Enhancement of leaf rust resistance of Siberian winter wheat varieties by marker-assisted selection

Abstract

Cultivation of winter wheat varieties in the West Siberian region of Russia has competitive advantages compared to spring varieties: utilization of spring-summer moisture, early maturation and harvest and a high yield potential. The poor resistance of winter varieties to foliar diseases results in significant yield losses and facilitates the spread of pathogens to the spring wheat cultivars. The present study was conducted to evaluate the effectiveness of molecular markers specific for VRN-1 and Lr loci in selecting winter wheat genotypes resistant to leaf rust. The winter wheat cultivars Biyskaya ozymaya and Filatovka were crossed with spring wheat introgression lines 21-4 and 5366-180 and the spring wheat cultivar Tulaikovskaya 10 carrying LrTt2, LrAsp5 and Lr6Ai#2 loci from Triticum timopheevii, Aegilops speltoides and Thynopyrum intermedium, respectively. To identify winter wheat plants homozygous for target loci, F 2 populations were screened with functional markers to VRN-1 genes and with markers specific for alien genetic material. Based on the genotyping analysis of 371 F 2 plants a total of 44 homozygous genotypes with winter habit was identified. There were eight genotypes containing Lr loci among them. Evaluation of F 2-derived F 3-4 families for both seedling and adult resistance showed that only one F 3-4 family had moderate susceptible reaction type to the field population of leaf rust. Others ranged from nearly immune to resistant with severity of 5%. The data also indicated the utility of the VRN-1 allele-specific markers for detection of genotypes with winter habit without vernalization at early stages of plant breeding.

Marker-assisted selection and yield component assessment of spring wheat plants derived from crosses between spring and winter wheat cultivars

“…To identify plants carrying the LrAsp5 gene from Ae. speltoides, the Pr1 and Pr5 primers were used, their structure and PCR conditions for them as described in a work by Leonova et al (2017). Agriculturally important traits were assessed in F 3 plants on an experimental Abstract: The effectiveness of the use of marker-assisted selection for identification of spring growth-habit genotypes derived from crosses between soft winter wheat cultivars and the spring wheat introgression line 21-4 carrying the Ae.…”

Development and study of new spring wheat lines containing alien genetic material from Th. intermedium and Ae. speltoides

“…The degree of susceptibility was assessed using a modified Cobb's scale (Peterson et al, 1948). Screening of genotypes for the presence of the Lr6Ai = 2 and LrSp2 genes was performed using the Xicg6Ai = 2 and Xksum73 markers as previously described (Leonova et al, 2017;Adonina et al, 2018). Separation of PCR products was performed in 2 % agarose gel or on an ABI3130XL automatic sequencer in a 6 % denaturing polyacrylamide gel.…”

INLUENCE OF ALIEN GENETIC MATERIAL ON THE MANIFESTATION OF AGRONOMICALLY IMPORTANT TRAITS OF COMMON WHEAT (T. aestivum L.)

“…Молекулярные маркеры, разработанные для генов устойчивости и фрагментов транслокации, содержащих QTLs, эффективно применяются в схемах маркерориен тированной селекции, маркерконтролируемого отбора для интрогрессии и пирамидирования генов, получения генотипов с различной длиной фрагментов и ускоренно го создания доноров целевых локусов (Chen et al, 2005;Vida et al, 2009;Timonova et al, 2013;Tyagi et al, 2014;Leonova et al, 2017b).…”