Enhancement of protein crystallization with the application of Taylor vortex and Poly(ionic liquid)s

Protein Nucleation and Crystallization Process with Process Analytical Technologies in a Batch Crystallizer

“…However, in the hanging drop experiments with very low supersaturation, there were no needle-shaped crystals observed under similar crystallization conditions. The shear was reported to influence the crystallization process and crystal morphology, , and it was assumed that the shear also played an important role in the nucleation of the metastable needle-shaped crystal due to enhancement of mass transfer.…”

Enhancement and Regulation of Peptide Crystallization with the Application of Taylor Vortex: A Case Study of Vancomycin

“…Based on Sun and our previous work, , the employed CT crystallizer, consisting of a colorimetric tube and glass rods placed coaxial, can achieve a stable hydrodynamic state, as shown in Figure a. The size of the glass rod could be adjusted, and specific parameters are listed in Table .…”

“…34 Peptide molecules expose more hydrophobic structure to the solution by the shear stresses of the Taylor vortex, which accelerated the crystallization processes. 19 In addition, the mechanism that shear-induced molecular alignment and in particular agitationenhanced cluster aggregation are put forward in the literature. 28 The molecules tend to align with the flow direction as the shear rate increases.…”

“…18 Moreover, the Taylor vortex can produce a uniform shear force, which can effectively strengthen the crystallization process and obtain crystalline products with a uniform particle size distribution. 19 It is reported that the Taylor vortex has been applied to a variety of processes in industrial crystallization such as reaction crystallization, drowning-out crystallization, and cooling crystallization. 19 Kim et al 20 demonstrated from both experimental and modeling prospects that Taylor vortex has a better mass transfer effect than random turbulence, thus promoting stable polycrystalline nucleation and phase transition of histidine.…”

Recent progress on strategies for the enhancement of crystal growth process

“…12. Tao et al 56 found that different poly (ionic liquids) can significantly regulate lysozyme crystals. Four kinds of poly (ionic liquid), 1-vinyl-3-ethylimidazole bromide (PIL-I), 1-vinyl-3-butylimidazole bromide (PIL-II), 1-allyl-3-vinylimidazole chloride (PIL-III), and 1-allyl-3-vinylimidazole tetrafluoroborate (PIL-IV) were selected as additives.…”