Enhancement of Populus alba tolerance to Venturia tremulae upon inoculation with endophytes showing in vitro biocontrol potential

Martínez‐Arias, Clara; Macaya‐Sanz, David; Witzell, Johanna; Martín, Juan A.

doi:10.1007/s10658-018-01618-6

Cited by 20 publications

(13 citation statements)

References 45 publications

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“…In Martínez-Arias et al [266], the successful pretreatment of Populus alba L. seedlings with a spore suspension from endophytes isolated from healthy P. alba trees was shown to significantly strengthen the health status and vitality of the trees towards Venturia tremulae Aderh., i.e., the cause of shoot dieback in Populus. The P. alba endophyte community showed in vitro antagonistic activity towards the pathogen and increased the host's tolerance.…”

Section: Prospects For Translational Applications Of the Knowledge Fomentioning

confidence: 99%

“…The reduction in disease severity indicates the successful antagonistic effect of the endophyte towards the pathogen [190]. The mechanism behind the antagonism could be the endophytes' production of inhibitory secondary metabolites [66,68,252,266], or they may outcompete pathogens for their habitat and nutrients, as demonstrated in several studies [52,66,68,267,268]. Blumenstein et al [66] reported that endophytes have strong potential as biocontrol agents, as their capability for outcompeting plant pathogens might be rather stable and effective.…”

Section: Prospects For Translational Applications Of the Knowledge Fomentioning

confidence: 99%

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Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Terhonen

Blumenstein

Kovalchuk

et al. 2019

Forests

164

113

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Terrestrial plants including forest trees are generally known to live in close association with microbial organisms. The inherent features of this close association can be commensalism, parasitism or mutualism. The term “microbiota” has been used to describe this ecological community of plant-associated pathogenic, mutualistic, endophytic and commensal microorganisms. Many of these microbiota inhabiting forest trees could have a potential impact on the health of, and disease progression in, forest biomes. Comparatively, studies on forest tree microbiomes and their roles in mutualism and disease lag far behind parallel work on crop and human microbiome projects. Very recently, our understanding of plant and tree microbiomes has been enriched due to novel technological advances using metabarcoding, metagenomics, metatranscriptomics and metaproteomics approaches. In addition, the availability of massive DNA databases (e.g., NCBI (USA), EMBL (Europe), DDBJ (Japan), UNITE (Estonia)) as well as powerful computational and bioinformatics tools has helped to facilitate data mining by researchers across diverse disciplines. Available data demonstrate that plant phyllosphere bacterial communities are dominated by members of only a few phyla (Proteobacteria, Actinobacteria, Bacteroidetes). In bulk forest soil, the dominant fungal group is Basidiomycota, whereas Ascomycota is the most prevalent group within plant tissues. The current challenge, however, is how to harness and link the acquired knowledge on microbiomes for translational forest management. Among tree-associated microorganisms, endophytic fungal biota are attracting a lot of attention for their beneficial health- and growth-promoting effects, and were preferentially discussed in this review.

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Section: Prospects For Translational Applications Of the Knowledge Fomentioning

confidence: 99%

Section: Prospects For Translational Applications Of the Knowledge Fomentioning

confidence: 99%

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Terhonen

Blumenstein

Kovalchuk

et al. 2019

Forests

164

113

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“…They thrive in the plant rhizosphere in all natural and managed ecosystems but can also penetrate the roots and establish as endophytes (Compant et al 2005b). In both niches, these microorganisms can improve plant nutrition, increase tolerance to abiotic stresses and enhance protection against phytopathogens (Bonfante and Anca 2009;Martínez-Arias et al 2019). Some bacteria are capable of boosting colonization and establishment of endophytic and mycorrhizal fungi, and thus they have been regarded as mycorrhizal helper bacteria (Garbaye 1994;Frey-Klett et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Interaction between endophytic Proteobacteria strains and Serendipita indica enhances biocontrol activity against fungal pathogens

et al. 2020

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Aims Plants host communities of fungal and bacterial endophytes, establishing a complex network of multipartite interactions, but the mechanisms whereby they interact are poorly understood. Some fungi, such as the beneficial mycorrhiza-like fungus Serendipita (=Piriformospora) indica, can be helped by bacteria for establishment, survival and colonization. Although this fungus harbors a Rhizobium as an endofungal bacterium, we hypothesized that other bacteria might also establish associations with the fungus and combining S. indica with bacteria might enhance plant growth and health. Methods The interactions among S. indica and four e n d o p h y t i c P r o t e o b a c t e r i a b e l o n g i n g t o Methylobacterium, Tardiphaga, Rhodanobacter and Trinickia spp. were characterized in vitro and for their effect on tomato growth and biocontrol of Fusarium oxysporum and Rhizoctonia solani. Possible mechanisms behind these interactions were described based on genome and microscopic analyses, using fungal and bacterial strains tagged with fluorescent markers. Results All bacteria stimulated S. indica growth in vitro. Moreover, several of the bacteria stimulated growth of tomato plants, but co-inoculations with S. indica and bacteria did not perform better than single inoculations. Contrarily, combinations of S. indica and bacteria significantly reduced disease progression of fungal pathogens. These microbes seem to cooperate in the process of root colonization for instance by increasing fungal sporulation and hyphae expansion, showing multipartite interaction between microbes and plants. Interestingly, the strain of Trinickia internally colonizes spores of S. indica as an endofungal bacterium during in vitro-co-culturing, suggesting further that the fungus might acquire formerly unrecognized genera of bacteria and genome analysis of the bacteria revealed many genes potentially involved in fungal and plant growth stimulation, biocontrol and root colonization, highlighting putative mechanisms of plantfungal-bacterial interaction. Conclusions Our study represents an important step towards unraveling the complex interactions among plants, S. indica, endophytic bacteria and fungal pathogens, and indicates that adding bacteria to fungal inoculum could have a remarkable impact on the plantS. indica symbiosis.

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“…Five hundred milligrams of the mycelium were harvested and ground with a pellet pestle. DNA extraction was carried out as described by Martínez-Arias et al (2019). After DNA extraction, the two Internal Transcribed Spacers (ITS) of ribosomal DNA and the large subunit of the rRNA (LSU) were amplified through Polymerase Chain Reaction (PCR) for each fungal strain.…”

Section: Fungal Materialsmentioning

confidence: 99%

“…In recent decades, interest in the study of fungal endophytes and their use as biological control agents has increased (Arnold et al, 2003;Mejía et al, 2008;Fakhro et al, 2010;Martínez-Álvarez et al, 2016;Martínez-Arias et al, 2019;Quiring et al, 2019;Rabiey et al, 2019;Halecker et al, 2020). In forest trees, a successful example of biological control with fungal endophytes is the case of Phialocephala scopiformis, a rugulosin-producing endophyte with the ability to reduce the incidence of the Picea glauca budworm (Miller et al, 2008;Quiring et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Endophyte inoculation enhancesUlmus minorresistance to Dutch elm disease

Martínez‐Arias

Sobrino-Plata

Ormeño-Moncalvillo

et al. 2020

Preprint

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Certain fungal endophytes are known to improve plant resistance to biotic stresses in forest trees. In this study, three stem fungal endophytes belonging to classes Cystobasidiomycetes, Eurotiomycetes and Dothideomycetes were selected among 210 isolates for their potential as enhancers of Ulmus minor resistance to Ophiostoma novo-ulmi. We evaluated phenotypic traits of these endophytes that could be beneficial for O. novo-ulmi inhibition in the host plant. Under in vitro conditions, the Dothideomycetous isolate YCB36 strongly inhibited O. novo-ulmi growth, released antipathogen VOCs, chitinases and siderophores, and overlapped with the pathogen in nutrient utilization patterns. These functional traits could explain the 40% reduction in leaf wilting due to O. novo-ulmi in elm trees pre-inoculated with this endophyte. Ulmus minor trees responded to endophyte inoculation with increased stomatal conductance and higher amounts of flavonoids and total phenolic compounds in xylem tissues, suggesting induction of defence metabolism.

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Enhancement of Populus alba tolerance to Venturia tremulae upon inoculation with endophytes showing in vitro biocontrol potential

Cited by 20 publications

References 45 publications

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Interaction between endophytic Proteobacteria strains and Serendipita indica enhances biocontrol activity against fungal pathogens

Endophyte inoculation enhancesUlmus minorresistance to Dutch elm disease

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