Enhancement of ovarian cancer chemotherapy by delivery of multidrug‐resistance gene small interfering <scp>RNA</scp> using tumor targeting <i><scp>S</scp>almonella</i>

Deng, Jiaqi; Guo, Yi; Jiang, Zhongmin; Yang, Min; Li, Huaifang; Wang, Jianjun

doi:10.1111/jog.12598

Cited by 23 publications

(15 citation statements)

References 15 publications

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“…Genetically attenuated bacteria such as attenuated Salmonella offers promise as an anticancer vector and has been widely used as a tool to deliver plasmids that express siRNA in vivo 16–21 . Our previous studies have demonstrated that a combination of low-dose DDP with gene therapy expressing tumor protein p53 (p53) and mouse double minute 2 homolog (MDM2) siRNA, delivered by attenuated Salmonella enterica serovar Typhi vaccine strain Ty21a ( Salmonella Typhi Ty21a ), synergistically inhibited ovarian cancer and PCa growth without damaging normal tissues 22–25 .…”

Section: Introductionmentioning

confidence: 99%

Knockdown of HIF-1α by siRNA-expressing plasmid delivered by attenuated Salmonella enhances the antitumor effects of cisplatin on prostate cancer

Zeng

et al. 2017

Sci Rep

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Resistance to cisplatin (DDP) and dose-related toxicity remain two important obstacles in the treatment of prostate cancer (PCa) patients with DDP-based chemotherapy. We have investigated whether the knockdown of hypoxia-inducible factor-1 alpha (HIF-1α) by siRNA could enhance the antitumor activity of DDP, and aimed to determine the underlying mechanisms. Intravenous injection of attenuated Salmonella carrying a HIF-1α siRNA-expressing plasmid was used to knockdown HIF-1α in a PC-3 xenograft model. The in vitro and in vivo effects of HIF-1α siRNA treatment and/or DPP on PCa cell proliferation, apoptosis, glycolysis, and production of reactive oxygen species (ROS) were assessed by examining molecular markers specific to each process. The results demonstrated that the treatment of tumor-bearing mice with attenuated Salmonella carrying the HIF-1α siRNA plasmid greatly enhanced the antitumor effects of low-dose DDP. Further mechanistic studies demonstrated that knockdown of HIF-1α improved the response of PCa cells to DDP by redirecting aerobic glycolysis toward mitochondrial oxidative phosphorylation, leading to cell death through overproduction of ROS. Our findings indicate that DDP-based chemotherapy combined with targeting the HIF-1α-regulated cancer metabolism pathway might be an ideal strategy to treat PCa.

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Section: Introductionmentioning

confidence: 99%

Knockdown of HIF-1α by siRNA-expressing plasmid delivered by attenuated Salmonella enhances the antitumor effects of cisplatin on prostate cancer

Zeng

et al. 2017

Sci Rep

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“…P-gp is a highly conserved membrane glycoprotein encoded by the MDR1 gene. In one study, Deng et al, assessed the effects of attenuated Salmonella typhi acting as a tumor-targeted vector to deliver small interfering RNA (siRNA) against the multidrug-resistance gene (MDR1) ( 45 ). This was tested in the cisplatin-resistant ovarian cancer cell line SKOV-3/DDP, to increase cisplatin sensitivity.…”

Section: Salmonella Bacteria As Delivery Systems For Anti-tumentioning

confidence: 99%

“…The tumor growth in mice was slower when treated with DDP. Attenuated Salmonella bacteria could be used for delivery of RNA interference as a component of cancer treatment ( 45 ).…”

Section: Salmonella Bacteria As Delivery Systems For Anti-tumentioning

confidence: 99%

Use of Salmonella Bacteria in Cancer Therapy: Direct, Drug Delivery and Combination Approaches

et al. 2021

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Over the years, conventional cancer treatments, such as chemotherapy with only a limited specificity for tumors, have undergone significant improvement. Moreover, newer therapies such as immunotherapy have undergone a revolution to stimulate the innate as well as adaptive immune responses against the tumor. However, it has been found that tumors can be selectively colonized by certain bacteria, where they can proliferate, and exert direct oncolytic effects as well as stimulating the immune system. Bacterial-mediated cancer therapy (BMCT) is now one example of a hot topic in the antitumor field. Salmonella typhimurium is a Gram-negative species that generally causes self-limiting gastroenteritis in humans. This species has been designed and engineered in order to be used in cancer-targeted therapeutics. S. typhimurium can be used in combination with other treatments such as chemotherapy or radiotherapy for synergistic modification of the tumor microenvironment. Considerable benefits have been shown by using engineered attenuated strains for the diagnosis and treatment of tumors. Some of these treatment approaches have received FDA approval for early-phase clinical trials. This review summarizes the use of Salmonella bacteria for cancer therapy, which could pave the way towards routine clinical application. The benefits of this therapy include an automatic self-targeting ability, and the possibility of genetic manipulation to produce newly engineered attenuated strains. Nevertheless, Salmonella-mediated anticancer therapy has not yet been clinically established, and requires more research before its use in cancer treatment.

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“…The application of siRNAs in the attempt to interfere with tumors has been primarily at the cell culture level rather than the whole organism [108] . Nevertheless, this approach does open up a new way to modify tumor cells and a broad variety of delivery systems have been employed in studies on OC [126][127][128][129][130][131][132][133] . Table 2 gives an overview on the deregulated circulating miRNAs detected in plasma, serum, ascites, urine and exosomes from OC patients and their function in EMT, migration, invasion and metastasis.…”

Section: Sirnas and Mrnasmentioning

confidence: 99%

Circulating non-coding RNAs in recurrent and metastatic ovarian cancer

Schwarzenbach¹,

Gahan²

2019

CDR

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Ovarian cancer has a poor outcome because it is usually detected at advanced tumor stages, and the majority of the patients develop disease relapse as a result of chemotherapy resistance. This most lethal gynecological malignancy metastasizes within the peritoneal fluid or ascites to pelvic and distal organs. In ovarian cancer progression and metastasis, small non-coding RNAs (ncRNAs), including long noncoding RNAs and microRNAs have been recognized as important regulators. Their dysregulation modulates gene expression and cellular signal pathways and can be detected in liquid biopsies. In this review, we provide an overview on circulating plasma and serum ncRNAs participating in tumor cell migration and invasion, and contributing to recurrence and metastasis of ovarian cancer. We will also discuss the development of potential, novel therapies using ncRNAs as target molecules or tumor markers for ovarian cancer.

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Enhancement of ovarian cancer chemotherapy by delivery of multidrug‐resistance gene small interfering RNA using tumor targeting Salmonella

Abstract: Attenuated Salmonella typhi may represent a promising vector for in vivo administration of RNA interference therapy against malignant tumors.

Cited by 23 publications

References 15 publications

Knockdown of HIF-1α by siRNA-expressing plasmid delivered by attenuated Salmonella enhances the antitumor effects of cisplatin on prostate cancer

Knockdown of HIF-1α by siRNA-expressing plasmid delivered by attenuated Salmonella enhances the antitumor effects of cisplatin on prostate cancer

Use of Salmonella Bacteria in Cancer Therapy: Direct, Drug Delivery and Combination Approaches

Circulating non-coding RNAs in recurrent and metastatic ovarian cancer

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