmad Reza; Shams, Esmaeil

doi:10.1016/j.cej.2011.10.097

Cited by 166 publications

(87 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The immobilized lipase conserved more than 64% of its activity after 21 days while free lipase only 47%. Also in reusing catalyst after 6 cycles, immobilized lipase retained 63% of its initial activity (Ranjbakhsh et al 2012).…”

Section: Physicochemical Properties Of Nanomaterials and Immobilized Lmentioning

confidence: 99%

“…The measurement of Michaelis-Menten parameters also revealed a considerable improvement of the immobilized lipase. In a study (Ranjbakhsh et al 2012), Km value of immobilized lipase was lower than free lipase, which represents a higher affinity of immobilized lipase to substrates. Certainly with the nano size, magnetic nanoparticles could be imagined to offer lipase molecules a porous surface with a better orientation leading to higher affinity for substrate and more available sites.…”

Section: Kinetic Parametersmentioning

confidence: 99%

“…Moreover, kinetic study showed the activation of immobilized enzyme. The enzyme recovery represents the establishment of about 64% of residual activity after six cycles of washing (Ranjbakhsh et al 2012). …”

Section: Nanoparticles As Support Materials In Immobilized Lipasementioning

confidence: 99%

See 2 more Smart Citations

Trend in enzyme immobilization on nano materials for transesterification to produce biodiesel: A review

Hindryawati¹,

Maniam²,

Feng³

et al. 2018

J bio-sci.

View full text Add to dashboard Cite

Biodiesel is a non-toxic and very low sulfur containing, renewable and biodegradable diesel fuel substitute with low volatility and high cetane number. It is derived from fresh or waste vegetable oils and animal fats transesterified with short chain alcohol such as ethanol or methanol in the presence of a catalyst. Several new types of carriers and technologies have been adopted in the recent past to improve the ability of a catalyst in transesterification. One of the new trends is nanoparticles-based immobilization of enzyme as a catalyst. The combination of the precise physical, chemical, optical and electrical properties of nanoparticles with the specific recognition site or catalytic properties has led them to appear in a myriad of novel nanomaterial application. Enzyme immobilized on nanoparticles showed a broader working temperature and pH range and thermal stability than the native enzyme. Enhancement in the reactivity of nanocatalysts is associated with their increased surface area, greater concentrations of highly reactive edge, unusual and stabilized lattice planes. The greater activity of nanomaterial immobilized biocatalyst affords operational simplicity, low energy consumption, and greater safety, in the process of transesterification. This review article highlights the issues including the exploration of the ability of nanomaterial to immobilize biocatalyst and factors that influence the activity of biocatalysts upon immobilization.

show abstract

Section: Physicochemical Properties Of Nanomaterials and Immobilized Lmentioning

confidence: 99%

Section: Kinetic Parametersmentioning

confidence: 99%

See 1 more Smart Citation

Trend in enzyme immobilization on nano materials for transesterification to produce biodiesel: A review

Hindryawati¹,

Maniam²,

Feng³

et al. 2018

J bio-sci.

View full text Add to dashboard Cite

show abstract

“…Different lipases have been used in biodiesel production, among which immobilized lipase shows great potential for industrial application due to high stability with regard to temperature and chemicals. This enables easy handling, recovery and recycling of the biocatalyst, and hence lowering the costs (Ranjbakhsh et al, 2012;Yang et al, 2013;Kalantari et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Lipase-catalyzed ethanolysis of Jatropha curcas L. oil assisted by ultrasonication

Lerin

Remonatto

Pereira

et al. 2017

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

-Transesterification of non-edible oils using immobilized lipase is a promising process for biodiesel production. Thus, this study aimed to evaluate the enzymatic transesterification of the non-edible Jatropha curcas oil for FAEE production under ultrasound irradiation in a solvent-free system. The effects of enzyme concentration, water concentration, molar ratio of ethanol to oil and ultrasound power on the FAEE conversion have been evaluated. The results show that enzyme concentration and irradiation power have a positive significant effect on FAEE conversion, where an increase in these variables leads to higher conversions. Conversion above 54% of FAEE was achieved with 1.5 hours of reaction time using ultrasound irradiation, reducing reaction time by at least 3 times, when compared with the same experimental conditions without ultrasound irradiation. Results showed that ultrasound can improve reaction conversion mainly by enhancing the mass transfer between the constituents of the reactions.

show abstract

“…Over the last decade, a variety of magnetic nanoparticles with immobilized enzymes have been adopted in several areas of application particularly for organic synthesis and bio-separation 19 . The use of magnetic nanoparticles as supports for enzyme immobilization is based on the magnetic feature that the solid phase can achieve a rapid separation by using a magnetic field, and thereby leading to the decrease of operation cost 20 . Therefore, the mesoporous SBA-15 materials modified with magnetite composites are excellent materials for the application of enzyme immobilization.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Interesterification of Soybean Oil and Methyl Stearate Blends Using Lipase Immobilized on Magnetic Fe3O4/SBA-15 Composites as a Biocatalyst

Zang

Xie

2014

J. Oleo Sci.

View full text Add to dashboard Cite

Enhancement of stability and catalytic activity of immobilized lipase on silica-coated modified magnetite nanoparticles

Cited by 166 publications

References 23 publications

Trend in enzyme immobilization on nano materials for transesterification to produce biodiesel: A review

Trend in enzyme immobilization on nano materials for transesterification to produce biodiesel: A review

Lipase-catalyzed ethanolysis of Jatropha curcas L. oil assisted by ultrasonication

Enzymatic Interesterification of Soybean Oil and Methyl Stearate Blends Using Lipase Immobilized on Magnetic Fe3O4/SBA-15 Composites as a Biocatalyst

Contact Info

Product

Resources

About