Enhancement of Phytase Production from a New Probiotic Strain Bacillus subtilis P6

Trivedi, Shraddha; Sharma, Anjana; Jain, P. C.

doi:10.20546/ijcmas.2017.606.328

Cited by 4 publications

(5 citation statements)

References 13 publications

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“…Another researcher [24] found that the maximum phytase production of Enterobacter and Serratia species occurred at 48 h of growth. The maximum phytase production has been detected after 44 h of incubation from Bacillus subtilis P6 [25]. It was obtained that maximum production of phytase at 48 h was shown by Achromobacter sp [26].…”

Section: Optimization Of Growth Media 331 the Influence Of Incubation Time Of Phytase Productionmentioning

confidence: 89%

“…Optimization of growth condition for maximum phytase production was carried out by inoculating the selected bacterial isolates in the phytase production medium for 72 h under different conditions: temperatures (25,30,35,40,45,50) °C; pH (3.5, 4.5, 5.5, 6.5, 7.5, and 8,5); carbon sources such as sucrose, maltose, lactose, dextrose and glucose (control); nitrogen sources including tryptone, peptone, yeast extract and beef extract, ammonium chloride and ammonium sulfate (control); P sources such as Ca3PO4, KH2PO4, NaH2PO4 and concentrations of NaCl (1, 2,3, 4, 5, 6%)…”

Section: Optimization On Phytase Productionmentioning

confidence: 99%

“…EBD and Enterobacter cloacae showed the best phytase production with the addition of lactose as a C source [18,42]. Furthermore, [25] noted that glucose resulted in optimal phytase production (12.23 IU/mL) from Bacillus subtilis P6.…”

Section: The Influence Of C Sources On Phytase Productionmentioning

confidence: 99%

“…It was reported that the positive result of phytase production of Pseudomonas AP-MSU 2 was achieved with the use of sodium dihydrogen orthophosphate, whereas disodium hydrogen ortho phosphate, calcium hydrogen orthophosphate and bismuth phosphate had a negative effect [43]. Furthermore, [25] noted that phytase production from Bacillus subtilis P6 could be stimulated in media enriched with K2HPO4.…”

Section: The Influence Of P Sources On Phytase Productionmentioning

confidence: 99%

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Phytase activity of phytase-producing bacteria isolated from mangrove sediment

Suliasih

Widawati

Ikhwani

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Through the role of phytase, some soil bacteria can mineralize insoluble organic P such as phytate (myoinositol hexakisphosphate). Phytase is a secreted enzyme possessing the ability to hydrolyze phytate into phosphate esters and inorganic P for plants absorption. This study aimed to isolate phytase-producing bacteria from mangrove sediment and examine the influence of nutrients (source of carbon, nitrogen, phosphorus) and physical conditions (temperature, pH, NaCl tolerance) on maximum phytase production. The presence of phytase activity was determined by examining the individual colonies for the formation of a clear zone. Furthermore, the isolates were screened qualitatively and quantitatively using solid and liquid phytase screening medium (PSM) containing sodium Phytate as substrates. The result showed that a total of 48 isolates have the potential to produce phytase with a production range of 1.11 - 14.83U/mL. The isolate F15 as Bacillus altitudinis was found to produce the highest phytase after 72 hours of incubation, was selected for further analysis. This strain resulted in optimal phytase levels at 35°C and a pH of 6.5 in physical parameters, tolerated 5% NaCl in the presence of lactose and tryptone, which served as carbon and nitrogen sources, respectively.

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Section: Optimization Of Growth Media 331 the Influence Of Incubation Time Of Phytase Productionmentioning

confidence: 89%

Section: Optimization On Phytase Productionmentioning

confidence: 99%

Section: The Influence Of C Sources On Phytase Productionmentioning

confidence: 99%

Section: The Influence Of P Sources On Phytase Productionmentioning

confidence: 99%

See 2 more Smart Citations

Phytase activity of phytase-producing bacteria isolated from mangrove sediment

Suliasih

Widawati

Ikhwani

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…Our group is working on bacterial enzymes, and previously we isolated different enzymes from indigenous bacterial strains (Bala et al 2020;Husain et al, 2016aHusain et al, , 2016b. We also isolated indigenous probiotic B. subtilis P6 from poultry soil with excellent phytase-producing ability (Sharma & Trivedi, 2015) and then optimized various process parameters for the maximum yield of phytase (Trivedi et al, 2017). In the present investigation, we purified and characterized phytase from B. subtilis P6 and evaluated its probiotic properties using various biochemical and cellular assays.…”

mentioning

confidence: 98%

Purification and characterization of phytase from Bacillus subtilis P6: Evaluation for probiotic potential for possible application in animal feed

2021

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The antinutritional property of phytate is the main problem associated with food animals, but phytase-producing probiotic strain could be the best alternative for resolving this problem. In the present study, phytase from probiotic strain Bacillus subtilis P6 was purified to homogeneity, which showed a relative molecular mass of 40 kDa on native and SDS-PAGE. The purification fold of purified phytase was 86.99, with a specific activity of 104.39 U/mg protein. The purified enzyme showed optimum activity at 40 • C and pH 6.0. Semi-metal ions, such as K + and Ca 2+ , slightly enhance the enzyme activity while Cu 2+ , Co 2+ , and Zn 2+ prominently inhibited phytase activity. Purified phytase showed the highest substrate specificity toward its natural substrate phytate and did not hydrolyze other substrates having a phosphate bond. The purified enzyme exhibited strong proteolytic tolerance against trypsin and pepsin. Further, we evaluated the probiotic potential of B. subtilis and noted that it showed excellent resistance toward high acidic and bile concentrations. Subsequently, it revealed exceptional antimicrobial activity, good hydrophobicity, autoaggregation ability, nonhemolytic, and nonlecithinase activity. Additionally, B. subtilis P6 displayed susceptibility against major antibiotics. Owing to the excellent biochemical properties of phytase with notable probiotic characteristics, B. subtilis P6 may offer the possibility of being a promising candidate for the application in animal feed and food supplements. However, the in vivo use of strain secreting phytase needs to be studied in animals for its intended benefits

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