Blockade interactions whereby a single particle prevents the flow or excitation of other particles provide a mechanism for control of quantum states, including entanglement of two or more particles. Blockade has been observed for electrons 1-3 , photons 4 and cold atoms 5 . Furthermore, dipolar interactions between highly excited atoms have been proposed as a mechanism for 'Rydberg blockade' 6,7 , which might provide a novel approach to a number of quantum protocols [8][9][10][11] . Dipolar interactions between Rydberg atoms were observed several decades ago 12 and have been studied recently in a many-body regime using cold atoms [13][14][15][16][17][18] . However, to harness Rydberg blockade for controlled quantum dynamics, it is necessary to achieve strong interactions between single pairs of atoms. Here, we demonstrate that a single Rydberg-excited rubidium atom blocks excitation of a second atom located more than 10 µm away. The observed probability of double excitation is less than 20%, consistent with a theoretical model of the Rydberg interaction augmented by Monte Carlo simulations that account for experimental imperfections.The mechanism of Rydberg blockade is shown in Fig. 1a. Two atoms, one labelled 'control' and the other 'target', are placed in proximity with each other. The ground state |1 and Rydberg state |r of each atom form a two-level system that is coupled by laser beams with Rabi frequency Ω . Application of a 2π pulse (Ωt = 2π with t being the pulse duration) on the target atom results in excitation and de-excitation of the target atom giving a phase shift of π on the quantum state, |1 t → −|1 t . If the control atom is excited to the Rydberg state before application of the 2π pulse, the dipole-dipole interaction |r c ↔ |r t shifts the Rydberg level by an amount B that detunes the excitation of the target atom so that it is blocked and |1 t → |1 t . Thus, the excitation dynamics and phase of the target atom depend on the state of the control atom. Combining this Rydberg-blockade-mediated controlled-phase operation 6 with π/2 single-atom rotations between states |0 t and |1 t of the target will implement the CNOT gate between two atoms. We have previously demonstrated the ability to carry out ground-state rotations at individual trapping sites 19 , as well as coherent excitation from ground to Rydberg states at a single site 20 . Here, we describe experiments that demonstrate the Rydberg blockade effect between two neutral atoms separated by more than 10 µm, which is an enabling step towards creation of entangled atomic states. Previous demonstrations of neutral-atom entanglement have relied on shortrange collisions at length scales characterized by a low-energy scattering length of about 10 nm (refs 21,22). Our results, using laser-cooled and optically trapped 87 Rb, extend the distance for strong two-atom interactions by three orders of magnitude, and place us in a regime where the interaction distance is large compared with 1 µm, which is the characteristic wavelength of light needed for...