Enhancement of salt tolerance in transgenic rice expressing an Escherichia coli catalase gene, katE

Nagamiya, Kenji; Motohashi, Tsuyoshi; Nakao, Kimiko; Prodhan, Shamsul H.; Hattori, Eriko; Hirose, Sakiko; Ozawa, Kenjiro; Ohkawa, Yasunobu; Takabe, Tetsuko; Takabe, Teruhiro; Komamine, Atsushi

doi:10.1007/s11816-007-0007-6

Cited by 69 publications

(30 citation statements)

References 32 publications

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“…Sudhakar et al, (2001) have shown that CAT activity increases by more in salt tolerant mulberry cultivars $1 than salt susceptible cultivar ATP at higher concentration of NaCI. The Escherichia coli catalase encoded by the kate gene overexpressed under CaMV35S promoter in Japonica rice (Oryza sativa) conferred transgenic rice plants tolerance to the salt and catalase activity being about 1-5 to 2.5 times more than non-transgenic plants (Nagamiya et al, 2007). sion of antioxidant defence enzymes in transgenic plants (Lee et al, 2007).…”

Section: Antioxidants In Plantsmentioning

confidence: 99%

Reactive oxygen species, antioxidants and signaling in plants

2008

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Several reactive oxygen species (ROS) are continuously produced in plants as byproducts of many metabolic reactions, such as photosynthesis, photorespiration and respiration. Depending on the nature of the ROS species, some are highly toxic and rapidly detoxified by various cellular enzymatic and nonenzymatic mechanisms. Oxidative stress occurs when there is a serious imbalance between the production of ROS and antioxidative defence. ROS participate in signal transduction, but also modify cellular components and cause damage. ROS is highly reactive molecules and can oxidize all types of cellular components. Various enzymes involved in ROS-scavenging have been manipulated and over expressed or down regulated. An overview of the literature is presented in terms of primary antioxidant free radical scavenging and redox signaling in plant cells. Special attention is given to ROS and ROS-anioxidant interaction as a metabolic interface for different types of signals derived from metabolisms and from the changing environment.

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Section: Antioxidants In Plantsmentioning

confidence: 99%

Reactive oxygen species, antioxidants and signaling in plants

2008

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“…Hence, different attempts have been made to overexpress catalase genes in plants with the aim to increase plant stress resistance. For example, introduction of KatE from E. coli into tobacco and rice resulted in high tolerance to drought and salinity in the crops, respectively [22][23][24]. Transgenic Arabidopsis containing the broccoli catalase gene BoCAT showed heat tolerance by removing the H 2 O 2 accumulated due to high temperature [25].…”

Section: Introductionmentioning

confidence: 99%

CsCAT3, a catalase gene fromCucumis sativus, confers resistance to a variety of stresses toEscherichia coli

Zhou

Liu

Yang

et al. 2017

Biotechnology & Biotechnological Equipment

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Catalase (CAT) is a key scavenging enzyme for the degradation of hydrogen peroxide (H 2 O 2 ) and plays an important role in the tolerance to diverse abiotic stresses in many different organisms. In this study, we characterized the function of a catalase gene (CsCAT3) previously isolated from Cucumis sativus in the defence against a variety of stresses. Protein alignment and phylogenetic analysis revealed that CsCAT3 was clustered in the dicot group and shared 41%-95% identity with other plant CATs. Expression analyses revealed that the expression of CsCAT3 was induced by diverse abiotic stresses such as heat, polyethylene glycol, cold and NaCl treatment, as well as by signalling molecules such as abscisic acid (ABA) and H 2 O 2 . An Escherichia coli heterologous expression system was constructed to characterize the function of CsCAT3 in vitro. Its overexpression in E. coli could increase the tolerance to heat, cold, salinity and osmotic conditions. These results indicate that CsCAT3 plays important roles in abiotic stress tolerance and that CsCAT3 confers tolerance in E. coli recombinants against abiotic stresses, which may be due to the increased activities of antioxidant enzymes that reduce the oxidative damage caused by stress conditions.

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“…However, plant's capacity to effi ciently metabolize has been extensively reported to be signifi cantly improved by overexpressing a range of genes associated with ROS scavenging/detoxifying enzymes including aldehyde dehydrogenases (ALDHs) (Nakazono et al 2000 ), Mn-superoxide dismutase (Mn-SOD) (McKersie et al 1996 ;Gusta et al 2009 ;Waterer et al 2010 ), ascorbate peroxidases (APX) (Wang et al 2006 ), and catalase (CAT) (Nagamiya et al 2007 ). In transgenic rice plants, Zhao and Zhang ( 2006 ) constitutively co-expressed glutathione-S -transferase (GST) and CAT genes and achieved improved tolerance to salinity-caused oxidative stresses (Zhao and Zhang 2006 ).…”

Section: Detoxifying Reactive Oxygen Speciesmentioning

confidence: 99%

Abiotic Stress Tolerance and Sustainable Agriculture: A Functional Genomics Perspective

Gill¹,

Anjum

Gill³

et al. 2015

Elucidation of Abiotic Stress Signaling in Plants

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Crop growth and productivity is being seriously constrained by a range of abiotic stress factors all over the globe. Literature revealed that abiotic stress factors [temperature extremes (heat and cold), water extremes (drought and fl ooding), salinity, sodicity, wounding, metal/metalloid toxicity, excess light, radiations, high speed wind, nutrient loss, and anaerobic conditions] are the key reason for declining the usual yield of major crop plants by more than 50 %, which causes signifi cant economic losses every year. A number of genes and their products respond to abiotic stress factors at transcriptional and translational level; therefore, genetic engineering for abiotic stress resistance is an important goal for protecting/improving agricultural crop productivity. Adaptation of plants to various environmental insults is reliant upon the establishment of cascades of molecular networks involved in stress perception, signal transduction, and the expression of stress-specifi c genes and metabolites. Thus, engineering stress-responsive genes which can protect and/ or preserve the function may be a potential target to enhance stress tolerance in plants. Genetic engineering and DNA markers have now emerged as important gear in crop improvement.

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Enhancement of salt tolerance in transgenic rice expressing an Escherichia coli catalase gene, katE

Cited by 69 publications

References 32 publications

Reactive oxygen species, antioxidants and signaling in plants

Reactive oxygen species, antioxidants and signaling in plants

CsCAT3, a catalase gene fromCucumis sativus, confers resistance to a variety of stresses toEscherichia coli

Abiotic Stress Tolerance and Sustainable Agriculture: A Functional Genomics Perspective

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