Enhancement of lipases enantioselectivity by entrapment in hydrophobic sol-gel materials: Influence of silane precursors and immobilization parameters

Péter, Fráncisc; Zarcula, Cristina; Kiss, Claudia; Csunderlik, Carol; Poppe, László

doi:10.1016/j.jbiotec.2007.07.187

Cited by 6 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The maximum conversion yield of 95.75% was obtained by NS88001 with hexane and methanol at the 16 h. [18,23,34] observed similar trends from crude tallow, waste cooking oil and vegetable oil. Freedman et al [32], Ma et al [35], Leung and Guo [36], Peter et al [37] and Eevera et al [38] reported that the rate of conversion of fatty acid esters increased with increases in reaction time as the reaction proceeds rapidly due to the initial mixing and dispersion of alcohol into the oil substrate and the activation of enzyme. Chen et al [23] reported that after alcohol was dispersed, it rapidly interacted with fatty acids giving a maximum conversion yield.…”

Section: Effect Of Reaction Timementioning

confidence: 99%

Effectiveness of Enzymatic Transesterification of Beeftallow Using Experimental Enzyme Ns88001 with Methanol and Hexane

Brooks¹

2013

Enz Eng

View full text Add to dashboard Cite

The high demand for fossil fuels and their limited supply have prompted the search for alternative renewable fuels such as biodiesel, bioethanol and biogas from biomass materials [1,2]. Biodiesel is a renewable fuel that can be used in compression-ignition engines instead of petroleum diesel [3,4]. It has several qualities over diesel including: being sulfur free, non-toxic, biodegradable and non-carcinogenic [5]. These characteristics make it more greener and eco-friendly than diesel [6-10]. There are many raw materials that can be used as a feedstock for the production of biodiesel including: plant oils, animal fats, microbial mass and waste materials [7]. The most popular plants used as a feedstock are jatropha, canola, coconut, cottonseed, groundnut, karanj, olive, palm, peanut, rapeseed, safflower, soybean and sunflower [3,7,10]. The most popular animal sources used as a feedstock are beef tallow, chicken fat, lamb fat, lard, yellow grease and hemp oil [3,11-13]. The waste materials include: waste cooking oil, greasy by-product from omega-3 fatty acid production and fish waste [7,10]. The main component of fats and oils are triacylglycerols (triglycerides) which are made of different types of fatty acids with one glycerol (glycerine) being the backbone. The types of fatty acids present in the triglycerides determine the fatty acids profile. Fatty acid profiles from plants and animal sources are different and each fatty acid has its own chemical and physical properties which can be a major factor influencing the properties of biodiesel [7,14]. Transesterification is a classic chemical process used to convert the vegetable oils and animal fats to biodiesel. Usually, a short chain alcohol is used with the feedstock to convert methyl esters and glycerin. The objective is to reduce the viscosity of oil by turning it into biodiesel [15]. Transesterification can proceed with one of three catalysts: acid, alkali and enzyme. With an acid catalyst, the proton is donated to the carbonyl group which makes it more reactive. A base catalyst is used to remove the proton from alcohol which makes the reactants more reactive. Both acid and alkali methods require more energy and a downstream processing step is required for removing the by-product (glycerin) [16]. An enzymatic catalyst cleaves the backbone of the glycerol which makes the reactants more reactive, giving the product without the need for a downstream processing step. The glycerol can be extracted easily and the energy required for the process is minimal. Objectives The main aim of present study was to optimize the enzymatic transesterification process for the production of biodiesel from

show abstract

Section: Effect Of Reaction Timementioning

confidence: 99%

Effectiveness of Enzymatic Transesterification of Beeftallow Using Experimental Enzyme Ns88001 with Methanol and Hexane

Brooks¹

2013

Enz Eng

View full text Add to dashboard Cite

show abstract

“…The most common gels used are: methylenebisacylamide, calcium alginate and kappacarrageenan (Klibanov, 1983). The procedure used to entrap the lipase is relatively simple, quite robust and easy to recover during continuous operation but the cost factor is not as low as adsorption (Meter et al, 2007). The biggest disadvantage to entrapment is the mass transfer limitation (Malcata et al, 1990).…”

Section: Immobilization Of Lipasementioning

confidence: 99%

Production of Biodiesel by Enzymatic Transesterification: Review

Ghaly¹,

Dave²,

Brooks³

et al. 2010

American Journal of Biochemistry and Biotechnology

224

108

View full text Add to dashboard Cite

Problem Statement:The research on the production of biodiesel has increased significantly in recent years because of the need for an alternative fuel which endows with biodegradability, low toxicity and renewability. Plant oils, animal fats, microalgal oils and waste products such as animal rendering, fish processing waste and cooking oils have been employed as feedstocks for biodiesel production. In order to design an economically and environmentally sustainable biodiesel production process, a proper understanding of the factors affecting the process and their relative importance is necessary. Approach: A comprehensive review of the literature on the subject of biodiesel production was carried out. Traditionally biodiesel has been produced using either acid or base catalysts. The multi-step purification of end products, wastewater treatment and energy demand of the conventional process has lead to search for alternative option for production of biodiesel. The use the enzyme lipase as a biocatalyst for the transesterification reaction step in biodiesel production has been extensively investigated. Lipase is produced by all living organisms and can be used intracellularly or extracellularly. Conclusion: To date, the most popular microbes used for their lipases have been filamentous fungi and recombinant bacteria. A summary of lipases used in transesterification and their optimum operating conditions is provided. In addition to the choice of lipase employed, factors which make the transesterification process feasible and ready for commercialization are: enzyme modification, the selection of feedstock and alcohol, use of common solvents, pretreatment of the lipase, alcohol to oil molar ratio, water activity/content and reaction temperature. Optimization of these parameters is necessary in order toreduce the cost of biodiesel production. Use of no/low cost waste materials as feedstocks will have double environmental benefits by reducing the environmental pollution potential of the wastes and producing an environmentally friendly fuel.

show abstract

Interfaces in Multiphase Polymer Systems

Marosi

2011

Handbook of Multiphase Polymer Systems

View full text Add to dashboard Cite

Enhancement of lipases enantioselectivity by entrapment in hydrophobic sol-gel materials: Influence of silane precursors and immobilization parameters

Cited by 6 publications

References 2 publications

Effectiveness of Enzymatic Transesterification of Beeftallow Using Experimental Enzyme Ns88001 with Methanol and Hexane

Effectiveness of Enzymatic Transesterification of Beeftallow Using Experimental Enzyme Ns88001 with Methanol and Hexane

Production of Biodiesel by Enzymatic Transesterification: Review

Interfaces in Multiphase Polymer Systems

Contact Info

Product

Resources

About