Enhancement of recombinant human BMP‐7 bone formation with bmp binding peptide in a rodent femoral defect model

Abstract

Bone morphogenetic binding peptide (BBP) is an 18.5 kDa fragment of a bone matrix protein peptide. A rat femoral defect model was used to test the effect of BBP combined with recombinant human bone morphogenetic protein-7 (rhBMP-7) to induced bone healing. Two doses of BBP (500 and 1000 mg) were tested with two doses of rhBMP-7 (2 and 5 mg), and the results were compared with a positive control (10 mg rhBMP-7). Bone healing was evaluated by radiology, manual palpation, microcomputed tomography, and histology. The high dose of 10 mg of rhBMP-7 resulted in a consistent 100% bone union rate and a mature histological appearance on histology, and was used as a positive control. When 1000 mg of BBP was combined with lower doses of BMP-7 (2 mg rhBMP-7 or 5 mg rhBMP-7) significant differences were seen in radiographic scores, manual palpation, and bone volume, when compared to 2 mg rhBMP-7 or 5 mg rhBMP-7 alone. The combination of 1000 mg of BBP and 5 mg rhBMP-7 also achieved 100% fusion rate, induced a larger amount of bone formation, and yielded similar maturity of bone marrow when compared with the high dosage 10 mg rhBMP-7 group. This study demonstrated that when combined together, BBP can enhance the bone healing of rhBMP-7. Improved healing imparted by the addition of BBP may result in lesser amounts of rhBMP-7 needed to achieve union in the clinical setting. ß

Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique

“…Twelve weeks after the second operation, rat femurs were collected for X‐ray assessment and manual palpation. All defects of the harvested femurs were palpated after removal of the stainless steel plate (Liao et al ., ). Any gap in the defect was considered to be non‐union.…”

Cooperative effects in differentiation and proliferation between PDGF-BB and matrix derived synthetic peptides in human osteoblasts

“…Enhancing the osteogenic capabilities of bone matrix for the treatment of fractures and segmental bone defects is an active area of research. It is well-known that the use of oligopeptides or growth factors promotes osteogenic cell proliferation and differentiation in vitro and in vivo [ 1 - 4 ]. There are many different pathways in research to address the acceleration of fracture healing.…”

BMP-2 Promotes Bone Formation in Bone Defects in Which Bone Remodeling Is Suppressed by Long-term and High-dose Zoledronic Acid

“…All animals were cared for in accordance with the regulations of the IACUC under the supervision of licensed veterinarians. The surgical procedures and assessment of the unions were performed according to the methods of Liao et al [13].…”

“…Bone morphogenetic proteins (BMPs) are a subgroup of the transforming growth factor-β (TGF-β) superfamily [13,14,15]. Many studies have reported that BMPs can accelerate bone regeneration and fracture healing [14,16].…”