Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases

Wang, Yayue; Xin, Yanjuan; Cao, Xupeng; Xue, Song

doi:10.1007/s11274-015-1817-2

Cited by 6 publications

(3 citation statements)

References 15 publications

(14 reference statements)

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“…397 The genus Pseudomonas presented two predominantly active OTUs in all analyzed samples, 411 belonging to Pseudomonas lingynensis (6-57% RA, similarity of 99.6%) and Pseudomonas 412 stutzeri (1-10% RA, similarity of 99-100%) ( Table A4). P. stutzeri constituted 9-10% RA ( Table 413 As aerobic or facultative-anaerobic bacteria were present in the microcosm, oxygen availability 432 as a co-substrate could be explained by: (i) the occurrence of nitrite-driven processes that would 433 supplement molecular oxygen to monooxygenase activity (Ettwig et al, 2010) as well as to the 434 cometabolism for the degradation of halomethanes; ii) the availability of O2 from chlorite 435 dismutase activity in P. stutzeri (Cladera et al, 2006;Schaffner et al, 2015); iii) in the presence 436 of L-2-haloacid dehalogenases, known to obtain an oxygen atom of the solvent water, in detected 437 species including A. aquaticus (Kumar et al, 2016), P. stutzeri (Wang et al, 2015), and Rhizobium 438 sp. RC1 (Adamu et al, 2016) (the last genus with 1-3% RA in all analyzed samples).…”

Section: Active Microbial Populations Assessment 376mentioning

confidence: 99%

Vitamin B12 effects on chlorinated methanes-degrading microcosms: Dual isotope and metabolically active microbial populations assessment

Rodríguez-Fernández

Torrentó

Guivernau

et al. 2018

Science of The Total Environment

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Field-derived anoxic microcosms were used to characterize chloroform (CF) and carbon tetrachloride (CT) natural attenuation to compare it with biostimulation scenarios in which vitamin B was added (B/pollutant ratio of 0.01 and 0.1) by means of by-products, carbon and chlorine compound-specific stable-isotope analysis, and the active microbial community through 16S rRNA MiSeq high-throughput sequencing. Autoclaved slurry controls discarded abiotic degradation processes. B catalyzed CF and CT biodegradation without the accumulation of dichloromethane, carbon disulphide, or CF. The carbon isotopic fractionation value of CF (ƐC) with B was -14±4‰, and the value for chlorine (ƐCl) was -2.4±0.4‰. The carbon isotopic fractionation values of CT (ƐC) were -16±6 with B, and -13±2‰ without B; and the chlorine isotopic fractionation values of CT (ƐCl) were -6±3 and -4±2‰, respectively. Acidovorax, Ancylobacter, and Pseudomonas were the most metabolically active genera, whereas Dehalobacter and Desulfitobacterium were below 0.1% of relative abundance. The dual C-Cl element isotope slope (Λ=ΔδC/ΔδCl) for CF biodegradation (only detected with B, 7±1) was similar to that reported for CF reduction by Fe(0) (8±2). Several reductive pathways might be competing in the tested CT scenarios, as evidenced by the lack of CF accumulation when B was added, which might be linked to a major activity of Pseudomonas stutzeri; by different chlorine apparent kinetic isotope effect values and Λ which was statistically different with and without B (5±1 vs 6.1±0.5), respectively. Thus, positive B effects such as CT and CF degradation catalyst were quantified for the first time in isotopic terms, and confirmed with the major activity of species potentially capable of their degradation. Moreover, the indirect benefits of B on the degradation of chlorinated ethenes were proved, creating a basis for remediation strategies in multi-contaminant polluted sites.

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Section: Active Microbial Populations Assessment 376mentioning

confidence: 99%

Vitamin B12 effects on chlorinated methanes-degrading microcosms: Dual isotope and metabolically active microbial populations assessment

Rodríguez-Fernández

Torrentó

Guivernau

et al. 2018

Science of The Total Environment

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“…P. aeruginosa HS9 could degrade 69% HBCDs in the MSM in 14 days, but removed 88% HBCDs in the plant soil (Huang et al 2019). It indicated that the different factor in growing environment of bacteria could affect their transformation ability, in corresponding to biodehalogenation of other organic halogenated compounds (Wang et al 2015). Diastereoisomers-speci c biotransformation of HBCD was also observed in other functional strains.…”

Section: Discussionmentioning

confidence: 92%

Biotransformations of HBCDs by Rhodococcus Strain Stu-38 and Identification of Transformation Products

Yu¹,

Luo²,

Li³

et al. 2021

Preprint

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1, 2, 5, 6, 9, 10-Hexabromocyclododecanes (HBCDs) are new brominated flame retardants causing serious environmental pollution. Dozens of degradative bacteria have been found with capacity to transform HBCDs. In the present study, an aerobic functional bacterium Rhodococcus strain stu-38 was isolated from enriched culture of mangrove sediment using HBCDs as carbon source. This strain could stereoselectively transform HBCDs, the removal rate was α-＞γ-＞β-HBCD in the mineral salt medium, but was β-＞α- and γ-HBCD in the growth medium, and it selectively transformed γ- HBCD in the seawater. Transformation rate of strain stu-38 was lower than other functional strains, however, seven potential debrominated products of HBCDs were identified by using GC-MS. These debrominated products, included dibromocyclododecadiene, bromocyclododecadienol and bromocyclododecatriene were formed through reductive debromination, hydrolytic debromination and dehydrobromination. Overall, Rhodococcus sp. stu-38 diastereoisomer-specifically transformed HBCDs to various debrominated products in the different cultural media, which highlighted the complicated stereoselective biotransformation of HBCDs.

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“…[3], in addition to causing serious environmental pollution owing to their toxicity, their toxic breakdown products, persistence and bioaccumulation in the food chain and water bodies [4]. These compounds are poisonous and very difficult to degrade [5].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterisation of the Degradation Potential of 2,2-Dichloropropionate (2,2-Dcp) by Bacillus Amyloliquefaciens From Gebeng

ALİYU

Adamu

Wahab

et al. 2022

International Journal of Life Sciences and Biotechnology

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Most halogenated compounds such as α-halocarboxylic acid (αHCA) are potential carcinogens that are toxic and widely liberated in both aquatic and terrestrial ecosystem. 2,2dichloropropionate (2,2-DCP) is a synthetic halogenated compound commonly used in agricultural activities as an herbicide. This study is focused on the isolation and characterization of dehalogenase producing bacteria capable of utilizing 2,2dichloropropionate as the sole carbon and energy source from soil sample obtained from the industrial hub of Gebeng (Pahang), Malaysia. An enrichment culture technique supplemented with 2,2-DCP was used to isolate the bacteria. A pure culture strain designated as strain FDS grew well in media containing 20 mM 2,2-DCP at a temperature of 30˚C exhibiting a cell doubling time of 22.21hours and a maximum rate of chloride ion release of 0.345mmol/L suggesting this is the optimum concentration for growth. Based on microscopic observations, biochemical characteristics and phylogenetic analysis of the 16S rRNA gene sequence, FDS was identified as Bacillus amyloliquefaciens with 98% homology. A literature survey revealed no report regarding the role of B. amyloliquefaciens on pesticide biodegradation as well as on 2,2-DCP, therefore the current isolate was designated Bacillus amyloliquefaciens FDS.

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Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases

Cited by 6 publications

References 15 publications

Vitamin B12 effects on chlorinated methanes-degrading microcosms: Dual isotope and metabolically active microbial populations assessment

Vitamin B12 effects on chlorinated methanes-degrading microcosms: Dual isotope and metabolically active microbial populations assessment

Biotransformations of HBCDs by Rhodococcus Strain Stu-38 and Identification of Transformation Products

Isolation and Characterisation of the Degradation Potential of 2,2-Dichloropropionate (2,2-Dcp) by Bacillus Amyloliquefaciens From Gebeng

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