Enhancement of somatic embryogenesis and plant regeneration in Japanese red pine (Pinus densiflora)

Kim, Yong-Wook; Moon, Heung-Kyu

doi:10.1007/s11816-014-0319-2

Cited by 34 publications

(19 citation statements)

References 27 publications

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“…In general, the extrusion of ET occurred 2–6 weeks after culture, and the proliferation of ET became obvious after 4–8 weeks of culture when EM increased (Maruyama and Hosoi, 2014). The induction frequency on the medium supplemented with PGRs varied from 1.0 to 2.8%, with an overall average of 2.3% (Maruyama et al, 2005a,b, 2007; Hosoi and Maruyama, 2012), which was comparable with previous results for P. densiflora (up to 2.3%, Kim and Moon, 2014), P. nigra (3.1%, Salajová and Salaj, 2005), Pinus banksiana (up to 3.9%, Park et al, 2006), P. rigida × P. taeda (up to 1.1%, Kim and Moon, 2007b), and P. lambertiana (1–3%, Gupta, 1995). These results were inconsistent with the high induction rates reported for P. radiata (44–93%, Hargreaves et al, 2011), P. taeda (up to 79%, Gupta, 2014), P. strobus (54%, Finer et al, 1989), Pinus sylvestris (up to 30%, Aronen et al, 2009), and P. pinaster (up to 75%, Park et al, 2006).…”

Section: Induction and Proliferation Of Etsupporting

confidence: 88%

“…At present, the application of PEG with ABA has become the most used and successful procedure for promoting embryo development in several genera of conifers (Stasolla and Yeung, 2003). Similar to JPs, the beneficial effect of AC has been widely reported in other conifer trees (Jain et al, 1995b; Maruyama et al, 2000, 2002, 2005c; Kim and Moon, 2014; Ahn et al, 2017). The positive effect of AC may be related to the capture of residual PGRs, undesirable substances, and other toxic metabolites present in the culture medium (Pan and van Staden, 1998).…”

Section: Maturation Of Somatic Embryossupporting

confidence: 52%

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Progress in Somatic Embryogenesis of Japanese Pines

Maruyama

Hosoi

2019

Front. Plant Sci.

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Somatic embryogenesis (SE) in not only one of the most promising techniques for mass propagation of selected trees, but also is a valuable tool for basic research studies in cell biology and genetic engineering, and it allows the long-term ex situ conservation of genetic resources by cryopreservation techniques. This review reports the most recent progress in SE, protoplast culture, and cryopreservation of four important Japanese pines (Pinus thunbergii, Pinus densiflora, Pinus armandii var. amamiana, and Pinus luchuensis). Induction of embryogenic tissues (ET), embryogenic culture maintenance/proliferation, production of somatic embryos, germination, and conversion to plants are described focusing on the protocols most commonly reported for plant production in Pinus species through to SE.

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Section: Induction and Proliferation Of Etsupporting

confidence: 88%

Section: Maturation Of Somatic Embryossupporting

confidence: 52%

Progress in Somatic Embryogenesis of Japanese Pines

Maruyama

Hosoi

2019

Front. Plant Sci.

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“…However, the responses vary according to plant species and growth stages. Monochromatic red light can promote the germination of somatic embryos of Pinus densiflora [6]. Kwon et al [7] pointed out that R/B (1:1) can promote cell division of Populus euramericana on in vitro culture than monochromatic light or fluorescence.…”

Section: Introductionmentioning

confidence: 99%

Effects of LED photoperiods and light qualities on in vitro growth and chlorophyll fluorescence of Cunninghamia lanceolata

Yang

Cheng

et al. 2020

BMC Plant Biol

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Background: Cunninghamia lanceolata (C. lanceolata) is the main fast-growing timber species in southern China. As an alternative to conventional lighting systems, LED has been demonstrated to be an artificial flexible lighting source for commercial micropropagation. The application of LED can provide rapid propagation of C. lanceolata in vitro culture. Results: We applied two-factor randomized block design to study the effects of LED photoperiods and light qualities on the growth and chlorophyll fluorescence of C. lanceolata in vitro culture plantlets. In this study, plantlets were exposed to 20 μmol•m − 2 •s − 1 irradiance for three photoperiods, 8, 16, and 24 h under the three composite lights, 88.9% red+ 11.1% blue (R/B), 80.0% red+ 10.0% blue+ 10.0% purple (R/B/P), 72.7% red+ 9.1% blue+ 9.1% purple+ 9.1% green (R/B/P/G), as well as white light (12.7% red+ 3.9% blue+ 83.4% green, W) as control. The results showed that: (1) Plant height, dry weight, rooting rate, average root number, length, surface area and volume, chlorophyll, and chlorophyll fluorescence parameters were significantly affected by photoperiods, light qualities and their interactions. (2) Plantlets subjected to photoperiod 16 h had longer root, higher height, rooting rate, root number, and the higher levels of chlorophyll, chlorophyll a/b, Y (II), qP, NPQ/4 and ETR II compared to photoperiods 8 h and 24 h, while Fv/Fm during photoperiod 16 h was lower than 8 h and 24 h. Plantlets exposed to R/B/P/G generated more root and presented higher chlorophyll, Fv/Fo, Y (II), qP, and ETR II than W during photoperiods 8 and 16 h. (3) Total chlorophyll content and ETR II were significant correlated with rooting rate, root length and root volume, while Fv/Fm and ETR II were significant correlated with plant height, average root number and root surface area. (4) 16-R/B/P/G is best for growing C. lanceolata plantlets in vitro. Conclusions: This study demonstrated the effectiveness of photoperiods and light qualities using LEDs for micropropagation of C. lanceolata. The best plantlets were harvested under 16-R/B/P/G treatment. And there was a correlation between the growth and the chlorophyll and chlorophyll fluorescence of their leaves under different photoperiod and light quality. These results can contribute to improve the micropropagation process of this species.

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“…Somatic embryo production obtained in this study was higher than those previously reported for sugi in 2000 (up to 67 embryos per plate) [ 33 ] and 2003 (up to 46 embryos per plate) [ 36 ], but similar to the results published in 2007 (up to 361 embryos per plate) [ 39 ]. For comparison, the numbers of cotyledonary embryos produced per gram of embryogenic cells in studies of other conifer trees have been reported as 68–147 for Pinus strobus [ 46 ], 2–441 for P. sylvestris [ 60 ], 10–1550 for P. radiata [ 61 ], 0–798 for P. densiflora [ 62 ], 67–551 for Larix leptolepis [ 63 ], 8–1566 for hybrid Larix × eurolepis [ 64 ], 80–200 for Picea abies [ 65 ], and 22–925 for Abies fraseri [ 66 ].…”

Section: Resultsmentioning

confidence: 99%

Somatic Embryogenesis and Plant Regeneration from Sugi (Japanese Cedar, Cryptomeria japonica D. Don, Cupressaceae) Seed Families by Marker Assisted Selection for the Male Sterility Allele ms1

Maruyama

Ueno

Hirayama

et al. 2020

Plants

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One of the possible countermeasures for pollinosis caused by sugi (Cryptomeria japonica), a serious public health problem in Japan, is the use of male sterile plants (MSPs; pollen-free plants). However, the production efficiencies of MSPs raised by conventional methods are extremely poor, time consuming, and resulting in a high seedling cost. Here, we report the development of a novel technique for efficient production of MSPs, which combines marker-assisted selection (MAS) and somatic embryogenesis (SE). SE from four full sib seed families of sugi, carrying the male sterility gene MS1, was initiated using megagametophyte explants that originated from four seed collections taken at one-week intervals during the month of July 2017. Embryogenic cell lines (ECLs) were achieved in all families, with initiation rates varying from 0.6% to 59%. Somatic embryos were produced from genetic marker-selected male sterile ECLs on medium containing maltose, abscisic acid (ABA), polyethylene glycol (PEG), and activated charcoal (AC). Subsequently, high frequencies of germination and plant conversion (≥76%) were obtained on plant growth regulator-free medium. Regenerated plantlets were acclimatized successfully, and the initial growth of male sterile somatic plants was monitored in the field.

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Enhancement of somatic embryogenesis and plant regeneration in Japanese red pine (Pinus densiflora)

Cited by 34 publications

References 27 publications

Progress in Somatic Embryogenesis of Japanese Pines

Progress in Somatic Embryogenesis of Japanese Pines

Effects of LED photoperiods and light qualities on in vitro growth and chlorophyll fluorescence of Cunninghamia lanceolata

Somatic Embryogenesis and Plant Regeneration from Sugi (Japanese Cedar, Cryptomeria japonica D. Don, Cupressaceae) Seed Families by Marker Assisted Selection for the Male Sterility Allele ms1

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