Enhancement of St. Louis Arbovirus Plaque Formation by Neutral Red

Marcovici, M.; Prier, James E.

doi:10.1128/jvi.2.3.178-181.1968

Cited by 7 publications

(1 citation statement)

References 23 publications

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“…Plaques were also observed in L-929 cells at 11 days postinfection but required a special staining technique. The basis for enhanced plaque formation in L-929 cells after early application of neutral red is unknown, but a similar observation has been made with St. Louis encephalitis virus plaque formation in duck embryo cells (5). We plan to explore this observation further to determine whether it has general applicability to rickettsial plaque formation in other cell lines.…”

Section: Discussionmentioning

confidence: 54%

Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells

Osterman

Parr

1974

Infect Immun

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Mammalian cells particularly suitable for the study of specialized aspects of rickettsial biology were tested for their ability to support plaque formation by Rickettsia conori. The detection of plaques was substantially influenced by the combination of growth medium and cell type used. Large plaques (2.0 to 3.0 mm in diameter) occurred by 8 days postinfection in WI-38 and DBS-FRhL-2 cells supported by medium 199. Smaller plaques (0.5 to 1.0 mm in diameter) were seen in L-929 and HeLa cells at 8 to 11 days postinfection and were more discernible in cells supported with Eagle minimal essential medium. Chicken embryo cells maintained in Dulbecco's modified Eagle medium exhibited large spherical plaques with a diameter of approximately 1.5 mm by 8 days postinfection.

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Section: Discussionmentioning

confidence: 54%

Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells

Osterman

Parr

1974

Infect Immun

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Establishment of duck cell line derived from experimental tumor induced by 20-methylcholanthrene

Kang

Shadduck

1977

In Vitro

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Experimental tumors developed in white Pekin ducks after intramuscular implantation of 20-methylcholanthrene. Cells derived from the primary tumor were adapted successfully to grow in vitro and have growth characteristics similar to that of established cell lines of mammalian origin. The cell density rises rapidly and the doubling time is approximately 17 hr. The duck cells have been cultured successfully for at least 80 passages in votro. The continuously cultured cells have the characteristic chromosome pattern of duck, and the DNA of the duck cell line hybridized with duck liver DNA. We believe we have established a continuous cell line of avian origin. Electron-microscopic examinations of the tumor cells and RNA-directed DNA polymerase of the cell-free supernate show no evidence of endogenous virus production.

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Species identification in cell culture: a two-pronged molecular approach

Cooper

Sykes

King

et al. 2007

In Vitro Cell.Dev.Biol.-Animal

154

124

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Species identification of cell lines and detection of cross-contamination are crucial for scientific research accuracy and reproducibility. Whereas short tandem repeat profiling offers a solution for a limited number of species, primarily human and mouse, the standard method for species identification of cell lines is enzyme polymorphism. Isoezymology, however, has its own drawbacks; it is cumbersome and the data interpretation is often difficult. Furthermore, the detection sensitivity for cross-contamination is low; it requires large amounts of the contaminant present and cross-contamination within closely related species may go undetected. In this paper, we describe a two-pronged molecular approach that addresses these issues by targeting the mitochondrial genome. First, we developed a multiplex PCR-based assay to rapidly identify the most common cell culture species and quickly detect cross-contaminations among these species. Second, for speciation and identification of a wider variety of cell lines, we amplified and sequenced a 648-bp region, often described as the "barcode region" by using a universal primer mix targeted at conserved sequences of the cytochrome C oxidase I gene (COI). This method was challenged with a panel of 67 cell lines from 45 diverse species. Implementation of these assays will accurately determine the species of cell lines and will reduce the problems of misidentification and cross-contamination that plague research efforts.

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Enhancement of St. Louis Arbovirus Plaque Formation by Neutral Red

Cited by 7 publications

References 23 publications

Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells

Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells

Establishment of duck cell line derived from experimental tumor induced by 20-methylcholanthrene

Species identification in cell culture: a two-pronged molecular approach

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