Enhancement of nitric oxide and proteoglycan synthesis due to cyclic tensile strain loaded on chondrocytes attached to fibronectin

Matsukawa, M.; Fukuda, Kanji; Yamasaki, Katsuhide; Yoshida, Kohji; Munakata, Hiroshi; Hamanishi, Chiaki

doi:10.1007/s00011-004-1254-4

Cited by 13 publications

(11 citation statements)

References 33 publications

(42 reference statements)

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“…In particular, Matsukawa et al (2004) observed that on fibronectin coated plates, proteoglycans increased. Whereas on collagen I coated culture plates, proteoglycans were decreased [ 31 , 47 ]. On pronectin coated plates, no changes between unloaded cells and cells under CTS were observed [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…Only one study found an increase in iNOS and NO at 0.05 Hz [ 76 ]. However, higher frequencies (0.17 and 0.5 Hz) up-regulated especially COX-2 and with increasing loading duration also iNOS, NO and PGE 2 [ 26 , 28 , 36 , 37 , 47 ]. Matsukawa et al (2004) reported that CTS stimulated iNOS mRNA only on fibronectin coated culture plates, but not on collagen coating.…”

Section: Resultsmentioning

confidence: 99%

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Effects of Cyclic Tensile Strain on Chondrocyte Metabolism: A Systematic Review

et al. 2015

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Chondrocytes reorganize the extracellular matrix of articular cartilage in response to externally applied loads. Thereby, different loading characteristics lead to different biological responses. Despite of active research in this area, it is still unclear which parts of the extracellular matrix adapt in what ways, and how specific loading characteristics affect matrix changes. This review focuses on the influence of cyclic tensile strain on chondrocyte metabolism in vitro. It also aimed to identify anabolic or catabolic chondrocyte responses to different loading protocols. The key findings show that loading cells up to 3% strain, 0.17 Hz, and 2 h, resulted in weak or no biological responses. Loading between 3–10% strain, 0.17–0.5 Hz, and 2–12 h led to anabolic responses; and above 10% strain, 0.5 Hz, and 12 h catabolic events predominated. However, this review also discusses that various other factors are involved in the remodeling of the extracellular matrix in response to loading, and that parameters like an inflammatory environment might influence the biological response.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effects of Cyclic Tensile Strain on Chondrocyte Metabolism: A Systematic Review

et al. 2015

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“…This study evaluated one level of chronic load alteration (100%BW) at two select time points (6 and 20 weeks) and unquestionably demonstrates the induction of joint degeneration by altered load. In vitro studies have shown that abnormal mechanical forces can stimulate articular chondrocytes to produce pro-inflammatory mediators [33–36], that promote matrix degradation through a variety of metabolic pathways. The use of the VLD-model will allow elucidation of the mechanisms of in vivo chronic load-induced joint degeneration in future studies which evaluate early changes following shorter durations of altered loading.…”

Section: Discussionmentioning

confidence: 99%

Chronic in vivo load alteration induces degenerative changes in the rat tibiofemoral joint

Roemhildt

Beynnon

Gauthier

et al. 2013

Osteoarthritis and Cartilage

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Objective We investigated the relationship between the magnitude and duration of sustained compressive load alteration and the development of degenerative changes in the rat tibiofemoral joint. Methods A varus loading device was attached to the left hind limb of mature rats to apply increased compression to the medial compartment and decreased compression to the lateral compartment of the tibiofemoral joint of either 0% or 100% body weight for 0, 6 or 20 weeks. Compartment-specific assessment of the tibial plateaus included biomechanical measures (articular cartilage aggregate modulus, permeability and Poisson’s ratio, and subchondral bone modulus) and histological assessments (articular cartilage, calcified cartilage, and subchondral bone thicknesses, degenerative scoring parameters, and articular cartilage cellularity). Results Increased compression in the medial compartment produced significant degenerative changes consistent with the development of osteoarthritis including a progressive decrease in cartilage aggregate modulus (43% and 77% at 6 and 20 weeks), diminished cellularity (38% and 51% at 6 and 20 weeks), and increased histological degeneration. At 20 weeks, medial compartment articular cartilage thickness deceased 30% while subchondral bone thickness increased 32% and subchondral bone modulus increased 99%. Decreased compression in the lateral compartment increased calcified cartilage thickness, diminished region-specific subchondral bone thickness and revealed trends for reduced cellularity and decreased articular cartilage thickness at 20 weeks. Conclusions Altered chronic joint loading produced degenerative changes consistent with those observed clinically with the development of osteoarthritis and may replicate the slow development of non-traumatic osteoarthritis in which mechanical loads play a primary etiological role.

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“…Production of proteoglycans containing acidic glycosaminoglycans as well as production of hyaluronic acid by cells is upregulated by cyclic shearing, tensile and compression strain (Dowthwaite et al, 1999;Lee et al, 2000;Matsukawa et al, 2004;Grad et al, 2005). These substances entrap large quantities of water, which is removed from the proteoglycan aggregates when pressure is applied.…”

Section: Discussionmentioning

confidence: 99%

Renaut bodies in nerves of the trunk of the African elephant, Loxodonta africana

Witter

Egger

Boeck

2007

Journal of Morphology

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Renaut bodies are loosely textured, cell-sparse structures in the subperineurial space of peripheral nerves, frequently found at sites of nerve entrapment. The trunk of the elephant is a mobile, richly innervated organ, which serves for food gathering, object grasping and as a tactile organ. These functions of the trunk lead to distortion and mechanical compression of its nerves, which can therefore be expected to contain numerous Renaut bodies. Samples of the trunk wall of an adult African elephant (Loxodonta africana) were examined histologically using conventional staining methods, immunohistochemistry, and lectin histochemistry. Architecture of nerve plexuses and occurrence of Renaut bodies in the elephant trunk were compared with those in tissues surrounding the nasal vestibule of the pig. Prominent nerve plexuses were found in all layers of the elephant trunk. Almost all (81%) nerve profiles contained Renaut bodies, a basophilic, discrete subperineurial layer resembling cushions around the nerve core. In contrast, Renaut bodies were seen in only 15% of nerve profiles in the porcine nasal vestibule. Within Renaut bodies, fusiform fibroblasts and round, ruff-like cells were placed into a matrix of acidic glycosaminoglycans with delicate collagen and very few reticular fibers. The turgor of this matrix is thought to protect nerves against compression and shearing strain. Renaut bodies are readily stained with alcian blue (pH 2.5) favorably in combination with immunohistochemical markers of nerve fibers. They should be regarded as a physiological response to repeated mechanical insults and are distinct from pathological alterations. alterations.

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Enhancement of nitric oxide and proteoglycan synthesis due to cyclic tensile strain loaded on chondrocytes attached to fibronectin

Abstract: Cyclic tensile strain loaded on the chondrocytes enhanced NO synthesis and this enhanced NO inhibited PG synthesis.

Cited by 13 publications

References 33 publications

Effects of Cyclic Tensile Strain on Chondrocyte Metabolism: A Systematic Review

Effects of Cyclic Tensile Strain on Chondrocyte Metabolism: A Systematic Review

Chronic in vivo load alteration induces degenerative changes in the rat tibiofemoral joint

Renaut bodies in nerves of the trunk of the African elephant, Loxodonta africana

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