Enhancement of Protease Production of Some Bacillus spp. Isolated from Various Regions in Jeddah City

Soliman, Effat A. M.; El-Hamshary, O. I. M.; Batayyib, Reem S. M.

doi:10.20546/ijcmas.2016.505.063

Cited by 1 publication

(1 citation statement)

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“…Enhancement of protease production of some Bacillus spp. by physical and chemical mutagenesis resulted in an increase in productivity by 2-3-fold than the parental strains (Soliman et al, 2016). Combinational exposure of wild strain to UV and EMS gave a better mutant with higher fibrinolytic enzyme activity than using either method (Gopinath et al, 2020).…”

Section: Improvement Of Strain By Etbr or Ems Or Both Mutagenesismentioning

confidence: 98%

Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. Isolated from Vietnamese Traditional Fermented Soybean (Tuong ban) using Ultraviolet Irradiation and Chemical Mutation

Thanh¹,

Hang²,

Anh³

et al. 2022

Int.J.Curr.Microbiol.App.Sci

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Forty-eight out of sixty-eight bacterial strains isolated from traditional fermented soybean samples (Tuong Ban) showed moderate to high fibrinolytic enzyme activity. Bacillus sp. HY6 had the highest fibrinolytic enzyme activity of 80.4 ± 1.2 FU/mL. The strain was selected for improving fibrinolytic activity via UV irradiation and chemically induced mutagenesis using Ethidium Bromide (EtBr) and Ethyl Methane Sulfonate (EMS). After UV-irradiation, the HY6-derived mutant, designated as strain U5_90.5, exhibited the highest fibrinolytic enzyme activity of 127 ± 5 FU/mL, about 1.6 times higher than that of the wild type HY6 strain. Strain U5_90.5 after UV mutation was subsequently subject to chemically induced mutation by EtBr and EMS. This double mutation resulted in strain ES4 which has the highest fibrinolytic enzyme activity of 404 ± 4 FU/mL. The enzyme production capability of the strain was stable after 10 generations which renders the strain a potential fibrinolytic enzyme producing strain for commercial applications. This result indicated that the Bacillus sp. ES4 could be a potential commercialized fibrinolytic enzyme producing bacterium.

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Section: Improvement Of Strain By Etbr or Ems Or Both Mutagenesismentioning

confidence: 98%