Enhancement of Metallosphaera sedula Bioleaching by Targeted Recombination and Adaptive Laboratory Evolution

McCarthy, Samuel; Ai, Chenbing; Blum, Paul H.

doi:10.1016/bs.aambs.2018.03.002

Cited by 14 publications

(2 citation statements)

References 75 publications

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“…This last option points toward the possibility of an optimized bioleaching organism that manages the redox potential through the relative rates of sulfur and iron oxidation. Efforts to generate an optimized strain of M. sedula through laboratory evolution had moderate success in this regard (McCarthy, Ai and Blum 2018). While natural evolution of the ability to breakdown chalcopyrite may provide novel insights, tailoring an organism to mitigate surface passivation may require a more controlled approach.…”

Section: Biomining Applications Of Thermoacidophilesmentioning

confidence: 99%

The biology of thermoacidophilic archaea from the order Sulfolobales

Lewis

Recalde

Bräsen

et al. 2021

FEMS Microbiology Reviews

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Thermoacidophilic archaea belonging to the order Sulfolobales thrive in extreme biotopes, such as sulfuric hot springs and ore deposits. These microorganisms have been model systems for understanding life in extreme environments, as well as for probing the evolution of both molecular genetic processes and central metabolic pathways. Thermoacidophiles, such as the Sulfolobales, use typical microbial responses to persist in hot acid (e.g. motility, stress response, biofilm formation), albeit with some unusual twists. They also exhibit unique physiological features, including iron and sulfur chemolithoautotrophy, that differentiate them from much of the microbial world. Although first discovered more than 50 years ago, it was not until recently that genome sequence data and facile genetic tools have been developed for species in the Sulfolobales. These advances have not only opened up ways to further the probe novel features of these microbes, but have also paved the way for potential biotechnological applications. Discussed here are the nuances of the thermoacidophilic lifestyle of the Sulfolobales, including their evolutionary placement, cell biology, survival strategies, genetic tools, metabolic processes, and physiological attributes together with how these characteristics make thermoacidophiles ideal platforms for specialized industrial processes.

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Section: Biomining Applications Of Thermoacidophilesmentioning

confidence: 99%

The biology of thermoacidophilic archaea from the order Sulfolobales

Lewis

Recalde

Bräsen

et al. 2021

FEMS Microbiology Reviews

View full text Add to dashboard Cite

show abstract

“…In addition to the studies on adaptation and increase in resistance mentioned above, an interesting work on enhancement of M. sedula bioleaching should be mentioned [ 101 ]. In this work, laboratory adaptation and selection of the archaea M. sedula were accompanied with (1) genetic mutation analyses and (2) transcriptomics to study stress-induced gene expression.…”

Section: Genetic Modifications Of Leaching Microorganismsmentioning

confidence: 99%

Current Trends in Metal Biomining with a Focus on Genomics Aspects and Attention to Arsenopyrite Leaching—A Review

Абашина

Vainshtein

2023

Microorganisms

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The presented review is based on scientific microbiological articles and patents in the field of biomining valuable metals. The main attention is paid to publications of the last two decades, which illustrate some shifts in objects of interest and modern trends both in general and applied microbiology. The review demonstrates that microbial bioleaching continues to develop actively, despite various problems in its industrial application. The previous classic trends in the microbial bioleaching persist and remain unchanged, including (i) the search for and selection of new effective species and strains and (ii) technical optimization of the bioleaching process. Moreover, new trends were formed during the last decades with an emphasis on the phylogeny of leaching microbiota and on genomes of the leaching microorganisms. This area of genomics provides new, interesting information and forms a basis for the subsequent construction of new leaching strains. For example, this review mentions some changed strains with increased resistance to toxic compounds. Additionally, the review considers some problems of bioleaching valuable metals from toxic arsenopyrite.

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Development of a defined medium for the heterotrophic cultivation of Metallosphaera sedula

Sedlmayr,

Luger,

Pittenauer

et al. 2024

Extremophiles

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The heterotrophic cultivation of extremophilic archaea still heavily relies on complex media. However, complex media are associated with unknown composition, high batch-to-batch variability, potential inhibiting and interfering components, as well as regulatory challenges, hampering advancements of extremophilic archaea in genetic engineering and bioprocessing. For Metallosphaera sedula, a widely studied organism for biomining and bioremediation and a potential production host for archaeal ether lipids, efforts to find defined cultivation conditions have still been unsuccessful. This study describes the development of a novel chemically defined growth medium for M. sedula. Initial experiments with commonly used complex casein-derived media sources deciphered Casamino Acids as the most suitable foundation for further development. The imitation of the amino acid composition of Casamino Acids in basal Brock medium delivered the first chemically defined medium. We could further simplify the medium to 5 amino acids based on the respective specific substrate uptake rates. This first defined cultivation medium for M. sedula allows advanced genetic engineering and more controlled bioprocess development approaches for this highly interesting archaeon.

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Enhancement of Metallosphaera sedula Bioleaching by Targeted Recombination and Adaptive Laboratory Evolution

Cited by 14 publications

References 75 publications

The biology of thermoacidophilic archaea from the order Sulfolobales

The biology of thermoacidophilic archaea from the order Sulfolobales

Current Trends in Metal Biomining with a Focus on Genomics Aspects and Attention to Arsenopyrite Leaching—A Review

Development of a defined medium for the heterotrophic cultivation of Metallosphaera sedula

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