Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

Shi, Xueping; Dai, Xiaohui; Li, Guofeng; Bao, Manzhu

doi:10.1007/s00468-009-0345-9

Cited by 30 publications

(18 citation statements)

References 51 publications

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“…Though highefficiency somatic embryo formation could be induced from immature zygotic embryos by sucrose pretreatment, and mature somatic embryos could be further regenerated into plantlets in camphor tree (Shi et al 2009), complete maturation of somatic embryos is considered the major bottleneck for plant regeneration. Application of ABA treatment promoted somatic embryo maturation in camphor tree, and maturation-treated somatic embryos achieved significantly improved germination frequency (Shi et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The fruits were surface-sterilized with 0.1 % (w/v) mercuric chloride (HgCl 2 ) solution for 10 min; immature zygotic embryos were isolated from the fruits and then pretreated with 0.5 M sucrose solution for 72 h; and somatic embryos were obtained by culturing the pretreated immature zygotic embryos on induction medium for 5 weeks as previously described (Shi et al 2009). Stems, young leaves, young flowers, and young fruits were collected from the same tree during March-May.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…To address this problem, transgenic technology was applied to introduce infertile traits. A system of plant regeneration via somatic embryogenesis has been established for camphor tree (Shi et al 2009(Shi et al , 2010. Although efficient somatic embryo initiation and shoot regeneration can be induced in tissue culture, regeneration of somatic embryos after genetic transformation is recalcitrant.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning, characterization, and functional analysis of CcBBM gene from camphor tree (Cinnamomum camphora L.)

Liu

Zhang

Cheng

et al. 2015

Trees

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Key message Cloning and functional analysis of CcBBM gene. Abstract Baby boom (BBM), as a transcription factor of AP2/ERF family, has great potence in promoting somatic embryogenesis. In this study, a BBM gene from somatic embryos of camphor tree (Cinnamomum camphora L.), named CcBBM, was cloned and characterized. A partial fragment of 560 bp of CcBBM was amplified by degenerate PCR based on the two conserved AP2 domains of other annotated BBMs. Full-length cDNA of CcBBM was obtained by a cloning strategy combing degenerate PCR and RACE technique. The open-reading frame of CcBBM is 2169 bp in length, which encodes 722 amino acids. A comparison of the deduced amino acid sequence of CcBBM with BBM from other species showed high similarities, ranging from 51 to 80 %. The mRNA levels of CcBBM in different tissues were detected by semi-quantitative reverse transcription PCR (RT-PCR). CcBBM was expressed in most tissues, with highest expression level in somatic embryos, followed by roots, young fruits and flowers. Ectopic expression of CcBBM gene in Arabidopsis exhibited a variety of deformed and variegated leaves and short roots. No spontaneous somatic embryogenesis was observed in 35S::CcBBM transgenic plants. These results will help us understand the role of CcBBM in somatic embryogenesis in camphor tree.

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Section: Discussionmentioning

confidence: 99%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular cloning, characterization, and functional analysis of CcBBM gene from camphor tree (Cinnamomum camphora L.)

Liu

Zhang

Cheng

et al. 2015

Trees

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“…These factors are able to enhance the maturation and germination because they can simulate the developmental environment of ZE (Te-chato and Hilae 2007). Shi et al (2009) in studies with Cinnamomum camphora L. found that the best results for SE maturation were obtained with concentrations varying from 20 to 50 g L -1 of sucrose, while higher concentrations, 70-90 g L -1 of sucrose, resulted in a decrease in the mean number of SE.…”

Section: Synchronization and Maturation Of Somatic Embryosmentioning

confidence: 98%

An improved protocol for somatic embryogenesis and plant regeneration in macaw palm (Acrocomia aculeata) from mature zygotic embryos

Luis

Scherwinski‐Pereira

2014

Plant Cell Tiss Organ Cult

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An improved protocol for plant regeneration via somatic embryogenesis was developed using mature macaw palm (Acrocomia aculeata) zygotic embryos as initial explant. For induction of the embryogenic callus (EC), two basic media (BM) were tested consisting of Murashige and Skoog and Eeuwens (Y3) salts with 30 g L-1 sucrose, 0.5 g L-1 glutamine and 2.5 g L-1 Phytagel. The 3,6-dichloro-2-methoxybenzoic acid (dicamba), 4-amino-3,5,6-trichloro-picolinic acid (picloram) and 2,4-dichlorophenoxyacetic acid (2,4-D) auxins were added to the culture media at concentrations of 0, 1.5 or 3.0 mg L-1. After 240 days, the embryogenic calli were transferred to the respective BM media with auxin concentrations reduced to 0.5 or 1.0 mg L-1 in order to differentiate the somatic embryos (SEs). Plant regeneration was performed on the BM media without growth regulators. Embryogenic calli were observed after 180 days of culture and in all treatments with auxin. The Y3 medium showed the best EC formation results (60.8 %). These calli showed yellowish coloration, compact consistency and nodular aspect. After 60 days in differentiation medium, SEs were verified in different stages of development. Histological analysis showed that the SEs were formed from a nodular EC. The SEs generally presented unicellular origin with suspensor formation, and at the end of development, bipolar embryos were observed. The plant regeneration frequency reached levels up to 31.9 % when using induction medium consisting of Y3 associated to 1.5 mg L-1 of 2,4-D and the subsequent auxin reduction to 0.5 mg L-1 in the differentiation stage. Regenerated plants showed normal development, with root and aerial part growth.

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“…A la fecha no existen reportes de sistemas de embriogénesis somática en el género Litsea. En la familia Lauraceae hay reportes en este sentido para el laurel europeo (Laurus nobilis) (Canhoto et al, 1999), así como para los géneros Cinnamomum (Shi et al, 2009), Ocotea (Moura-Costa et al, 1993;Catarina et al, 2003) y Persea (Pliego-Alfaro, 1988).…”

Section: Introductionunclassified

REGENERACIÓN in vitro DEL LAUREL SILVESTRE (Litsea glaucescens Kunth) A TRAVÉS DE EMBRIOGÉNESIS SOMÁTICA

et al. 2016

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El laurel silvestre (Litsea glaucescens) es una de las especies forestales no maderables más importantes en México debido a su uso como condimento y a sus propiedades medicinales. Esto ha causado una sobreexplotación de muchas de las poblaciones de la especie, lo cual, junto a la degradación de su hábitat, la ha puesto en peligro de extinción. En este trabajo se reporta el desarrollo de un sistema de regeneración in vitro para esta especie a través de la embriogénesis somática indirecta. Los explantes iniciales fueron embriones cigóticos maduros, a partir de los cuales se generó tejido calloso embriogénico en medio de cultivo de Murashige y Skoog, a pH 5.7, con 30 g L-1 de sacarosa y 8 g L-1 de agar, adicionados con 4 g L-1 carbón activado o 1 mg L-1 de benciladenina. Este último fue el mejor tratamiento ya que 29 % de los explantes inoculados generó tejido embriogénico. La diferenciación de los embriones somáticos se observó en medio de cultivo con 4 g L-1 de carbón activado, con una producción promedio de 282 embriones somáticos por cada gramo de tejido embriogénico. La maduración y germinación de los embriones somáticos fue más eficiente (27 %) en medio basal con 6 g L-1 de carbón activado y 12 g L-1 de agar. La conversión de embriones germinados en plántulas se logró en medio basal a 50 % con 8 g L-1 de agar, esto con una eficiencia de 87 %. Finalmente, 76 % de las plantas generadas a través de la embriogénesis somática sobrevivieron y reiniciaron su crecimiento en suelo.

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Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

Cited by 30 publications

References 51 publications

Molecular cloning, characterization, and functional analysis of CcBBM gene from camphor tree (Cinnamomum camphora L.)

Molecular cloning, characterization, and functional analysis of CcBBM gene from camphor tree (Cinnamomum camphora L.)

An improved protocol for somatic embryogenesis and plant regeneration in macaw palm (Acrocomia aculeata) from mature zygotic embryos

REGENERACIÓN in vitro DEL LAUREL SILVESTRE (Litsea glaucescens Kunth) A TRAVÉS DE EMBRIOGÉNESIS SOMÁTICA

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