Enhancement of Neonatal Neutrophil Motility (Chemotaxis) with Adult Fresh Frozen Plasma

Eisenfeld, Leonard; Krause, Peter J.; Herson, Victor; Block, Charlotte; Schick, James B. M.; Maderazo, Eufronio G.

doi:10.1055/s-2007-994659

Cited by 11 publications

(6 citation statements)

References 2 publications

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“…One of the most remarkable indirect indications of the existence of an important factor in the serum of septic patients is that massive fresh plasma transfusions restore neutrophil function to newborns suffering from sepsis (9). The reduction of neutrophil migration has been described in experimental polymicrobial (3) and Gram-positive (4) sepsis, and the extent of the reduction was related to the intensity of sepsis.…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that serum factors may also be responsible for the inhibition of neutrophil migration, because massive fresh plasma transfusions restore neutrophil function in newborns suffering from sepsis (9). Serum factors have also been reported to play a role in the reduced neutrophil migration in other diseases, such as diabetes (10), cirrhosis (11), AIDS (12), and cancer (13).…”

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confidence: 99%

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Acute-phase protein α-1-acid glycoprotein mediates neutrophil migration failure in sepsis by a nitric oxide-dependent mechanism

Mestriner¹,

Spiller²,

Laure

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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The reduction of circulating neutrophil migration to infection sites is associated with a poor outcome of severe sepsis. ␣-1-Acid glycoprotein (AGP) was isolated from the sera of severely septic patients by HPLC and acrylamide gel electrophoresis and identified by mass spectrometry. Both the isolated protein and commercial AGP inhibited carrageenin-induced neutrophil migration into the rat peritoneal cavity when administered i.v. at a dose of 4.0 g per rat (95 pmol per rat). Analysis by intravital microscopy demonstrated that both proteins inhibited the rolling and adhesion of leukocytes in the mesenteric microcirculation. The inhibitory activity was blocked by 50 mg/kg aminoguanidine, s.c., and was not demonstrable in inducible nitric oxide synthase (iNOS) knockout mice. Incubation of AGP with neutrophils from healthy subjects induced the production of NO and inhibited the neutrophil chemotaxis by an iNOS/NO/cyclic guanosine 3,5-monophosphatedependent pathway. In addition, AGP induced the L-selectin shedding by neutrophils. The administration of AGP to rats with mild cecal ligation puncture sepsis inhibited neutrophil migration and reduced 7-day survival from Ϸ80% to 20%. These data demonstrate that AGP, an acute-phase protein, inhibits neutrophil migration by an NO-dependent process and suggest that AGP also participates in human sepsis.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Acute-phase protein α-1-acid glycoprotein mediates neutrophil migration failure in sepsis by a nitric oxide-dependent mechanism

Mestriner¹,

Spiller²,

Laure

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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“…Despite the evidence that cytokines mediate the failure of neutrophil migration, clinical trials using neutralizing antibodies against cytokines or soluble receptors did not show significant beneficial effects on septic patients (13). However, other serum factors may also be responsible for the inhibition of neutrophil migration because massive fresh plasma transfusions restore neutrophil function in newborns suffering from sepsis (14).…”

mentioning

confidence: 99%

Inhibition of Neutrophil Migration by Hemopexin Leads to Increased Mortality Due to Sepsis in Mice

Spiller

Costa²,

Souto³

et al. 2011

Am J Respir Crit Care Med

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“…First, a number of PMN abnormalities are known on the host defence system in high-risk premature neonates, such as relatively low activity of PMN adherence to vascular endothelia, aggregation and chemotaxis [5,6]. Eisenfeld et al reported that adult fresh frozen plasma enhanced impaired chemotaxis of neonatal PMN owing to supply of complement and fibronectin [33]. IVIG therapy which aims to compensate for the deficit of immunoglobulin could not be expected to restore such impairment of PMN functions to yield favourable clinical outcome and survival in such neonates [17,18].…”

Section: Discussionmentioning

confidence: 99%

Effect of immunoglobulin therapy on phagocytosis by polymorphonuclear leucocytes in whole blood of neonates

Fujiwara

Taniuchi

Hattori

et al. 1997

Clinical and Experimental Immunology

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SUMMARYThere has been some disagreement as to the clinical effect of intravenous immunoglobulin (IVIG) therapy on neonatal bacterial infections. We therefore evaluated the effect of IVIG therapy on neonatal polymorphonuclear leucocyte (PMN) functions by monitoring phagocytosis and hydrogen peroxide (H 2 O 2 ) production. Subjects were 10 mature neonates who had normal plasma levels (i.e. equal to adult plasma levels) of IgG and nine premature neonates who had lower plasma levels of IgG. Phagocytosis by PMN was measured using flow cytometric analysis of whole blood. Addition of g-globulin to the whole blood of mature neonates increased phagocytosis, but not significantly. Higher doses of added g-globulin (> 2·0 mg/ml, the concentration was expressed as that in the final reaction volume) decreased phagocytosis to under baseline level. In premature neonates addition of g-globulin increased phagocytosis and the significant maximum effect was observed with 0·5 mg/ml of the additional g-globulin.Higher doses of additional g-globulin (2·5 mg/ml) decreased phagocytosis to baseline level. Phagocytosis in four mature and four premature neonates was compared before and after 1 g/kg of IVIG therapy. Phagocytosis in mature neonates after IVIG therapy did not change compared with the pretreatment level. On the other hand, phagocytosis in premature neonates after IVIG therapy significantly increased compared with its pretreatment level. In both mature and premature neonates H 2 O 2 production following phagocytosis varied in parallel with changes of phagocytosis. The patterns of H 2 O 2 production following phagocytosis were essentially similar to those observed with phagocytosis. The above results are expected to form the basis for a rational indication for IVIG therapy against bacterial infections in neonates with low plasma IgG levels.

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Enhancement of Neonatal Neutrophil Motility (Chemotaxis) with Adult Fresh Frozen Plasma

Cited by 11 publications

References 2 publications

Acute-phase protein α-1-acid glycoprotein mediates neutrophil migration failure in sepsis by a nitric oxide-dependent mechanism

Acute-phase protein α-1-acid glycoprotein mediates neutrophil migration failure in sepsis by a nitric oxide-dependent mechanism

Inhibition of Neutrophil Migration by Hemopexin Leads to Increased Mortality Due to Sepsis in Mice

Effect of immunoglobulin therapy on phagocytosis by polymorphonuclear leucocytes in whole blood of neonates

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