Enhancement of neurite adhesion, alignment and elongation on conductive polypyrrole-poly(lactide acid) fibers with cell-derived extracellular matrix

Zhou, Xingxing; Yang, Anneng; Huang, Zhongbing; Yin, Guangfu; Pu, Ximing; Jin, Juan

doi:10.1016/j.colsurfb.2016.10.014

Cited by 83 publications

(70 citation statements)

References 46 publications

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“…Especially for the scaffolds collected on a flat plate (randomly oriented fibers), measuring fiber density or scaffold porosity may provide a better measure of the suitability of the scaffolds for hosting cells than fiber diameter. Previous studies that electrospun conductive particles have obtained similar fiber diameters . As we have found in our previous work, the addition of conductive particles may destabilize the electrospinning process, but it did not prevent a fibrous scaffold from being formed…”

Section: Discussionsupporting

confidence: 74%

Optimizing C2C12 myoblast differentiation using polycaprolactone–polypyrrole copolymer scaffolds

Browe

Freeman

2018

J Biomedical Materials Res

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Advancements in tissue engineering and biomaterial development have the potential to provide a scalable solution to the problem of large-volume skeletal muscle defects. Previous research on the development of scaffolds for skeletal muscle regeneration has focused on strategies for increasing conductivity, which has improved satellite cell attachment and differentiation. However, these strategies usually increase scaffold stiffness, which some studies suggest may be detrimental to myoblast development. In this study, the polymers polypyrrole (PPy) and polycaprolactone (PCL) were synthesized together into a copolymer (PPy-PCL) designed to increase scaffold conductivity without significantly influencing stiffness. Different scaffold groups were fabricated via electrospinning, characterized, and assessed for their suitability for myoblast proliferation and differentiation. The groups included an aligned and random iteration of pure PCL, 10% PPy-PCL, 20% PPy-PCL, and 40% PPy-PCL. Only the 40% PPy-PCL group had a measureable conductivity, and the addition of PPy-PCL had no significant effect on the stiffness of the scaffolds. The PPy-PCL copolymer significantly increased the attachment of C2C12 myoblasts as compared to pure PCL scaffolds, but the concentration of PPy-PCL did not significantly alter cell attachment. In addition, scaffolds with PPy-PCL promoted myoblast differentiation to a greater extent than scaffolds made of PCL as measured by fusion index and number of nuclei per myotube. Aligned scaffolds were superior to random scaffolds in almost all measures. These results suggest that conductivity may not be the key factor in improving skeletal muscle scaffolds. Instead, cell attachment and aligned guidance cues may have a greater impact on myoblast differentiation.

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Section: Discussionsupporting

confidence: 74%

Optimizing C2C12 myoblast differentiation using polycaprolactone–polypyrrole copolymer scaffolds

Browe

Freeman

2018

J Biomedical Materials Res

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“…Due to characteristics of the neural tissue, full regeneration of the central nervous system is still not possible (25,26) and is been sought by different means, including stem cell therapies (27,28), and engineered materials (29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

“…Our results showed that Neuro2a cultured on PBAT/PPy2% and treated with RA in 0.5 % FBS have more and longer neurites when compared to cells grown on PBAT only (Figure 4). The use of PPy combined with other degradable polymers such as PLCL, PLLA, PCL has already shown promising results regarding neuronal viability and neurite extension, especially when neuronal cells are electrically stimulated (30,46,47).…”

Section: Discussionmentioning

confidence: 99%

Polypyrrole increases branching and neurite extension by Neuro2A cells on PBAT ultrathin fibers

Granato

Ribeiro

Marciano

et al. 2017

Preprint

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Electrospinning shows a feasible way to generate hybrid scaffolds from the combination of different materials. This work presented a successful route to prepare ultrathin fibers from hybrid solutions containing a commercial polyester, poly (butylene adipate-co-terephthalate) (PBAT) and a conductive polymer, polypyrrole (PPy). The final material (PBAT/PPy) showed an enhanced potential for neuronal differentiation when compared to neat PBAT. The PPy loading improved branching and neurite extension of Neuro2a cells, which opens a wide range of perspectives where these materials may be applied in regenerative medicine.. CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/241307 doi: bioRxiv preprint first posted online Dec. 30, 2017; 3 ABSTRACTWe present a methodology for production and application of electrospun hybrid materials containing commercial polyester (poly (butylene adipate-coterephthalate; PBAT), and a conductive polymer (polypirrole; PPy) as scaffold for neuronal growth and differentiation. The physical-chemical properties of the scaffolds and optimization of the electrospinning parameters are presented.The electrospun scaffolds are biocompatible and allow proper adhesion and spread of mesenchymal stem cells (MSCs). Fibers produced with PBAT with or without PPy were used as scaffold for Neuro2a mouse neuroblastoma cells adhesion and differentiation. Neuro2a adhered to PBAT and PBAT/PPy2% scaffolds without laminin coating. However, Neuro2a failed to differentiate in PBAT when stimulated by treatment with retinoic acid (RA), but differentiated in PBAT/PPy2% fibers. We hypothesize that PBAT hydrophobicity inhibited proper spreading and further differentiation, and inhibition was overcome by coating the PBAT fibers with laminin. We conclude that fibers produced with the combination of PBAT and PPy can support neuronal differentiation.

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“…A further improvement would be to functionalize the scaffold with biomolecules which can assist the stem cells to differentiate toward a specific nerve (Table ). Laminin has been employed for this purpose as it is found in the ECM of nervous tissue . Koh et al studied the impact of laminin functionalization technique in poly(L‐lactic acid) (PLLA) nanofiber scaffolds.…”

Section: Tissue Engineering Scaffolds For Alzheimer’s and Parkinson'smentioning

confidence: 99%

Tissue engineering scaffolds in the treatment of brain disorders in geriatric patients

et al. 2019

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The world population is ageing at an alarming rate, currently increasing at around 3% per year for people over 60 years. This fast growing demography is largely unproductive and prone to many brain disorders such as Alzheimer's disease, Parkinson's disease, and brain tumors. Currently available treatment modalities are inadequate to stop neural degeneration or to completely eradicate cancer cells. Exogenously engineered scaffolds hold great potential for in vivo brain regeneration and functional restoration.

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Enhancement of neurite adhesion, alignment and elongation on conductive polypyrrole-poly(lactide acid) fibers with cell-derived extracellular matrix

Cited by 83 publications

References 46 publications

Optimizing C2C12 myoblast differentiation using polycaprolactone–polypyrrole copolymer scaffolds

Optimizing C2C12 myoblast differentiation using polycaprolactone–polypyrrole copolymer scaffolds

Polypyrrole increases branching and neurite extension by Neuro2A cells on PBAT ultrathin fibers

Tissue engineering scaffolds in the treatment of brain disorders in geriatric patients

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