Enhancement of IL-18 expression by Paecilomyces tenuipes

Abstract

Paecilomyces tenuipes is a popular medicinal mushroom, and has received extensive attention for medical application because of its various physiological activities. However, there is limited information about the anticancer and immunomodulatory activities of Paecilomyces tenuipes . This study attempted to evaluate the effect of an extract from the cultured fruiting bodies of Paecilomyces tenuipes (PTE) on the expression of the interleukin-18 (IL-18) gene in rat pheochromocytoma (PC) 12 cells and rat brain. Related mRNA levels were determined by reverse transcription-polymerase chain reaction (RT-PCR). Protein levels were measured by Western blot and immunohistochemistry. Our results demonstrated that PTE induced IL-18 gene expression both in vitro and in vivo.Treatment of PC12 cells and rat brain cells with 10 lg/ml and 20 mg/kg PTE, respectively, yielded significant increases of IL-18 levels. Significantly, IL-18-immunoreactive neurons were detected in the Purkinje cells of the cerebellum. IL18-immunohistochemical staining was markedly enhanced in animals treated with PTE compared to findings in the untreated controls. These results suggest that PTE could be a potential candidate as an immune activator or anticancer drug.

De�novo transcriptome sequencing of Paecilomyces tenuipes revealed genes involved in adenosine biosynthesis

“…Due to its various biological and pharmacological activities, P. tenuipes can be used as a substitute for Cordyceps sinensis in health supplements. This fungus contains numerous biologically active constituents, in which adenosine is of importance (3)(4)(5)(6). Adenosine displays various pharmacological activities, including cardioprotective, anti-inflammatory and anticancer properties (7)(8)(9)(10).…”

Antidepressant-like activities of extracts of the fungus Paecilomyces tenuipes M98

“…Entomogenous fungi are considered to be an ecologically highly specialized group of microorganisms, due to their plentiful pharmaceutically active compounds [6]. Paecilomyces tenuipes, one of the most popular entomogenous fungi in China, has various bioactive substances and bioactivities, including hypoglycemic, immunomodulatory and anti-tumor [7]. In the previous study, we had successfully obtained a mutant of P. tenuipes, named P. tenuipes M98, and proved that the mutant had high content of active substances and excellent anti-diabetic activity and safety.…”

Application of Near Infrared Spectroscopy in Quality Control of Paecilomyces Tenuipes

“…Paecilomyces tenuipes has traditionally been used as health food and folk medicine in Japan, Korea and China because of the various biological and pharmacological activities, such as immunomodulatory, anti-tumor, anti-angiogenic, antidepressant and hypoglycemic [3][4][5]. Some valuable components, such as polysaccharide, nucleoside, potein, cordyceps acid, cordycepin, tenuipesine, sterol, trichothecanes and cyclopeptide have been found from Paecilomyces tenuipes [6][7][8][9][10].…”