Enhancement of Oxidative Stress Tolerance in Transgenic Tobacco Plants Overproducing Fe-Superoxide Dismutase in Chloroplasts

Camp, Wim Van; Capiau, K.; Montagu, Marc Van; Inzé, Dirk; Slooten, Luit

doi:10.1104/pp.112.4.1703

Cited by 318 publications

(139 citation statements)

References 44 publications

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“…Nevertheless, in transgenic tobacco plants, in which an Fe-SOD from Arabidopsis thaliana is overexpressed, induction of cytosolic and chloroplastic CuZn-SODs was suppressed. This suggests that the transgenic Fe-SOD interfered with a pathway signal leading to the induction of CuZn-SODs isozymes, probably because the concentration of O # n − in the chloroplasts is lowered by transgenic Fe-SOD (Van Camp et al, 1996). In our study, the induction of mitochondrial and peroxisomal Mn-SOD and of CuZn-SOD I (Table 3), mainly localized in the cytosol, was brought about by NaCl stress.…”

Section:   mentioning

confidence: 63%

“…These differences in the response to NaCl stress seem to indicate a differential regulation of both SOD isozymes and could also suggest a specific function for each of the enzymes in protecting chloroplasts. The increase in Fe-SOD activity as well as the induction of cytosolic and chloroplastic CuZn-SODs by salt stress have recently been reported in tobacco plants treated with 60 mol m −$ NaCl (Van Camp et al, 1996). Nevertheless, in transgenic tobacco plants, in which an Fe-SOD from Arabidopsis thaliana is overexpressed, induction of cytosolic and chloroplastic CuZn-SODs was suppressed.…”

Section:   mentioning

confidence: 99%

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Response of antioxidant systems and leaf water relations to NaCl stress in pea plants

et al. 1999

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A pea (Pisum sativum cv. Puget) cultivar was grown on a medium containing different NaCl concentrations (0-160 mol m −$ ) in order to study the effects of salt stress on leaf water relations and on the activity of antioxidant enzymes. NaCl stress caused a rapid decline in chlorophyll content. Both leaf water (ψ l ) and osmotic potentials (ψ s ) decreased progressively with the severity of the stress (from 90-160 mol m −$ NaCl) whereas leaf turgor pressure (ψ p ) increased in treated plants. Pea leaves contained an iron-containing superoxide dismutase (Fe-SOD) isozyme in chloroplasts alongside a copper-zinc-containing (CuZn-SOD) form (CuZn-SOD II). The lowest NaCl concentration (70 mol m −$ ) had no effect on the activity of these antioxidant enzymes while higher concentrations (110-130 mol m −$ ) enhanced the activity of cytosolic CuZn-SOD I and chloroplastic CuZn-SOD II as well as that of mitochondrial and\or peroxisomal manganese-containing superoxide dismutase (Mn-SOD). These inductions were matched by increases in the activity of ascorbate peroxidase (APX) and monodehydroascorbate reductase (MDHAR). The increased activities coincided with decreased stomatal conductance and were unaffected by the severity of stress except in the case of CuZn-SOD II which fell to control values under the highest stress conditions (140-160 mol m −$ NaCl), when a concomitant increase in chloroplastic Fe-SOD activity was observed. Glutathione reductase (GR) and dehydroascorbate reductase (DHAR) activities were only induced under severe NaCl stress (130-160 mol m −$ ) and were accompanied by losses in the ascorbate and glutathione pools, lower ASC\DHA and GSH\GSSG ratios and increases in GSSG. Electron microscopy showed that the thylakoidal structure of the chloroplasts became disorganized and their starch content decreased in plants treated with 160 mol m −$ NaCl. Overall, the results suggest that salt stress is accompanied by oxidative stress, perhaps at the cell compartment level. The capacity of Puget cultivar to ensure cell turgor and to enhance the activity of enzymes involved in the defence against oxidative stress seems to be important in determining adaptation to moderate NaCl stress conditions. In plants exposed to severe NaCl stress (130-160 mol m −$ ) it seems that such resistance to oxidative stress is overcome, which might contribute to the deleterious effects of salt and significant growth reduction in these conditions.

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Section:   mentioning

confidence: 63%

Section:   mentioning

confidence: 99%

Response of antioxidant systems and leaf water relations to NaCl stress in pea plants

et al. 1999

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“…Aliquots of frozen fresh shoot material (0.5 g) were ground to a fine powder with liquid nitrogen and homogenized in 1.5 mL of a cold solution containing isoforms were appeared with brown bands after 10 to 20 min at 4°C (Vallejos, 1983). This experiment was repeated three times with similar results.…”

Section: Protein Extractionmentioning

confidence: 84%

“…Several genes encoding for plant antioxidant enzymes have been cloned, characterized, and used in the construction of transgenic lines. Transgenic plants overexpressing SOD (Bowler et al, 1991;Van Camp et al, 1996;Breusegem et al, 1999;Alscher et al, 2002;Feki et al, 2016), APX (Wang et al, 1999), GR (Foyer et al, 1995), GPX (Roxas et al, 1997(Roxas et al, , 2000 and CAT (Feki et al, 2015) showed tolerance to various abiotic stresses.…”

Section: Introductionmentioning

confidence: 99%

Comparison of an antioxidant system in tolerant and susceptible wheat seedlings in response to salt stress

Feki

Tounsi

Brini

2018

Span. j. agric. res.

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It has been demonstrated previously that the physiological and molecular analysis of seedlings of the tolerant (Om Rabia3) and susceptible (Mahmoudi) Tunisian wheat genotypes were different at short and long-term response to salinity. In this study, we examined the antioxidant defence system in seedlings of these two cultivars at short-term response to different NaCl concentrations. The findings showed that high salinity tolerance of cv. Om Rabia3, as manifested by lower decrease in its dry biomass, was associated with lower malondialdehyde and hydrogen peroxide contents, lower accumulation of the superoxide (O 2 -) in the roots and the shoots, and also lower decrease in ascorbate content than those in cv. Mahmoudi. Moreover, the expression of some genes coding for antioxidant enzymes such as the catalase, the superoxide dismutase and the peroxidase were enhanced by NaCl stress especially in the salt-tolerant cultivar. In parallel, their activities were increased in response to the same condition of stress and especially in the cv. Om Rabia3. Taken together, these data suggested that the capacity to limit oxidative damage is important for NaCl tolerance of durum wheat.

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“…An NaCl-tolerant cultivar of pea, compared with a sensitive cultivar, had enhanced chloroplastic and cytosolic CuZn-SOD activity when treated with NaCl (Hernandez et al, 1995). In transgenic tobacco plants, salt stress was associated with overexpression of cytosolic and chloroplastic CuZn-SOD enzymes (Van Camp et al, 1996). Drought stress represents another oxidative environmental factor causing enhanced production of O # d − and H # O # , particularly in chloroplasts (Price & Hendry, 1989Smirnoff, 1993 ;Bohnert et al, 1995).…”

Section:       mentioning

confidence: 99%

Tansley Review No. 111

2000

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Zinc deficiency is one of the most widespread micronutrient deficiencies in plants and causes severe reductions in crop production. There are a number of physiological impairments in Zn-deficient cells causing inhibition of the growth, differentiation and development of plants. Increasing evidence indicates that oxidative damage to critical cell compounds resulting from attack by reactive O # species (ROS) is the basis of disturbances in plant growth caused by Zn deficiency. Zinc interferes with membrane-bound NADPH oxidase producing ROS. In Zn-deficient plants the iron concentration increases, which potentiates the production of free radicals. The Zn nutritional status of plants influences photooxidative damage to chloroplasts, catalysed by ROS. Zinc-deficient leaves are highly light-sensitive, rapidly becoming chlorotic and necrotic when exposed to high light intensity. Zinc plays critical roles in the defence system of cells against ROS, and thus represents an excellent protective agent against the oxidation of several vital cell components such as membrane lipids and proteins, chlorophyll, SH-containing enzymes and DNA. The cysteine, histidine and glutamate or aspartate residues represent the most critical Znbinding sites in enzymes, DNA-binding proteins (Zn-finger proteins) and membrane proteins. In addition, animal studies have shown that Zn is involved in inhibition of apoptosis (programmed cell death) which is preceded by DNA and membrane damage through reactions with ROS.

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Enhancement of Oxidative Stress Tolerance in Transgenic Tobacco Plants Overproducing Fe-Superoxide Dismutase in Chloroplasts

Cited by 318 publications

References 44 publications

Response of antioxidant systems and leaf water relations to NaCl stress in pea plants

Response of antioxidant systems and leaf water relations to NaCl stress in pea plants

Comparison of an antioxidant system in tolerant and susceptible wheat seedlings in response to salt stress

Tansley Review No. 111

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