Enhancement of immune responses to an HBV DNA vaccine by electroporation

Potentiation of an anthrax DNA vaccine with electroporation

“…For instance, recent reports have shown that EP can induce reactivity against a broader range of epitopes than conventional vaccine administration. Thus far, this has been demonstrated only with T cell epitopes [7][8][9]. If this is also the case for B cell epitopes, this could include epitopes that are critical for neutralizing activity.…”

“…Vaccine delivery was performed by an i.m. administration of plasmid DNA using Ichor's TriGrid™ EP technology as previously reported [7]. The intraelectrode spacing of the TriGrid electrode array used in mice, rats, and rabbits was 2.5 mm, 3.0mm, and 6.0mm respectively.…”

“…Therefore, it is thought that methods for enhancing intracellular delivery of DNA vaccines could improve immunogenicity and enable the technology for clinical use [4]. In that regard, electroporation (EP) appears promising, because it is a potent in vivo method for intracellular gene delivery [5] as well as a very effective means for DNA vaccination [6][7][8][9].…”

Refinement of a DNA based Alzheimer disease epitope vaccine in rabbits

“…Vaccine delivery was performed by intramuscular administration of 0.5 ml (1 mg/ml) plasmid DNA using Ichor's TDS-IM technology as previously reported. 47 Rabbits were immunized four times biweekly and blood was collected 12-14 d after each immunization.…”

Potential of Transfected Muscle Cells to Contribute to DNA Vaccine Immunogenicity

“…One microgram of total RNA was reverse-transcribed in first strand buffer (50 mM Tris-HCl (pH 7.5), 75 mM KCl, and 2.5 mM MgCl 2 ), containing 25 g/ml oligo(dT) [12][13][14][15][16][17][18] , 200 U of Malony leukemia virus reverse transcriptase, 2 mM dNTP, and 10 mM DTT. The reaction was conducted at 42°C for 1 h. A standard PCR was performed on 1 l of the cDNA synthesis using the primer pairs in Table 1.…”