Enhancement of Protein Adsorption Induced by Surface Roughness

Rechendorff, Kristian; Hovgaard, Mads Bruun; Foss, Morten; Zhdanov, Vladimir P.; Besenbacher, Flemming

doi:10.1021/la0621923

Cited by 510 publications

(425 citation statements)

References 24 publications

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“…[58] In contrast, Rechendorff et al showed that fibrinogen adsorption increases with increasing root-mean-square roughness (from 2.0 to 32.9 nm) beyond the accompanying increase in surface area. [59] Interestingly, on the same surface, the relative increase in albumin was closer to the increase in surface area. According to Schulte et al this incoherent picture can be partially understood by large number of parameters affecting the adsorption process, for example, protein concentration, and by a lack of suitable tools for studying adsorption on rough surfaces.…”

Section: Protein Adsorption Guided By Surface Physicochemical Factorsmentioning

confidence: 91%

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Firkowska‐Boden

Zhang

Jandt

2017

Adv Healthcare Materials

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The initial host response to healthcare materials' surfaces after implantation is the adsorption of proteins from blood and interstitial fluids. This adsorbed protein layer modulates the biological/cellular responses to healthcare materials. This stresses the significance of the surface protein assembly for the biocompatibility and functionality of biomaterials and necessitates a profound fundamental understanding of the capability to control protein–surface interactions. This review, therefore, addresses this by systematically analyzing and discussing strategies to control protein adsorption on polymeric healthcare materials through the introduction of specific surface nanostructures. Relevant proteins, healthcare materials' surface properties, clinical applications of polymer healthcare materials, fabrication methods for nanostructured polymer surfaces, amorphous, semicrystalline and block copolymers are considered with a special emphasis on the topographical control of protein adsorption. The review shows that nanostructured polymer surfaces are powerful tools to control the amount, orientation, and order of adsorbed protein layers. It also shows that the understanding of the biological responses to such ordered protein adsorption is still in its infancy, yet it has immense potential for future healthcare materials. The review, which is—as far as it is known—the first one discussing protein adsorption on nanostructured polymer surfaces, concludes with highlighting important current research questions.

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Section: Protein Adsorption Guided By Surface Physicochemical Factorsmentioning

confidence: 91%

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Firkowska‐Boden

Zhang

Jandt

2017

Adv Healthcare Materials

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“…This observation can be attributed to the topology of the thin films [26], where the extent of enzymes on the thin films are lower than SIFs [27]. This is because proteins are known to adsorb more on roughened surfaces [28]. It is also important to note that plasmonic thin films thicker than 2 nm did not result in the enhancement of chemiluminescent emission (data not shown), which can be attributed to other coupling effects observed for semi-continuous and continuous plasmonic thin films [29,30,31,32].…”

Section: Resultsmentioning

confidence: 99%

Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications

et al. 2015

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We report the enhancement of chemiluminescence response of horseradish peroxidase (HRP) in bioassays by plasmonic surfaces, which are comprised of (i) silver island films (SIFs) and (ii) metal thin films (silver, gold, copper, and nickel, 1 nm thick) deposited onto glass slides. A model bioassay, based on the interactions of avidin-modified HRP with a monolayer of biotinylated poly(ethylene-glycol)-amine, was employed to evaluate the ability of plasmonic surfaces to enhance chemiluminescence response of HRP. Chemiluminescence response of HRP in model bioassays were increased up to ~3.7-fold as compared to the control samples (i.e. glass slides without plasmonic nanoparticles), where the largest enhancement of the chemiluminescence response was observed on SIFs with high loading. These findings allowed us to demonstrate the use of SIFs (high loading) for the detection of a biologically relevant target protein (glial fibrillary acidic protein or GFAP), where the chemiluminescence response of the standard bioassay for GFAP was enhanced up to ~50% as compared to bioassay on glass slides.

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“…Next-generation implantable VAD mechanical seals must possess low and steady friction characteristics, a high load-carrying capacity and low leakage of sealed blood. With regard to frictional properties, it has been shown that the creation of a smooth [6] and hydrophilic [7] sealing surface, created by pre-sliding of a polished mechanical seal in water, markedly decreases the friction coefficient under blood sealing conditions [8] through control of protein adsorption on the sealing surface of a VAD mechanical seal.…”

Section: Introductionmentioning

confidence: 99%

Influence of Surface Texture on Friction Properties of Mechanical Seals for Blood -Design Concept of Sealing Surface of Mechanical Seal for Ventricular Assist Device-

Kanda

Sato

Kinoshita³

et al. 2016

Tribology Online

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Low, stable friction, a higher load-carrying capacity, and lower leakage of sealed blood are required for mechanical seals in implantable ventricular assist devices. One solution is to apply surface texture to the mechanical seal surface, consisting of self-mated silicon carbide. However, the effect of surface texture on the frictional properties of mechanical seals under blood sealing conditions has not yet been studied. Therefore, this study aimed to clarify the effect of surface texture on fundamental frictional properties of mechanical seals and to propose design concepts for mechanical seals in ventricular assist devices. The results show that surface texture increases the critical load of mechanical seals, although it also causes periodic peaks in friction. Further, it was found that surface texture induces the formation of denatured protein aggregates on the sealing surface, also inducing periodic surface friction peaks. The frictional properties of the mechanical seals were stabilized by creating small, dispersed concave features with wet blast fabrication, followed by coating with diamond-like carbon. Lower and more stable frictional properties were thus achieved, while simultaneously ensuring a higher critical load.

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Enhancement of Protein Adsorption Induced by Surface Roughness

Cited by 510 publications

References 24 publications

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications

Influence of Surface Texture on Friction Properties of Mechanical Seals for Blood -Design Concept of Sealing Surface of Mechanical Seal for Ventricular Assist Device-

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