Enhancement of preimplantation mouse embryo development with optimized in vitro culture dish via stabilization of medium osmolarity
Hyejin Yoon,
Jongwoo Lee,
Inyoung Kang
et al.
Abstract:Objective: We evaluated the efficacy of the newly developed optimized <i>in vitro</i> culture (OIVC) dish for cultivating preimplantation mouse embryos. This dish minimizes the need for mineral oil and incorporates microwells, providing a stable culture environment and enabling independent monitoring of individual embryos.Methods: Mouse pronuclear (PN) zygotes and two-cell-stage embryos were collected at 18 and 46 hours after human chorionic gonadotropin injection, respectively. These were cultured… Show more
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