Enhancement of soluble CD23 serum levels and cell-surface CD23-expression in subjects at increased risk of Type 1 diabetes mellitus and in diabetic patients

Avanzini, Maria Antonietta; Vitali, Letizia; d’Annunzio, Giuseppe; Amici, Mara De; Strigazzi, C.; Alibrandi, A.; Lorini, Renata

doi:10.1002/(sici)1096-9136(199804)15:4<320::aid-dia563>3.0.co;2-i

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…Differences in the stage of the autoimmune process cannot contribute to contrasting results from Avanzini et al . [19], as speculated [16]. Indeed, the duration of disease in our study covers a very wide range, yet we found no association with CD23 expression.…”

Section: Discussionsupporting

confidence: 66%

Redox status and immune function in type I diabetes families

Matteucci¹,

Malvaldi

Fagnani³

et al. 2004

Clinical and Experimental Immunology

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SUMMARYBecause abnormalities in redox balance cluster in type I diabetes families and the intracellular thiol redox status seems to modulate immune function, we aimed to investigate the relationship between oxidative stress and immunological features. We measured oxidative markers, serum proinflammatory cytokines, soluble cytokine receptors and subsets of peripheral blood lymphocytes (by varying combinations of CD4, CD8, CD23 or low-affinity IgE receptor, and CD25 or IL-2 receptor) from 38 type I patients, 76 low-risk (i.e. without underlying islet autoimmunity) non-diabetic first-degree relatives of diabetic patients, and 95 healthy subjects. In type I diabetes families, protein and lipid oxidation was confirmed by the presence of reduced sulphhydryl groups, increased advanced oxidation protein products, and increased plasma and erythrocyte malondialdehyde. Relatives had decreased counts of monocytes, of cells co-expressing CD23 and CD25 and of CD25 + cells in peripheral blood. Patients with TIDM had similar defects and, in addition, showed decreased counts of peripheral CD4 + CD8+ lymphocytes and increased serum levels of soluble receptors for interleukin (IL)-6 and IL-2. Abnormal indicators of oxidative stress were related in part to immune abnormalities. In the whole study group, we found a correlation (multiple R 0·5, P < 0·001) of CD23 + CD25+ cells with blood counts of monocytes, CD4 + CD8 + cells, CD25 + cells, basal haemolysis and plasma levels of thiols. In type I diabetics, anti-GAD65 antibody levels were associated (multiple R 0·6, P = 0·01) positively with sIL-6R, negatively with duration of diabetes and CD23 + CD25 + counts; plasma creatinine correlated positively (multiple R 0·6, P < 0·001) with both sIL-2R and tumour necrosis factor (TNF)-a concentration. Our study reports the first evidence that the oxidative stress observed in type I families is related to immunological hallmarks (decreased peripheral numbers of monocytes as well as cells bearing a CD4 + CD8 + , CD23 + CD25 + and CD25 + phenotype) from which the involvement of some immunoregulatory mechanisms could be suspected. It remains to be elucidated the course of events culminating in the loss of physiological immune homeostasis and disease pathology.

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Section: Discussionsupporting

confidence: 66%

Redox status and immune function in type I diabetes families

Matteucci¹,

Malvaldi

Fagnani³

et al. 2004

Clinical and Experimental Immunology

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Circulating L-Selectin Concentrations in Children with Recent-Onset IDDM

Kordonouri¹,

Bührer²

2000

Journal of Pediatric Endocrinology and Metabolism

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To clarify conflicting claims of altered serum concentrations of soluble L-selectin (sCD62L) in recent-onset IDDM, sCD62L was measured in 89 children and adolescents with IDDM (35 recent-onset, 12 during the first year of insulin treatment, and 42 with long-standing (> 1 yr) treatment) alongside 124 controls. Children < 14 yr of age both with and without IDDM (n = 160) had grossly elevated sCD62L concentrations (20.2 +/- 4.9 nmol/l), as compared with adolescents (14-18 yr, n = 23; 15.9 +/- 3.9 nmol/l) and adults (> 18 yr, n = 30; 11.2 +/- 2.3 nmol/l) (p < 0.0001). Multivariate analysis confirmed the strong inverse association between age and sCD62L (p < 0.001) while revealing that sCD62L concentrations were slightly elevated in recent-onset IDDM, as compared with insulin-treated IDDM patients or nondiabetic controls (p = 0.028). Actual sCD62L concentrations in the 35 recent-onset IDDM patients were 22.2 +/- 4.9 nmol/L vs 19.6 +/- 3.6 nmol/l in 35 non-diabetic controls matched for age (p = 0.022). While this significant but small rise of systemic sCD62L reflects leukocyte activation, it is obscured by the inverse association between sCD62L and chronological age in children and adolescents. Therefore, determining sCD62L serum concentrations appears to be of limited value for clinical investigators caring for children and adolescents with IDDM.

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Enhancement of soluble CD23 serum levels and cell-surface CD23-expression in subjects at increased risk of Type 1 diabetes mellitus and in diabetic patients

Cited by 2 publications

References 14 publications

Redox status and immune function in type I diabetes families

Redox status and immune function in type I diabetes families

Circulating L-Selectin Concentrations in Children with Recent-Onset IDDM

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