Enhancement of somatic embryogenesis frequency by gibberellic acid in fennel

Hunault, G.; Maatar, Abdelaziz

doi:10.1007/bf00051587

Cited by 16 publications

(9 citation statements)

References 20 publications

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“…We also found that GA 3 increased somatic embryo production in sugar beet callus. GA 3 enhanced somatic embryogenesis from Rumex acetosella callus (Culafic et al 1987), Spinacia oleracea callus (Xiao and Branchard 1993) and Foeniculum vulgare petiole (Hunault and Maatar 1995), and the germination of somatic embryos into plantlets (Culafic et al 1987). GA 3 may act on somatic embryo development or the induction of embryogenic competence.…”

Section: Discussionmentioning

confidence: 99%

Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines

Zhang

Chen

Kubaláková

et al. 2008

Plant Cell Tiss Organ Cult

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Efficient regeneration via somatic embryogenesis (SE) would be a valuable system for the micropropagation and genetic transformation of sugar beet. This study evaluated the effects of basic culture media (MS and PGo), plant growth regulators, sugars and the starting plant material on somatic embryogenesis in nine sugar beet breeding lines. Somatic embryos were induced from seedlings of several genotypes via an intervening callus phase on PGo medium containing N 6 -benzylaminopurine (BAP). Calli were mainly induced from cotyledons. Maltose was more effective for the induction of somatic embryogenesis than was sucrose. There were significant differences between genotypes. HB 526 and SDM 3, which produced embryogenic calli at frequencies of 25-50%, performed better than SDM 2, 8, 9 and 11. The embryogenic calli and embryos produced by this method were multiplied by repeated subculture. Histological analysis of embryogenic callus cultures indicated that somatic embryos were derived from single-or a small number of cells. 2,4-dichlorophenoxyacetic acid (2,4-D) was ineffective for the induction of somatic embryogenesis from seedlings but induced direct somatic embryogenesis from immature zygotic embryos (IEs). Somatic embryos were mainly initiated from hypocotyls derived from the cultured IEs in line HB 526. Rapid and efficient regeneration of plants via somatic embryogenesis may provide a system for studying the molecular mechanism of SE and a route for the genetic transformation of sugar beet.

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Section: Discussionmentioning

confidence: 99%

Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines

Zhang

Chen

Kubaláková

et al. 2008

Plant Cell Tiss Organ Cult

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“…Literature reports that in fennel shoot formation occurred only from a ramification of the buds used as primary explants or via embryogenesis (Paupardin et al, 1980;Hunault et al, 1989;Hunault and Maatar, 1995). Instead, in our material, maintained in presence of NAA and kinetin, plant regeneration via organogenesis from callus was induced.…”

mentioning

confidence: 86%

“…al., 1985), establishment of callus (Paupardin et al, 1980;Hunault, 1981), suspension cultures (Hunault, 1984) and the capacity to produce plantlets via somatic embryogenesis in vitro (Maheshwari and Gupta, 1965;Hunault, 1984;Theiler-Hedtrich and K~igi, 1991;Hunault and Maatar, 1995).…”

mentioning

confidence: 99%

In vitro culture of Foeniculum vulgare: callus characteristics in relation to morphogenesis

Anzidei

Vivona

Schiff

et al. 1996

Plant Cell Tiss Organ Cult

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“…About 1 to 2 weeks after explants exposure on callus induction medium, they significantly swallowed (especially in areas of cut (2009) results. Hunault & maatar(1995)and Miura & et al(1987) could receive appropriate response of fennel petioles as explants. Anzidei &et al (2000) worked in Fennel hypocotyl in laboratory and also in the present study had obtained favorable results of fennel petiole and hypocotyls in tissue culture environment.…”

Section: Resultsmentioning

confidence: 99%

A survey of the best growing conditions of in vitro culture in fennel different genotypes for mass production

Torabi¹,

Far²,

Omidi³

et al. 2014

Int. J. Biosci.

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To determine the best growing conditions for in vitro culture of different genotypes of fennel to mass production, we compared different explants in various culture mediums for eight fennel genotype groups. Based on completely randomized plan, factorial experiment was done with four replications. Leaf, petiole, hypocotyl and root explants were isolated for 20 days, and seedlings were cultured on callus induction medium, 1/2 MS basal medium with 2,4-dichlorophenoxyacetic acid (2, 4-D) (2, 4 and 6 mg l -1 ) and 6-benzyaminopurine (BAP) (0.5, 0.25 and 0.1 mg l -1 ) combination. Color, Size, Percentage of callus induction, dry and fresh weight and Volume of callus were evaluated 3 month later. Through most of the genotypes, Hypocotyls and petiole explants in callus induction were better than the others. Callus induction mediums with 6 mgl -1 2-4-D and BAP combinations had more appropriate responses. Leaf explants showed the highest callus size in the German genotype in callus induction medium with 4mgl -1 2-4-D and 0.1mgl -1 BAP. Embryogenic calli were placed on regeneration medium with 1/2MS of a non-hormonal treatment and hormonal treatment with a level of 2-4-D (10 mgl -1 ) and two levels of BAP (2 and 5 mgl -1 ). The medium without hormones was recognized as the best medium for regeneration and hypocotyl and petiole had the highest rate of regeneration. Respectively, Tabriz and Gonabad genotypes were shown highest and lowest rate of regeneration.

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Enhancement of somatic embryogenesis frequency by gibberellic acid in fennel

Cited by 16 publications

References 20 publications

Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines

Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines

In vitro culture of Foeniculum vulgare: callus characteristics in relation to morphogenesis

A survey of the best growing conditions of in vitro culture in fennel different genotypes for mass production

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