Enhancement of recombinant adeno-associated virus mediated transgene expression by targeted echo-contrast agent

Yang, Shaoling; Mu, Yi Ming; Tang, Ke; Jiang, Xueyou; Bai, Wen-Tao; Shen, Enlong; Hu, Bo

doi:10.4238/2013.april.25.3

Cited by 4 publications

(4 citation statements)

References 35 publications

(35 reference statements)

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“…Insomnia is one of the most common reasons for referral to mental health services in deployed military personnel (Cozza et al, 2004). After returning home, as many as 70% of Service Members continue to report chronic and clinically significant sleep complaints, including insomnia (Collen, Orr, Lettieri, Carter, & Holley, 2012; McLay, Klam, & Volkert, 2010; Mysliwiec et al, 2013; Seelig et al, 2010; Wright, Adler, Bliese, & Eckford, 2008; Yang et al, 2013). These observations are consistent with prior studies of Vietnam Veterans (Lewis, Creamer, & Failla, 2009; Neylan et al, 1998) and Veterans of the first Gulf War (Kroenke, Koslowe, & Roy, 1998), showing that insomnia does not spontaneously remit over time in deployed and/or combat-exposed Service Members.…”

Section: Introductionmentioning

confidence: 99%

Treatment for insomnia in combat-exposed OEF/OIF/OND Military Veterans: Preliminary randomized controlled trial

Germain

Richardson

Stocker

et al. 2014

Behaviour Research and Therapy

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Objective Chronic insomnia is highly prevalent among military personnel returning from Iraq and Afghanistan. We evaluated the effects of a military version of a brief behavioral treatment of insomnia (BBTI-MV) compared to an information only control (IC) condition in combat-exposed Veterans of Operations Enduring/Iraqi Freedom or Operation New Dawn (OEF/OIF/OND) on insomnia, sleep quality, and daytime symptoms of anxiety and depression. Method Forty OEF/OIF/OND Veterans (Mean age = 38.4 years old, s.d. = 11.69; 85% men; 77.5% white) were randomized to one of two conditions. BBTI-MV consisted of two in-person sessions and two telephone contacts delivered over four weeks, and included personalized recommendations to reduce insomnia. The IC condition also consisted of 2 in-person sessions two telephone contacts delivered over four weeks, and Veterans were encouraged to read written information about sleep-promoting behaviors. The Insomnia Severity Index, Pittsburgh Sleep Quality Index, PTSD Checklist, and Beck Depression and Anxiety Inventories were completed at baseline, post-treatment, and at the six-month follow-up. Results Both interventions were associated with clinically significant improvements in insomnia, although the magnitude of improvements in sleep and rates of treatment response and remission were greater for BBTI-MV compared to IC from pre- to post-treatment. Conclusion Both BBTI-MV and the provision of information were associated with clinically significant improvements in insomnia among Veterans. Despite the preliminary nature of the findings and limitations inherent to small controlled trials, the findings suggest that both approaches may provide viable options in a stepped-care approach to the treatment of insomnia in retuning combat-exposed Veterans. Larger, confirmatory effectiveness trials are required.

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Section: Introductionmentioning

confidence: 99%

Treatment for insomnia in combat-exposed OEF/OIF/OND Military Veterans: Preliminary randomized controlled trial

Germain

Richardson

Stocker

et al. 2014

Behaviour Research and Therapy

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“…UTMd could be a potential tailored therapy used to induce cardiac regeneration following an extensive infarct, which is able to deliver repeated regimens until ventricular function and myocardial perfusion have been restored (40). A previous study demonstrated that systemic administration of UTMd can improve the targeted gene transfer to the heart (41). Furthermore, increasing evidence has indicated that UTMd increases the migration of BMScs to the injured kidney and infarcted myocardium (42,43).…”

Section: Discussionmentioning

confidence: 99%

Ultrasound‑targeted microbubble destruction‑mediated Galectin‑7‑siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats

et al. 2020

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Bone marrow mesenchymal stem cells (BMSCs) are accepted as a form of cellular therapy to improve cardiac function following acute myocardial infarction (AMI). The present study was performed to investigate the synergistic effect of ultrasound-targeted microbubble destruction (UTMD)-mediated Galectin-7-small interfering (si)RNA with the homing of BMSCs for AMI. The rat model of AMI was established, followed by identification of BMSCs. Rats with AMI received BMSC transplantation, BMSC transplantation + UTMD + siRNA negative control, or BMSC transplantation + UTMD + Galectin-7-siRNA. The cardiac function, hemodynamics indexes, degree of myocardial fiber injury and expression of apoptosis-related proteins in myocardial tissues of rats were detected. The homing of BMSCs was observed, and the indexes of myocardial microenvironment and the TGF-β/Smads pathway-related proteins in myocardial tissues were determined. AMI rats treated with UTMD-mediated Galectin-7-siRNA exhibited improved cardiac function and hemodynamics-related indices, decreased myocardial fiber injury and apoptotic cells, as well as enhanced homing ability of BMSCs, improved myocardial microenvironment, and suppressed TGF-β1/Smads pathway activation. In conclusion, the present study demonstrated that UTMD-mediated Galectin-7-siRNA treatment could enhance the homing ability of BMSCs, thus alleviating AMI in rats.

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“…The software automatically recorded the refill time and the intensity curve of the contrast agent. Based on this analysis, the values of A (plateau intensity with respect to the myocardial blood volume), β (refill rate with respect to the velocity of myocardial blood flow), and A × β (with respect to myocardial blood flow) as well as the fitting function Y = A × (1 − e −βt ) were determined ( Figure 2 ) (Yang et al, 2013).…”

Section: Ami Animal Modelmentioning

confidence: 99%

In Vivo Ultrasound Molecular Imaging of SDF-1 Expression in a Swine Model of Acute Myocardial Infarction

Wang

Yang

et al. 2019

Front. Pharmacol.

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Background: Stem cell therapy of acute myocardial infarction (AMI) is proving to be a promising approach to repair the injured myocardia. The time window for stem cell transplantation is crucial yet difficult to determine since it produces different therapeutic effects at different times after myocardial infarction. Stromal cell-derived factor-1 (SDF- 1) plays a pivotal role in the mobilization, homing, proliferation, and differentiation of transplanted stem cells. Here, by using ultrasound molecular imaging via targeted microbubbles, we determined the dynamic expression of SDF-1 in a swine model of AMI in vivo . Methods: Twenty-four miniswine were randomly selected for the control group and the AMI model group, which underwent ligation of the left anterior descending coronary artery (LAD). The AMI animals were randomly divided into six experimental groups according to the duration of the myocardial infarction. All animals were subjected to ultrasound molecular imaging through injections with targeted microbubbles (T + T group) or nontargeted control microbubbles (T + C group). The values of the myocardial perfusion parameters (A, β, and A × β) were determined using Q-Lab (Philips ultrasound, version 9.0), and the expression level of SDF-1 was analyzed by real-time polymerase chain reaction (RT-PCR). Results: Our results showed that the expression of SDF-1 gradually increased and peaked at 1 week after AMI. The trend is well reflected by ultrasound molecular imaging in the myocardial perfusion parameters. The A, β, and A × β values correlated with SDF-1 in the T + T group ( r = 0.887, 0.892, and 0.942; P < 0.05). Regression equations were established for the relationships of the A, β, and A × β values ( X ) with SDF-1 ( Y ): Y = 0.699 X − 0.6048, Y = 0.4698 X + 0.3282, and Y = 0.0945 X + 0.6685, respectively ( R 2 = 0.772, 0.7957, and 0.8871; P < 0.05). Conclusions: Our finding demonstrated that ultrasound molecular imaging could be used to evaluate the expression dynamics of SDF-1 after AMI.

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Enhancement of recombinant adeno-associated virus mediated transgene expression by targeted echo-contrast agent

Cited by 4 publications

References 35 publications

Treatment for insomnia in combat-exposed OEF/OIF/OND Military Veterans: Preliminary randomized controlled trial

Treatment for insomnia in combat-exposed OEF/OIF/OND Military Veterans: Preliminary randomized controlled trial

Ultrasound‑targeted microbubble destruction‑mediated Galectin‑7‑siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats

In Vivo Ultrasound Molecular Imaging of SDF-1 Expression in a Swine Model of Acute Myocardial Infarction

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